Genetisches und zytogenetisches Monitoring chemisch-exponierter Beschaeftigtengruppen in der mikroelektronischen und chemischen Industrie. T. 2: Literatur Schlussbericht

SIGLEAvailable from TIB Hannover: F96B1526+a / FIZ - Fachinformationszzentrum Karlsruhe / TIB - Technische InformationsbibliothekBundesministerium fuer Bildung, Wissenschaft, Forschung und Technologie, Bonn (Germany)DEGerman

Stoffumwandlung mit Biokatalysatoren. Modifizierung der Glucoamylase von Arxula adeninivorans in Hefe Bericht aus Forschung und Entwicklung. Endbericht

By immunoscreening the glucoamylase gene (GAA-gene) was isolated from the yeast Arxula adeninivorans. The gene could be identified on a DNA-fragment on chromosome 2 from Arxula and comprises 1872 bp. Within the N-terminal end of the protein the first 16 amino acids are the signal sequence for entering the endomembrane system. Comparing the amino acid sequence from the glucoamylase with those of other fungal glucoamylases it is shown that our glucoamylase has a high homology with the glucoamylase from Rhizopus oryzae (32.6%). No homology could be detected to the glucoamylase from Schwanniomyces occidentalis. By using Saccharomyces cerevisiae promoters in autonomously replicating plasmids it was possible to express the isolated GAA-gene in S. cerevisiae and Kluyveromyces lactis. The transformants secreted more than 90% of the expressed glucoamylase into the culture medium. The glucoamylases secreted by all three microorganism has similar biochemical properties but is expressed and secreted more rapidly in the S. cerevisiae transformants than in A. adeninivorans or K. lactis transformants. (orig.)SIGLEAvailable from TIB Hannover: F95B1130+a / FIZ - Fachinformationszzentrum Karlsruhe / TIB - Technische InformationsbibliothekBundesministerium fuer Bildung, Wissenschaft, Forschung und Technologie, Bonn (Germany)DEGerman

Genetisches und zytogenetisches Monitoring chemisch-exponierter Beschaeftigtengruppen in der mikroelektronischen und chemischen Industrie. T. 1. Textband Schlussbericht

The peripheral blood lymphocytes isolated from workers of different exposure situations - (1) plasma etching industry, (2) final chlorination of polyvinylchloride and (3) synthesis of monochlorodimethylether - and control subjects were examined for chromosomal mutations, sister chromatid exchanges, gene mutations and DNA-adducts. After careful consideration of the exposure environment in a microelectronic plant and the recognition of the critical exposure situation it was possible to introduce several improvements that led to measurable decrease in the number of chromosomal aberrations observed in lymphocytes. In the group of workers performing final chlorination of polyvinylchloride it could be shown that the DNA-adducts were the most sensitive parameter. In contrast, in the case of workers engaged in the synthesis of ethers chromosomal damage was determinated to be the most sensitive one. The results of the study have revealed the possibilities of improving the safety of the working place. Besides obtaining valuable information about the genotoxic and cancer risk of chemical exposure at the workingplace, information about the general health of the workers was obtained. (orig.)SIGLEAvailable from TIB Hannover: F96B1525+a / FIZ - Fachinformationszzentrum Karlsruhe / TIB - Technische InformationsbibliothekBundesministerium fuer Bildung, Wissenschaft, Forschung und Technologie, Bonn (Germany)DEGerman

Versuche zur Erzeugung von Duplikationen sowie zur physikalischen Kartierung von RFLP-Sonden im Gerstenchromosom 5 (1H) mit Hilfe von Translokationen Schlussbericht

Short DNA sequences not yet detectable by in situ hybridization can be localized physically by PCR with chromosme-specific DNA used as a template. PCR with DNA from 10 microisolated prophase nuclei or 20 chromosomes results, after amplification with sequence-specific primers, in detectable fragments indicative of the single-copy RFLP probes. This technique allowed for the first time to construct a cytogenetically based physical RFLP map for a barley chromosome. In barley about 400 translocation breakpoints were cytologically localized on Giemsa-banded chromosomes. This provides a sample of different breakpoints sufficient to construct the basic structures of cytogenetically-based RFLP maps for all barley chromosomes. The presented technique is especially useful to find out those genetic markers which flank the proximal chromosome regions characterized by suppressed recombination. (WEN)Available from TIB Hannover: F96B419+a / FIZ - Fachinformationszzentrum Karlsruhe / TIB - Technische InformationsbibliothekSIGLEBundesministerium fuer Bildung, Wissenschaft, Forschung und Technologie, Bonn (Germany)DEGerman

Stoffumwandlung mit Biokatalysatoren. Modifizierung der Glucoamylase von Arxula adeninivorans in Hefe. Anhang Bericht aus Forschung und Entwicklung. Endbericht

SIGLEAvailable from TIB Hannover: F95B1131+a / FIZ - Fachinformationszzentrum Karlsruhe / TIB - Technische InformationsbibliothekBundesministerium fuer Bildung, Wissenschaft, Forschung und Technologie, Bonn (Germany)DEGerman

Molekularbiologische Untersuchungen zur Erhoehung der photosynthetischen Stoffproduktion bei Pflanzen. Untersuchungen zur Beeinflussung photorespiratorischer Prozesse in Pflanzen Schlussbericht

SIGLEAvailable from TIB Hannover: DtF QN1(34,49) / FIZ - Fachinformationszzentrum Karlsruhe / TIB - Technische InformationsbibliothekBundesministerium fuer Bildung, Wissenschaft, Forschung und Technologie, Bonn (Germany)DEGerman

Neues Wirts-Vektor-System fuer Produktsynthesen in der Hefe Arxula adeninivorans Ls3 Schlussbericht

Available from TIB Hannover: D.Dt.F.QN1(19,2) / FIZ - Fachinformationszzentrum Karlsruhe / TIB - Technische InformationsbibliothekSIGLEBundesministerium fuer Forschung und Technologie (BMFT), Bonn (Germany)DEGerman

Semiautomatische Kartierung von landwirtschaftlich wichtigen Merkmalen bei Nutzpflanzen Schlussbericht

Available from TIB Hannover: DtF QN1(28,38) / FIZ - Fachinformationszzentrum Karlsruhe / TIB - Technische InformationsbibliothekSIGLEBundesministerium fuer Forschung und Technologie (BMFT), Bonn (Germany)DEGerman

Etablierung eines in vitro-Modells neuronaler Zellen. Teilprojekt 1: Zell-und molekularbiologische Charakterisierung definierter neuronaler Zellpopulationen aus embryonalen Stamm (ES)-Zellen Abschlussbericht

SIGLEAvailable from TIB Hannover: F01B1569 / FIZ - Fachinformationszzentrum Karlsruhe / TIB - Technische InformationsbibliothekBundesministerium fuer Bildung, Wissenschaft, Forschung und Technologie, Bonn (Germany)DEGerman

Physische Kartierung des Gerstengenoms - Integration genetischer und physischer Chromosomenkarten Schlussbericht

SIGLEAvailable from TIB Hannover: F98B2+a / FIZ - Fachinformationszzentrum Karlsruhe / TIB - Technische InformationsbibliothekBundesministerium fuer Bildung, Wissenschaft, Forschung und Technologie, Bonn (Germany)DEGerman