Spectrum of lactobacillus species present in healthy vagina of Indian women

Background & objectives: Lactobacilli are depleted in vagina of women suffering from recurring episodes of bacterial vaginosis with vaginal pH≥5. With the objective of making available probiotic lactobacilli for replenishment in such women, a study was undertaken to isolate and characterize the Lactobacilli present in women with eco-healthy vagina in Delhi. No information is so far available on the species of Lactobacilli resident in vagina of women in India. Methods: Vaginal swabs were taken from 80 women with informed consent after ethical approval and grown in MRS broth. Gram-positive, catalase-negative bacilli generating about 200 bp amplicon by PCR with Lactobacillus genus specifi c primers were further characterized by employing species specifi c primers followed by sequencing of 16S rDNA. Isolates of the same species were differentiated by random amplifi ed polymorphic DNA (RAPD) profi les. Results: The predominant species isolated were L. reuteri present in 26 (32.5%) women, L. fermentum in 20 (25%), and L. salivarius in 13 (16.25%) women. Sequencing of 16S rDNA of 20 isolates showed that except for two isolates of L. plantarum, sequences of the remaining agreed well with PCR identification. None of the isolates had similar RAPD profile. Interpretation & conclusions: Our fi ndings showed lactobacilli species present in healthy vagina of women in India differ from those reported from other countries. This information would be useful to development of probiotic tablets seeking to replenish the missing lactobacilli for reproductive health of women in India

Histopathological examination of the placenta in normal and SGA pregnancy and its association with neonatal outcome

Objective: To study histopathological characteristics of the placenta in SGA pregnancies and to associate it with neonatal outcomes. Material and methods: A total of 200 placentae were examined.100 from the study group (SGA pregnancies) and 100 from the low-risk control group. After delivery, they were examined for any gross lesions and weighed. They were then sent for histopathological analysis. The results were then tabulated and compared between the two groups (study and control). Results: On studying the histopathology of the placenta we found abnormalities in 73 % of placentae in the study group as compared to 34% in the control group showing a significant association of placental pathology with FGR (p< 0.05).Salient histopathological findings in the study group were villous infarcts (52%), fetal thrombotic arteriopathy (11%), chronic villitis (7%), perivillous fibrin deposit (49%), decreased vascularity (45%), diffuse calcification (35%), syncytial knots (38%). We found that 82% of neonates were delivered before 37 completed weeks in the study group which was a significant finding (p<0.01). The finding of 72% of neonates in the study group with birth weight <2 kg was expected due to FGR babies. This finding was statistically significant. Our study failed to demonstrate any significant relation between major placental lesions and depression of APGAR score except for perivillous fibrin deposition which showed significant relation (p<0.05). On comparing the placental lesions with decreased fetal weight (<1.5kg) we found decreased vascularity (p<0.05) closely related to decreased fetal weight (p<0.05).The other lesions failed to show any significant relationship between the two (chi-square two-tailed test). Conclusion: We found that though SGA foetuses are more commonly associated with histological placental abnormalities, it cannot be established whether these abnormalities certainly contribute to FGR. Malperfusion lesions of the placenta show a close association with not only FGR but decreased fetal weight and poor neonatal outcomes. We also found the association of perivillous fibrin and decreased vascularity significantly related to adverse neonatal outcomes