DISEMINASI PEMBELAJARAN STRUKTUR DAN FUNGSI BIOMOLEKUL PROTEIN BAGI GURU WILAYAH MGMP-1 JAKARTA TIMUR

Materi biokimia di SMA dipelajari pada kelas XII dengan salah satu pembahasan mengenai biomolekul protein. Pada pandemi COVID-19, terjadi perubahan sistem pembelajaran yang membutuhkan berbagai inovasi agar pembelajaran berlangsung efektif. Program pengabdian pada masyakarat ini, melakukan pengembangan pembelajaran struktur dan fungsi biomolekul protein bagi guru MGMP Wilayah Jakarta Timur-1 melalui desiminiasi elektonik modul Struktur dan Fungsi Protein hasil penelitian sebelumnya serta praktikum berbasis bahan rumahan dengan teknik blended learning. Pelaksanaan P2M yang dilakukan bersama Tim UNJ pada Guru-guru Kimia MGMP Jakarta Timur wilayah 1 berjalan dengan lancar. Peserta sangat antusias mengikuti materi yang disampaikan dan ikut berkontribusi dalam pelaksanaan dan mengamati praktikum uji kualitatif asam amino dan protein menggunakan bahan-bahan yang ada disekitar rumah, ramah lingkungan, dan sekaligus menerapkan konsep green chemistry. Berdasarkan hasil umpan balik, 90% peserta merasa puas dengan penyampaian materi yang disampaikan, memperoleh wawasan baru, relevan dengan yang diharapkan, dapat diimplementasikan di sekolah, dan menimbulkan ide baru untuk mendesain pembelajaran yang inovatif. Sehingga diharapkan kegiatan pengabdian kepada masyarakat yang dilakukan oleh dosen Universitas Negeri Jakarta bekerjasama dengan MGMP wilayah Jakarta Timur 1 sebagai wilayah binaan yang meliputi kecamatan Cakung, Duren Sawit, Jatinegara, Matraman, dan Pulogadung, dapat bermanfaat dalam menyebarluaskan IPTEKS dan meningkatkan kompetensi guru

The Potential of grxB Gene for Detection of C. sakazakii in Infant Formula Milk Using Real-Time Polymerase Chain Reaction

Cronobacter sakazakii is one of the bacteria that causes food poisoning that contaminates infant formula. This pathogen causes necrotizing enterocolitis, sepsis, and meningitis in infants or neonates with reported case fatality rates ranging from 40% to 80%. Therefore, it is necessary to develop fast and accurate detection of C. sakazakii in infant formula milk. This research aims to develop a method for detecting C. sakazakii bacteria using real-time PCR with high sensitivity, specificity, and accuracy. A rapid detection method using real-time PCR with the target gene grxB successfully detects the presence of C. sakazakii DNA in artificially contaminated formula milk. The results of the real-time PCR test showed that C. sakazakii DNA with a concentration of 53 ng/µL could be amplified by the grxB gene primer pair with a Ct value of 12 and a Tm value of 85.8ºC. The specificity test showed that the grxB primer could differentiate between target and some non-target bacteria. The sensitivity test showed the ability of the grxB primer to detect the smallest concentration of 3,392 pg/µL with a Ct of 24,06. Based on the results obtained, it can be concluded that the grxB primer has the potential to be used as rapid detection method for C. sakazakii bacteria in infant formula using real-time PCR

Validation of the Detection Kit for Pathogenic Bacteria Salmonella typhi Causes Food Poisoning with Real Time Polymerase Chain Reaction

Salmonella typhi is a gram-negative bacteria that causes food poisoning. Salmonella typhi bacteria can cause typhus, which can produce lipopolysaccharide complex endotoxin, which plays an important role in the pathogenesis oftyphoid fever. Cases of food poisoning are still common and are one of the causes of death and death in Indonesia. In 2019, there were 77 cases of food poisoning in Indonesia. A fast, accurate, and specific detection method is needed to detect poisoning. In previous studies, the optimum conditions and formulas for detecting these bacteria have been obtained using Real Time PCR. The results of previous studies are used as the basis for the development of a prototypedetection kit. In this study, validation was carried out, which aimed to confirm the results of previous studies so that a reproducible and accurate product for the detection of Salmonella typhi bacteria could be obtained. The results of this study showed that fim-C primers for Salmonella typhi were amplified at 95 base pairs (bp) with an annealing temperature of 60ºC and a standard DNA concentration of 50 ng/µL. The results of the Real Time PCR confirmation test of Salmonellatyphi bacteria at a pre-denaturation of 3 minutes with a concentration of 10 pmol obtained the Ct value according to thestandard with previous studies. The Ct value obtained was 13.96 for S. typhi bacteria. Based on the results obtained, it can be conclude that the condition validation stage for pure cultures was successfully carried out by producing consistentand reproducible data

Validation of the Detection Kit for Pathogenic Bacteria Salmonella typhi Causes Food Poisoning with Real Time Polymerase Chain Reaction

Salmonella typhi is a gram-negative bacteria that causes food poisoning. Salmonella typhi bacteria can cause typhus, which can produce lipopolysaccharide complex endotoxin, which plays an important role in the pathogenesis of typhoid fever. Cases of food poisoning are still common and are one of the causes of death and death in Indonesia. In 2019, there were 77 cases of food poisoning in Indonesia. A fast, accurate, and specific detection method is needed to detect poisoning. In previous studies, the optimum conditions and formulas for detecting these bacteria have been obtained using Real Time PCR. The results of previous studies are used as the basis for the development of a prototype detection kit. In this study, validation was carried out, which aimed to confirm the results of previous studies so that a reproducible and accurate product for the detection of Salmonella typhi bacteria could be obtained. The results of this study showed that fim-C primers for Salmonella typhi were amplified at 95 base pairs (bp) with an annealing temperature of 60ºC and a standard DNA concentration of 50 ng/µL. The results of the Real Time PCR confirmation test of Salmonella typhi bacteria at a pre-denaturation of 3 minutes with a concentration of 10 pmol obtained the Ct value according to thestandard with previous studies. The Ct value obtained was 13.96 for S. typhi bacteria. Based on the results obtained, it can be conclude that the condition validation stage for pure cultures was successfully carried out by producing consistentand reproducible data

The Potential of

Cronobacter sakazakii is one of the bacteria that causes food poisoning that contaminates infant formula. This pathogen causes necrotizing enterocolitis, sepsis, and meningitis in infants or neonates with reported case fatality rates ranging from 40% to 80%. Therefore, it is necessary to develop fast and accurate detection of C. sakazakii in infant formula milk. This research aims to develop a method for detecting C. sakazakii bacteria using real-time PCR with high sensitivity, specificity, and accuracy. A rapid detection method using real-time PCR with the target gene grxB successfully detects the presence of C. sakazakii DNA in artificially contaminated formula milk. The results of the real-time PCR test showed that C. sakazakii DNA with a concentration of 53 ng/µL could be amplified by the grxB gene primer pair with a Ct value of 12 and a Tm value of 85.8ºC. The specificity test showed that the grxB primer could differentiate between target and some non-target bacteria. The sensitivity test showed the ability of the grxB primer to detect the smallest concentration of 3,392 pg/µL with a Ct of 24,06. Based on the results obtained, it can be concluded that the grxB primer has the potential to be used as rapid detection method for C. sakazakii bacteria in infant formula using real-time PCR

Validation of the Detection Kit for Pathogenic Bacteria

Salmonella typhi is a gram-negative bacteria that causes food poisoning. Salmonella typhi bacteria can cause typhus, which can produce lipopolysaccharide complex endotoxin, which plays an important role in the pathogenesis oftyphoid fever. Cases of food poisoning are still common and are one of the causes of death and death in Indonesia. In 2019, there were 77 cases of food poisoning in Indonesia. A fast, accurate, and specific detection method is needed to detect poisoning. In previous studies, the optimum conditions and formulas for detecting these bacteria have been obtained using Real Time PCR. The results of previous studies are used as the basis for the development of a prototypedetection kit. In this study, validation was carried out, which aimed to confirm the results of previous studies so that a reproducible and accurate product for the detection of Salmonella typhi bacteria could be obtained. The results of this study showed that fim-C primers for Salmonella typhi were amplified at 95 base pairs (bp) with an annealing temperature of 60ºC and a standard DNA concentration of 50 ng/µL. The results of the Real Time PCR confirmation test of Salmonellatyphi bacteria at a pre-denaturation of 3 minutes with a concentration of 10 pmol obtained the Ct value according to thestandard with previous studies. The Ct value obtained was 13.96 for S. typhi bacteria. Based on the results obtained, it can be conclude that the condition validation stage for pure cultures was successfully carried out by producing consistentand reproducible data

Validation of the Detection Kit for Pathogenic Bacteria

Salmonella typhi is a gram-negative bacteria that causes food poisoning. Salmonella typhi bacteria can cause typhus, which can produce lipopolysaccharide complex endotoxin, which plays an important role in the pathogenesis of typhoid fever. Cases of food poisoning are still common and are one of the causes of death and death in Indonesia. In 2019, there were 77 cases of food poisoning in Indonesia. A fast, accurate, and specific detection method is needed to detect poisoning. In previous studies, the optimum conditions and formulas for detecting these bacteria have been obtained using Real Time PCR. The results of previous studies are used as the basis for the development of a prototype detection kit. In this study, validation was carried out, which aimed to confirm the results of previous studies so that a reproducible and accurate product for the detection of Salmonella typhi bacteria could be obtained. The results of this study showed that fim-C primers for Salmonella typhi were amplified at 95 base pairs (bp) with an annealing temperature of 60ºC and a standard DNA concentration of 50 ng/µL. The results of the Real Time PCR confirmation test of Salmonella typhi bacteria at a pre-denaturation of 3 minutes with a concentration of 10 pmol obtained the Ct value according to thestandard with previous studies. The Ct value obtained was 13.96 for S. typhi bacteria. Based on the results obtained, it can be conclude that the condition validation stage for pure cultures was successfully carried out by producing consistentand reproducible data