The effect of INA treatment on ³H-<i>L</i>-arginine uptake by <i>M</i>. <i>gallisepticum</i>.

<p><i>M</i>. <i>gallisepticum</i> Rlow cells (0.2 mg cell protein/mL) were treated with INA followed by UV irradiation and tested for ³H-<i>L</i>-arginine uptake. Open symbols, INA treated cells; closed symbols, control of untreated cells.</p

Transmission electron micrographs of INA treated <i>M</i>. <i>gallisepticum</i>.

<p>Washed <i>M</i>. <i>gallisepticum</i> Rlow preparations were treated with 200 μM INA, irradiated by UV light and analyzed by transmission electron microscopy as described in Materials and methods. (A), untreated <i>M</i>. <i>gallisepticum</i>; (B), <i>M</i>. <i>gallisepticum</i> treated with INA at 37°C; (C), <i>M</i>. <i>gallisepticum</i> treated with INA at 4°C.</p

The effect of treatment with iodoazidonaphtalenes followed by UV photoinduction on the viability of <i>M</i>. <i>gallisepticum</i>.

<p><i>M</i>. <i>gallisepticum</i> Rlow log-phase (exponential) culture were harvested, washed twice and resuspended in TN buffer to a final concentration of 0.2 mg/mL cell protein. The cells were treated with 200μM 1, 5-iodonaphthylazide (INA), 1-azidonaphtalene (AzNAP), 1,5-diazidonaphtalene (DAN), 1,5-diiodonaphtalene (DINAP) and 1-azido 3,5-diiodonaphtalene (DINA) at room temperature, irradiated by UV for 2 min and viability was determined as described in Materials and Methods.</p

Additional file 2: Table S1. of The mysterious orphans of Mycoplasmataceae

Genomic GC content and genic GC3 content for annotated species of Mycoplasma, Spiroplasma, and Ureaplasma. (DOCX 22 kb

Additional file 3: of The mysterious orphans of Mycoplasmataceae

Description of selected bacterial genomes. Difference between COGs and ORFans. (XLSX 314 kb

The effect of INA treatment of <i>M</i>. <i>gallisepticum</i> cells on ATPase and NADH₂ dehydrogenase activities.

<p><i>M</i>. <i>gallisepticum</i> strain R low cells grown in Hayflick’s medium were harvested, washed and treated with INA followed by UV irradiation. Cells treated with 4% paraformaldehyde (PFA) served as a negative control. The treated cells were disrupted by ultrasonic treatment [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0120462#pone.0120462.ref012" target="_blank">12</a>] and ATPase and NADH₂ dehydrogenase activities were determined in the cell extract as described in Materials and Methods. The results are presented as specific activities and are means ± standard deviation of three independent experiments.</p><p>*Specific activity expressed as μmoles of inorganic phosphorous released per mg cell protein per 30 min.</p><p>**Specific activity expressed as the decrease in absorbency at 340 nm per mg cell protein per min.</p><p>The effect of INA treatment of <i>M</i>. <i>gallisepticum</i> cells on ATPase and NADH₂ dehydrogenase activities.</p

Confocal micrographs depicting the interaction of INA treated <i>M</i>. <i>gallisepticum</i> with cRBC.

<p>cRBS were infected at a MOI of 100 with untreated <i>M</i>. <i>gallisepticum</i> cells (A) or with <i>M</i>. <i>gallisepticum</i> cells treated with INA followed by UV irradiation (B).</p

The effect of INA treatment on the membrane protein and on the lipoprotein profiles of <i>M</i>. <i>gallisepticum</i> Rlow.

<p>Western immunoblot analysis of isolated <i>M</i>. <i>gallisepticum</i> membranes (A) and the lipoprotein fraction (B) obtained from untreated (I) or INA treated cells (II).</p

The effect of UV irradiation on the viability of <i>M</i>. <i>gallisepticum</i>.

<p>The effect of UV irradiation on the viability of <i>M</i>. <i>gallisepticum</i>.</p