Statistical properties of dust far-infrared emission

The description of the statistical properties of dust emission gives important constraints on the physics of the interstellar medium but it is also a useful way to estimate the contamination of diffuse interstellar emission in the cases where it is considered a nuisance. The main goals of this analysis of the power spectrum and non-Gaussian properties of 100 micron dust emission are 1) to estimate the power spectrum of interstellar matter density in three dimensions, 2) to review and extend previous estimates of the cirrus noise due to dust emission and 3) to produce simulated dust emission maps that reproduce the observed statistical properties. The main results are the following. 1) The cirrus noise level as a function of brightness has been previously overestimated. It is found to be proportional to instead of ^1.5, where is the local average brightness at 100 micron. This scaling is in accordance with the fact that the brightness fluctuation level observed at a given angular scale on the sky is the sum of fluctuations of increasing amplitude with distance on the line of sight. 2) The spectral index of dust emission at scales between 5 arcmin and 12.5 degrees is =-2.9 on average but shows significant variations over the sky. Bright regions have systematically steeper power spectra than diffuse regions. 3) The skewness and kurtosis of brightness fluctuations is high, indicative of strong non-Gaussianity. 4) Based on our characterization of the 100 micron power spectrum we provide a prescription of the cirrus confusion noise as a function of wavelength and scale. 5) Finally we present a method based on a modification of Gaussian random fields to produce simulations of dust maps which reproduce the power spectrum and non-Gaussian properties of interstellar dust emission.Comment: 13 pages, 13 figures. Accepted for publication in A&

A quantitative model of the initiation of DNA replication in Saccharomyces cerevisiae predicts the effects of system perturbations.

BackgroundEukaryotic cell proliferation involves DNA replication, a tightly regulated process mediated by a multitude of protein factors. In budding yeast, the initiation of replication is facilitated by the heterohexameric origin recognition complex (ORC). ORC binds to specific origins of replication and then serves as a scaffold for the recruitment of other factors such as Cdt1, Cdc6, the Mcm2-7 complex, Cdc45 and the Dbf4-Cdc7 kinase complex. While many of the mechanisms controlling these associations are well documented, mathematical models are needed to explore the network's dynamic behaviour. We have developed an ordinary differential equation-based model of the protein-protein interaction network describing replication initiation.ResultsThe model was validated against quantified levels of protein factors over a range of cell cycle timepoints. Using chromatin extracts from synchronized Saccharomyces cerevisiae cell cultures, we were able to monitor the in vivo fluctuations of several of the aforementioned proteins, with additional data obtained from the literature. The model behaviour conforms to perturbation trials previously reported in the literature, and accurately predicts the results of our own knockdown experiments. Furthermore, we successfully incorporated our replication initiation model into an established model of the entire yeast cell cycle, thus providing a comprehensive description of these processes.ConclusionsThis study establishes a robust model of the processes driving DNA replication initiation. The model was validated against observed cell concentrations of the driving factors, and characterizes the interactions between factors implicated in eukaryotic DNA replication. Finally, this model can serve as a guide in efforts to generate a comprehensive model of the mammalian cell cycle in order to explore cancer-related phenotypes

Distorted images : attitudes towards the Micmac in Nova Scotia, 1788-1900

iii, 223 leaves ; 28 cm.Includes abstract.Includes bibliographical references (leaves 212-223).This paper, through an examination of Nova Scotian literature, analyses changing attitudes towards the Micmac in Nova Scotia from 1788 to 1900. The roots of stereotypes held by non-natives are explored as well as the influence of stereotypes on interaction between the two groups and on public policy. Nova Scotian literature of the period reveals two contradictory images of the native people, one based on the concept of the Noble Savage, the other rooted in tales of bestial wild men who lived outside the bounds of civil society. These images, the Noble Savage and the Demonic Savage, shaped the attitudes of Nova Scotians towards the Micmac. From 1788 to 1850, the intellectual framework which justified a discriminatory social hierarchy was based on theories of historical, social and moral development. From 1851 to 1900, rapid social, economic and political change left Nova Scotians, feeling more uncertain about their place in the world. As their hierarchical society crumbled, a new justification for discrimination was provided by emerging sciences. Scientific speculation contributed to the development of another image, the Vanishing Indian. The wide spread belief that the Indians would soon disappear strengthened assimilative efforts. (Abstract shortened by UMI.

Improving our understanding of the Spitzer Space Telescope's pointing drifts

Spitzer observations of exoplanets routinely yield photometric accuracies of better than one part in 10,000. However, the attainable precision is limited in part by pointing drifts, which have the effect of moving the target to less stable or less-well characterized regions of Spitzer’s IRAC detector arrays. Here we examine a large sample of observing sequences in an effort to identify the causes of these pointing drifts. We find that short term and higher order drifts are correlated on various time scales to the temperatures of components in and around the spacecraft bus, and are most likely due to very slight angular displacements of the star trackers. Despite the constraints imposed by a limited pool of targets, such pointing drifts are best mitigated by optimal scheduling, minimizing large and/or lengthy excursions in telescope pitch angle within 24 hours of a high-precision photometry sequence. Such an effort is currently being initiated by the Spitzer Science Center

Commercial-off-the-shelf simulation package interoperability: Issues and futures

Commercial-Off-The-Shelf Simulation Packages (CSPs) are widely used in industry to simulate discrete-event models. Interoperability of CSPs requires the use of distributed simulation techniques. Literature presents us with many examples of achieving CSP interoperability using bespoke solutions. However, for the wider adoption of CSP-based distributed simulation it is essential that, first and foremost, a standard for CSP interoperability be created, and secondly, these standards are adhered to by the CSP vendors. This advanced tutorial is on an emerging standard relating to CSP interoperability. It gives an overview of this standard and presents case studies that implement some of the proposed standards. Furthermore, interoperability is discussed in relation to large and complex models developed using CSPs that require large amount of computing resources. It is hoped that this tutorial will inform the simulation community of the issues associated with CSP interoperability, the importance of these standards and its future

Association of the Dictyostelium 30 kDa actin bundling protein with contact regions

\u27Contact regions\u27 are plasma membrane domains derived from areas of intercellular contact between aggregating Dictyostelium amebae (H.M. Ingalls et al. (1986). Proc. Nat. Acad. Sci. USA 83, 4779). Purified contact regions contain a prominent actin-binding protein with an M(r) of 34,000. Immunoblotting with monoclonal antibodies identifies this polypeptide as a 34,000 M(r) actin-bundling protein (known as 30 kDa protein), previously shown to be enriched in filopodia (M. Fechheimer (1987). J. Cell Biol. 104, 1539). About four times more 30 kDa protein by mass is associated with contact regions than is found in total plasma membranes isolated from aggregating cells. In agreement with these observations, immunostaining of the 30 kDa protein in aggregating cells reveals a prominent localization along the plasma membrane at sites of intercellular contact. By contrast, alpha-actinin does not appear to be significantly enriched at sites of cell to cell contact. Binding experiments using purified plasma membranes, actin and 30 kDa protein indicate that the 30 kDa protein is associated with the plasma membrane primarily through interactions with actin filaments. Calcium ions are known to decrease the interaction of actin with 30 kDa protein in solution. Surprisingly, membrane-associated complexes of actin and the 30 kDa protein are much less sensitive to dissociation by micromolar levels of free calcium ions than are complexes in solutions lacking membranes

Seven temperate terrestrial planets around the nearby ultracool dwarf star TRAPPIST-1

One aim of modern astronomy is to detect temperate, Earth-like exoplanets that are well suited for atmospheric characterization. Recently, three Earth-sized planets were detected that transit (that is, pass in front of) a star with a mass just eight per cent that of the Sun, located 12 parsecs away. The transiting configuration of these planets, combined with the Jupiter-like size of their host star—named TRAPPIST-1—makes possible in-depth studies of their atmospheric properties with present-day and future astronomical facilities. Here we report the results of a photometric monitoring campaign of that star from the ground and space. Our observations reveal that at least seven planets with sizes and masses similar to those of Earth revolve around TRAPPIST-1. The six inner planets form a near-resonant chain, such that their orbital periods (1.51, 2.42, 4.04, 6.06, 9.1 and 12.35 days) are near-ratios of small integers. This architecture suggests that the planets formed farther from the star and migrated inwards. Moreover, the seven planets have equilibrium temperatures low enough to make possible the presence of liquid water on their surfaces