Nanoplastic production procedure for scientific purposes: PP, PVC, PE-LD, PE-HD, and PS

International audienceStudies on the environmental impact of nanoplastics face challenges in plastic analysis and a scarcity of nanoplastic materials necessary for the development of analytical techniques and experiments on biota impact. Here we provide detailed procedures for obtaining nanoparticles suspended in water for the most commonly used polymers: Polypropylene (PP), Polyvinylchloride (PVC), Low-and High-Density Polyethylene (PE-LD, PE-HD), and Polystyrene (PS). We dissolved larger size material to reprecipitate nanoparticles. For all plastic types, we obtained nanoparticles with a size between 50 and 300 nm, and a mainly spherical morphology. We verified that no irreversible agglomeration or coalescence of the particles occurred after 5 days of storage. The concentrations obtained in the final carrier solution were of the order of 10 9 particles mL-1. To prevent the persistence of reagents in the final carrier solution, a filtration step was implemented at the end of the process. The method proved unsuitable for Polyethylene Terephthalate (PET)

An Experimental Comparison Between FTIR and Raman Microspectroscopy Applied to the Morphological Analysis of Microplastics in Drinking Water

Fourier-Transform InfraRed micro-spectroscopy (µFTIR) was applied to the microplastic (MP) analysis of drinking water samples taken from the inlet and outlet of a groundwater-fed waterworks located in Denmark. The outcome of the µFTIR analysis was then compared to that of Raman micro-spectroscopy (µRaman) applied to the same set of samples. Overall, µFTIR was more effective to analyse the &amp;gt;50 µm MPs, whilst µRaman the 1–50 µm MP fraction.</p

Do drinking water plants retain microplastics? An exploratory study using Raman micro-spectroscopy

The retainment of microplastics (MPs) down to 1 μm by a Danish drinking water plant fed with groundwater was quantified using Raman micro-spectroscopy (μRaman). The inlet and outlet were sampled in parallel triplicates over five consecutive days of normal activity. For each triplicate, approximately 1 m3 of drinking water was filtered with a custom-made device employing 1 μm steel filters. The MP abundance was expressed as MP counts per liter (N/L) and MP mass per liter (pg/L), the latter being estimated from the morphological parameters provided by the μRaman analysis. Hence the treated water held on average 1.4 MP counts/L, corresponding to 4 pg/L. The raw water entering the sand filters held a higher MP abundance, and the overall efficiency of the treatment was 43.2% in terms of MP counts and 75.1% in terms of MP mass. The reason for the difference between count-based and mass-based efficiencies was that 1–5 μm MP were retained to a significantly lower degree than larger ones. Above 10 μm, 79.6% of all MPs were retained by the filters, while the efficiency was only 41.1% below 5 μm. The MP retainment was highly variable between measurements, showing an overall decreasing tendency over the investigated period. Therefore, the plastic elements of the plant (valves, sealing components, etc.) likely released small-sized MPs due to the mechanical stress experienced during the treatment. The sub-micron fraction (0.45–1 μm) of the samples was also qualitatively explored, showing that nanoplastics (NPs) were present and that at least part hereof could be detected by μRaman

Treating wastewater for microplastics to a level on par with nearby marine waters

Retention of microplastics (MPs) at the third largest wastewater treatment plant (WWTP) in Sweden was investigated. The plant is one of the most modern and advanced of its kind, with rapid sand filter for tertiary treatment in combination with mechanical, biological, and chemical treatment. It achieved a significantly high treatment efficiency, which brought the MP concentration in its discharge on par with concentrations measured in marine waters of the same region. This novel data shows that properly designed modern WWTPs can reduce the MP content of sewage down to background levels measured in the receiving aquatic environment. Opposite to current understanding of the retention of MP by WWTPs, a modern and well-designed WWTP does not have to be a significant point source for MP. MPs were quantified at all major treatment steps, including digester inlet and outlet sludge. MPs sized 10–500 µm were analyzed by a focal plane array based micro-Fourier transform infrared (FPA-µFTIR) microscopy, a hyperspectral imaging technique, while MPs above 500 µm were analyzed by Attenuated Total Reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy. Mass was estimated from the hyperspectral images for MPs &lt;500 µm and from microscope images &gt;500 µm. The overall treatment efficiency was in terms of MP counts 99.98 %, with a daily input of 6.42 × 1010 and output of 1.04 × 107 particles. The mass removal efficiency was 99.99 %. The mechanical part of the treatment, the pre-treatment, and primary stages, reduced both the MP counts and mass by approximately 71 %. The combined biological treatment, secondary settling, and final polishing with rapid sand filtration removed nearly all the remaining 29 %. MPs became successively smaller as they passed the different treatment steps. The digester inlet received 1.04 × 1011 MPs daily, while it discharged 9.96 × 1010 MPs, causing a small but not significant decrease in MP counts, with a corresponding MP mass reduction of 9.56 %