2 research outputs found
Green Formulation Strategy for Preparing Oil-in-Water Emulsions via Lipase-Catalyzed Transesterification
Formulation
of submicronic diglyceride-in-water emulsions was carried
out without addition of synthetic surfactant in case of commercial
caprylic/capric diglyceride. Sugar surfactant was prepared by contacting
the oil with a concentrated aqueous solution of sorbitol (70 wt %)
containing lipase AY. Interfacial lipase-catalyzed transesterification
took place and led to limited but sufficient amounts of sorbitol ester
and monoglyceride, which accumulated in the oil. The enzyme-treated
oil could be easily separated from the aqueous phase and used for
preparing oil-in-water emulsion without adding any other surfactant.
A stable emulsion was obtained for at least 7 days and exhibited better
stability at 60 °C than Tween 20-stabilized emulsion
Lipase-catalyzed synthesis of sucrose monoester: Increased productivity by combining enzyme pretreatment and non-aqueous biphasic medium
Sucrose monocaprate was synthesized by carrying out a lipase-catalyzed transesterification in a non-aqueous biphasic medium. Vinyl caprate was mechanically dispersed into a solution of sucrose in DMSO. The use of DMSO allowed increasing sucrose concentration up to 0.7. M (in DMSO). The denaturing effect of DMSO on lipase was avoided by pretreatment of lipase by pH adjustment in the presence of crown ether. This pretreatment maintained a significant catalytic activity which led to 0.2. M sucrose monoester within 1. h at 50. °C, which represented higher productivity than already reported. Detailed structural characterization revealed that only monoester was recovered and the 2-O-acylated sucrose monocaprate was the major isomer in the final product