Test for Viability of Ascaris lumbricoides Eggs

We are using the cultivatio methods usualy for viability test of the Ascaris lumbricoides eggs. We have found the optimal cultivation methods requires water and 2.5% of Formol water at 20-25°C

Development of functional human oral mucosal epithelial stem/progenitor cell sheets using a feeder-free and serum-free culture system for ocular surface reconstruction

Ocular surface reconstruction (OSR) using tissue-engineered cultivated oral mucosal epithelial cell sheets (COMECS) is a promising newly developed treatment for patients with severe ocular surface disease. Until now, this technique has used exogenic and undefined components such as mouse-derived 3T3 feeder cells and fetal bovine serum. To minimize associated risks of zoonotic infection or transmission of unknown pathogens and so establish a safe and effective protocol for the next generation of treatment modality, we developed a novel technique for the COMECS protocol, using a feeder-free and serum-free (FFSF) culture system. Following this new protocol, COMECS exhibited 4-5 layers of well-stratified and differentiated cells, and we successfully produced functional COMECS that included holoclone-type stem cells. Immunohistochemistry confirmed the presence of markers for cell junction (ZO1, Desmoplakin), basement membrane assembly (Collagen 7, Laminin 5), differentiation (K13, K3), proliferation (Ki67) and stem/progenitor cells (p75) in the FFSF COMECS. When transplanted to the ocular surfaces of rabbits, the tissue survived for up to 2 weeks. This study represents a first step toward assessing the development of functional FFSF COMECS for safe and ideal OSR

Imported malaria in Okayama prefecture

Two cases of imported malaria which occurred in Okayama prefecture are reported. One was infected with Plasmodium vivax in India, the other with P. falciparum at Nigeria, Africa. The efficacy of some antimalarial drugs in these cases is discussed. One patient was infected with P. falciparum, despite taking the medicine Daraprim® regularly. The efficacy of Daraprim® for suppressive cure in Nigeria is doutful. The therapy of chloroquine-resistance tropical malaria is also discussed

Imported malaria in Okayama prefecture

Two cases of imported malaria which occurred in Okayama prefecture are reported. One was infected with Plasmodium vivax in India, the other with P. falciparum at Nigeria, Africa. The efficacy of some antimalarial drugs in these cases is discussed. One patient was infected with P. falciparum, despite taking the medicine Daraprim® regularly. The efficacy of Daraprim® for suppressive cure in Nigeria is doutful. The therapy of chloroquine-resistance tropical malaria is also discussed