Spearman rank order correlations between vascular variables and clinical and biochemical data in the whole group of PHPT patients and controls.

<p>Statistically significant correlations; * = p<0.05, ** = p<0.01, *** = p<0.001.</p><p>AIx = augmentation index, PWV_ao = aortic pulse wave velocity, IMT_rad = intima media thickness in right radial artery, IMT_cca = intima media thickness in both left and right common carotid artery, IM-GSM = intima media grayscale median in both left and right common carotid artery, SBP = systolic blood pressure, bpm = beats per minutes, P = plasma, S = serum, S-Ca++ = ionized calcium, PTH = parathyroid hormone, S-25-OH-D = 25 hydroxyvitamin D, TG = triglycerides, Apo = apolipoprotein.</p

Clinical data for healthy controls and patients with mild primary hyperparathyroidism before and after parathyroidectomy.

a<p>n = 47. Values are shown as means±SD. F = female, HR = heart rate, bpm = beats per minute, SBP = systolic blood pressure, DBP = diastolic blood pressure, MAP = mean arterial pressure, BSA = body surface area, BMI = body mass index.</p

Stepwise regression analyses with indices of vascular function as dependent variables, and demographic data as independent variables in the whole group of PHPT patients and controls.

<p>AIx = augmentation index, PWVao = aortic pulse wave velocity, IMTcca = intima media thickness in both left and right common carotid artery, IMTrad = intima media thickness in right radial artery, IM-GSM = intima media grayscale median in both left and right common carotid artery, SBP = systolic blood pressure.</p

Augmentation index and ultrasound measurements for healthy controls and patients with mild primary hyperparathyroidism before and after parathyroidectomy.

a<p>n = 47,</p>b<p>n = 43–45,</p>c<p>n = 36–41. Values are shown as means±SD. F = female, AIx = augmentation index, PWV_ao = aortic pulse wave velocity, Ao SBP = aortic systolic blood pressure, Ao DBP = aortic diastolic blood pressure, IMT_rad = intima media thickness in right radial artery, LD_rad = lumen diameter in right radial artery, IMT_cca = intima media thickness in both left and right common carotid artery, LD_cca = lumen diameter in both left and right common carotid artery, IM-GSM = intima media grayscale median in both left and right common carotid artery.</p

Bland-Altman plots of inter-observer variability.

<p>Measurements of the common carotid artery intima media thickness (IMT_cca) are illustrated in the upper panel, and of the grayscale median (IM-GSM) in the lower panel.</p

Biochemical data for healthy controls and patients with mild primary hyperparathyroidism before and after parathyroidectomy.

a<p>n = 47,</p>b<p>n = 44–46.</p>c<p>One patient had CRP 35.40 mg/L at follow-up, after excluding the patient, mean hs-CRP was 1.26±1.09 mg/L, p<0.01. Values are shown as means±SD. F = female, P = plasma, S = serum, PTH = parathyroid hormone, S-Ca++ = ionised calcium, S-25-OH-D = 25 hydroxyvitamin D, GFR = glomerular filtration rate. P-hsCRP = high sensitivity C-reactive protein, TG = triglycerides, Apo = apolipoprotein, VWF = von Willebrand factor antigen, PAI-1 = plasminogen activator inhibitor-1 activity, IGF-1 = insulin like growth factor.</p

Stepwise regression analyses with indices of vascular function as dependent variables, and demographic and biochemical data as independent variables in the whole group of PHPT patients and controls.

<p>AIx = augmentation index, PWV_ao = aortic pulse wave velocity, IMT_cca = intima media thickness in both left and right common carotid artery, IMT_rad = intima media thickness in right radial artery, IM-GSM = intima media grayscale median in both left and right common carotid artery, SBP = systolic blood pressure, PTH = parathyroid hormone, P = plasma, S = serum, S-Ca++ = ionized calcium, PTH = parathyroid hormone, S-25-OH-D = 25 hydroxyvitamin D.</p

Localisation of PRLr expression to lysosomes in normal parathyroid rim and to enlarged lysosomes in parathyroid tumour tissue.

<p>A) Immunohistochemistry with PRLrI showing “ring-like” cytoplasmic structures and cytoplasmic reactivity. B) Immunohistochemistry of PRLrI showing cytoplasmic granuale and cytoplasmic reactivty. C and D) Analysis of “ring like” structures and cytoplasmic granulae by flourescent immunohistochemistry. Images show one parathyroid tumour (C) and normal parathyroid rim (D), stained with DAPI (blue), anti-PRLrI (red, upper right) and anti-SCARB2 (green lysosomal marker, lower left) separately and in overlay (lower right and upper left).</p

Immunohistochemical analysis of PRLr expression using the PRLrI antibody

<p>. The photomicrographs show parathyroid tumour tissues (A–C) and negative control (D). Parathyroid tumours are shown with immunostaining of cytoplasmic granulae and cytoplasm (A), of cytoplasm only (B), and of cell membrane and cytoplasm (C).</p

Western blot analysis of protein expression for GSK3β and isoforms of the prolactin receptor.

<p>A) Detection of 80 kDa and 60/70 kDa products with the PRLrI antibody in normal tissues, T47D cells and parathyroid tumours, and 60/70 kDa N-glycosylated PRLr products with the gPRLr antibody in parathyroid tumours. B) Expression of total GSK3β (left) as well as Ser9-phosphorylated GSK3β (right) in parathyroid tumours and normal tissue.</p