5 research outputs found
<i>PRNP</i> sequence of the reindeer used in this study compared with mule deer, white-tailed deer, Rocky Mountain elk, and moose.
<p>Reindeer are polymorphic at codons 2 (M/V), 129 (S/G), 138 (S/N), and 169 (M/V). <i>PRNP</i> polymorphisms have also been reported in Rocky Mountain elk (132 M/L), mule deer (20 D/G, 225 S/F), white-tailed deer (95 Q/H, 96 G/S, 116 A/G, 226 Q/R, and 230 Q/L) and moose 209 (M/I) <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0039055#pone.0039055-Baeten1" target="_blank">[11]</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0039055#pone.0039055-ORourke1" target="_blank">[41]</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0039055#pone.0039055-Wilson1" target="_blank">[44]</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0039055#pone.0039055-Heaton1" target="_blank">[45]</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0039055#pone.0039055-Jewell1" target="_blank">[46]</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0039055#pone.0039055-ORourke2" target="_blank">[47]</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0039055#pone.0039055-Brayton1" target="_blank">[48]</a>.</p
Western immunoblot of PrP<sup>res</sup> in the brainstem of reindeer compared to elk and white-tailed deer CWD inocula.
<p>Reindeer 12 (lane 4) and reindeer 47 (lane 5) developed terminal CWD at 18.5 and 20 mpi. Lane 1– molecular weight marker (kDa), lane 2– CWD in elk, lane 3– CWD in white-tailed deer, lane 4– CWD in reindeer 12, lane 5– CWD in reindeer 47, lane 6– negative control elk.</p
Tissue distribution of PrP<sup>CWD</sup> and histopathology in clinically affected reindeer orally inoculated with CWD from white-tailed deer.
<p>IHC detection of PrP<sup>CWD</sup> using MAb F99/97.6.1 demonstrates: (A) immunolabeling associated with tingible body macrophages and follicular dendritic cells in a RAMALT biopsy taken at 13.4 mpi, (C) punctate staining in cardiomyocytes, (D) widespread particulate deposits in the dorsal vagal nucleus, (E) immunolabeling within a ganglion adjacent to the submandibular salivary gland, (F) granular PrP<sup>CWD</sup> in submucosal and myenteric plexuses of the ileum and (G) amongst glomerular lymphocytic infiltrates. (B) Haematoxylin and eosin stained section of the dorsal vagal nucleus reveals vacuolation of the neuropil. (H) PET blot of the obex demonstrating abundant widespread PrP<sup>CWD</sup> filling the dorsal vagal nucleus and surrounding nuclei. Bars, 150 µm (A); 65 µm (B,D,E); 24 µm (C,G); 92 µm (F); 2.4 mm (H).</p
PrP<sup>CWD</sup> distribution in tissues of reindeer orally inoculated with brain tissue from CWD-infected white-tailed deer.
a<p>PrP<sup>CWD</sup> was detected by IHC with MAb F99/97.6.1 and a commercially available TSE ELISA kit and results are expressed as PrP<sup>CWD</sup> present (+) or not detected (−).</p><p>RAMALT, recto-anal mucosa-associated lymphoid tissue; LN, lymph node.</p
Summary of results from reindeer orally inoculated with CWD from elk or white-tailed deer.<sup>a</sup>
a<p>ELISA  =  enzyme-linked immunosorbent assay; IHC  =  immunohistochemistry; LN  =  lymph node; mpi  =  months post-inoculation; NA  =  data not available; RAMALT  =  Recto-anal mucosa associated lymphoid tissue; WB  =  Western blot; WTD  =  White-tailed deer.</p>b<p>Time (mpi) to first clinical signs of neurological disease if observed.</p>c<p>Deduced amino acids at codons 2-129-138-169. V =  Valine; M =  Methionine; G =  Glycine; S =  Serine; N =  Asparagine.</p>d<p>IHC conducted on RAMALT or obex tissue using mAb F99. The time of last RAMALT sampling is listed in parentheses (mpi) for animals with a negative IHC result, while the time of the first positive RAMALT biopsy is listed for the positive animals.</p>e<p>Conducted on obex or retropharyngeal lymph node and reported as positive (+) or negative (–) with optical density (OD) in parentheses from a commercially available ELISA kit (negative OD cut-off  = 0.21).</p>f<p>Animal 17 remains alive at 26 mpi with no evidence of clinical signs or PrP<sup>CWD</sup> in RAMALT biopsy.</p