Levels of ω5-gliadins and HMW-GS in flour and isolated HMW-GS fractions of the wheat cvs. Akteur, Contra and Apache and the respective protein yields in the HMW-GS fractions.

1<p>Including the glutenin-bound (ωb-) gliadins.</p

Relative average molecular masses (M_r) and corresponding proteins detected in the isolated HMW-GS fractions of the cvs. Akteur, Contra and Apache as determined by RP-HPLC-ESI-MS with the mobile phase containing 0.1% (v/v) TFA.

1<p>Standard deviations are given between brackets.</p>2<p>Based on M_r agreement with proteins from the wheat storage proteome <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0058682#pone.0058682-Lagrain1" target="_blank">[5]</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0058682#pone.0058682-Dupont1" target="_blank">[36]</a>.</p

Comparison of the theoretical relative molecular mass (M_r) of HMW-GS calculated from their known amino acid sequences with their corresponding M_r determined by RP-HPLC-ESI-MS.

1<p>Based on results by Lagrain et al. <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0058682#pone.0058682-Lagrain1" target="_blank">[5]</a>.</p>2<p>No gene or protein sequence has been reported for Dx3.</p

SDS-PAGE of isolated HMW-GS fractions from three wheat cultivars (cvs.).

<p>The subunit composition of wheat cvs. Contra (lane 2), Akteur (lane 3) and Apache (lane 4) is indicated with numbers according to the current nomenclature system <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0058682#pone.0058682-Payne2" target="_blank">[4]</a>. Protein M_r markers are given in lane 1 (from top: myosin, β-galactosidase, bovine serum albumin, ovalbumin, carbonic anhydrase).</p

Mass spectra of wheat HMW-GS Ax1 and ω5-gliadin.

<p>The spectra are taken from the average of scans under the peak with retention time 6.2 min from the base peak mass chromatogram after RP-HPLC-ESI-MS with 0.1% (v/v) formic acid in the mobile phase. The simulated mass spectra obtained by maximum entropy deconvolution are shown as insets.</p

Base peak mass chromatogram from RP-HPLC-ESI-MS of the isolated HMW-GS fraction of wheat cv. Akteur.

<p>The mobile phase contains 0.1% (v/v) TFA. HMW-GS are labeled with numbers according to the current nomenclature system <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0058682#pone.0058682-Payne2" target="_blank">[4]</a>.</p

Relative average molecular masses (M_r) and corresponding proteins present in the isolated HMW-GS fractions of the cv. Akteur as determined by RP-HPLC-ESI-MS with the mobile phase containing 0.1% (v/v) formic acid.

1<p>Standard deviations are given between brackets.</p>2<p>Based on M_r agreement with proteins from the wheat storage proteome <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0058682#pone.0058682-Lagrain1" target="_blank">[5]</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0058682#pone.0058682-Mamone1" target="_blank">[23]</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0058682#pone.0058682-Muccilli1" target="_blank">[24]</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0058682#pone.0058682-Dupont1" target="_blank">[36]</a>.</p