1 research outputs found
Pruning wound protection products induce alterations in the wood mycobiome profile of Grapevines
Fungal pathogens involved in grapevine trunk diseases (GTDs) may infect grapevines
throughout their lifetime, from nursery to vineyard, via open wounds in stems, canes or roots. In
vineyards, pruning wound protection products (PWPPs) offer the best means to reduce the chance
of infection by GTD fungi. However, PWPPs may affect non-target microorganisms that comprise
the natural endophytic mycobiome residing in treated canes, disrupting microbial homeostasis and
indirectly influencing grapevine health. Using DNA metabarcoding, we characterized the endophytic
mycobiome of one-year-old canes of cultivars Cabernet Sauvignon and Syrah in two vineyards in
Portugal and Italy and assessed the impact of established and novel PWPPs on the fungal communities
of treated canes. Our results reveal a large fungal diversity (176 taxa), and we report multiple genera
never detected before in grapevine wood (e.g., Symmetrospora and Akenomyces). We found differences
in mycobiome beta diversity when comparing vineyards (p = 0.01) but not cultivars (p > 0.05). When
examining PWPP-treated canes, we detected cultivar- and vineyard-dependent alterations in both
alpha and beta diversity. In addition, numerous fungal taxa were over- or under-represented when
compared to control canes. Among them, Epicoccum sp., a beneficial genus with biological control
potential, was negatively affected by selected PWPPs. This study demonstrates that PWPPs induce
alterations in the fungal communities of grapevines, requiring an urgent evaluation of their direct
and indirect effects on plants health with consideration of factors such as climatic conditions and
yearly variations, in order to better advise viticulturists and policy makers.info:eu-repo/semantics/publishedVersio