20 research outputs found
ТЕХНОГЕННІ РОДОВИЩА ТА ЇХ КЛАСИФІКАЦІЯ
На багатьох гірничодобувних підприємствах тих, що забезпечують мінера-льною сировиною чорну і кольорову металургію України та країн СНД, виник-ла проблема з розвіданими запасами. Резерв запасів багатьох видів корисних копалини на експлуатованих родовищах недостатній для забезпечення повної проектної потужності. Стан сировинних баз багатьох найважливіших гірничо-добувних регіонів і підприємств, що діють, різко погіршав у зв'язку з висна-женням запасів, зниженням їх якісних і економічних характеристик ускладнен-ням умов відробітку в результаті тривалої і інтенсивної експлуатації раніше освоєних родовищ. Основною причиною ситуації, що створилася, можна назва-ти зниження фінансування геологорозвідувальних робіт для освоєння нових ро-довищ необхідної мінеральної сировини
An isolated perfused pig heart model for the development, validation and translation of novel cardiovascular magnetic resonance techniques
Abstract Background Novel cardiovascular magnetic resonance (CMR) techniques and imaging biomarkers are often validated in small animal models or empirically in patients. Direct translation of small animal CMR protocols to humans is rarely possible, while validation in humans is often difficult, slow and occasionally not possible due to ethical considerations. The aim of this study is to overcome these limitations by introducing an MR-compatible, free beating, blood-perfused, isolated pig heart model for the development of novel CMR methodology. Methods 6 hearts were perfused outside of the MR environment to establish preparation stability. Coronary perfusion pressure (CPP), coronary blood flow (CBF), left ventricular pressure (LVP), arterial blood gas and electrolyte composition were monitored over 4 hours. Further hearts were perfused within 3T (n = 3) and 1.5T (n = 3) clinical MR scanners, and characterised using functional (CINE), perfusion and late gadolinium enhancement (LGE) imaging. Perfusion imaging was performed globally and selectively for the right (RCA) and left coronary artery (LCA). In one heart the RCA perfusion territory was determined and compared to infarct size after coronary occlusion. Results All physiological parameters measured remained stable and within normal ranges. The model proved amenable to CMR at both field strengths using typical clinical acquisitions. There was good agreement between the RCA perfusion territory measured by selective first pass perfusion and LGE after coronary occlusion (37% versus 36% of the LV respectively). Conclusions This flexible model allows imaging of cardiac function in a controllable, beating, human-sized heart using clinical MR systems. It should aid further development, validation and clinical translation of novel CMR methodologies, and imaging sequences.</p
DNA methylation biomarkers predict progression-free and overall survival of metastatic renal cell cancer (mRCC) treated with antiangiogenic therapies.
VEGF-targeted therapy increases both the progression-free (PFS) and overall survival (OS) of patients with metastasized renal cell cancer (mRCC). Identification of molecular phenotypes of RCC could improve risk-stratification and the prediction of the clinical disease course. We investigated whether gene-specific DNA hypermethylation can predict PFS and OS among patients undergoing anti-VEGF-based therapy. Primary tumor tissues from 18 patients receiving targeted therapy were examined retrospectively using quantitative methylation-specific PCR analysis of CST6, LAD1, hsa-miR-124-3, and hsa-miR-9-1 CpG islands. PFS and OS were analyzed for first-line and sequential antiangiogenic therapies using the log rank statistics. Sensitivity and specificity were determined for predicting first-line therapy failure. Hypermethylation of CST6 and LAD1 was associated with both a shortened PFS (log rank p = 0.009 and p = 0.004) and OS (p = 0.011 and p = 0.043). The median PFS observed for the high and low methylation groups of CST6 and LAD1 was 2.0 vs.11.4 months. LAD1 methylation had a specificity of 1.0 (95% CI 0.65-1.0) and a sensitivity of 0.73 (95% CI 0.43-0.90) for the prediction of first-line therapy. CST6 and LAD1 methylation are candidate epigenetic biomarkers showing unprecedented association with PFS and OS as well as specificity for the prediction of the response to therapy. DNA methylation markers should be considered for the prospective evaluation of larger patient cohorts in future studies
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Pathogenicity, Fungicide Resistance, and Genetic Variability of Phytophthora rubi Isolates from Raspberry (Rubus idaeus) in the Western United States
Root rot of raspberry (Rubus idaeus), thought to be primarily caused
by Phytophthora rubi, is an economically important disease in the
western United States. The objectives of this study were to determine
which Phytophthora species are involved in root rot, examine the efficacy
of different isolation methods (cane, root, and root/soil baiting
with young raspberry plants), and determine if pathogenicity, fungicide
resistance, and/or genetic variation exists among P. rubi isolates collected
from raspberry fields in Washington, Oregon, and California. Of
275 samples, direct isolation from cane material resulted in a greater
number of P. rubi isolates (39%), whereas root/soil baiting yielded the
least (11%). Sequencing of the internal transcribed spacer region of
210 of the total 597 collected Phytophthora isolates showed that all but
one isolate (identified as P. bisheria) were P. rubi. Results of the pathogenicity
and fungicide resistance to mefenoxam comparing 14 total
isolates from Washington, Oregon, and California showed that isolates
were similarly virulent against red raspberry and the EC₅₀ frequency
distributions showed no significant difference. These results, combined
with amplified fragment length polymorphism results show that P. rubi
isolates from Washington, Oregon, and California represent one large
mixed population. This work provides novel insights into the isolation
and biology of P. rubi in western U.S. raspberry production systems