In vitro haemolytic, antioxidant and antibacterial (ESBLs and MRSA) activity of Datura metel L. flower and leaf extracts

500-506Prevalence of antibiotic resistant bacteria is an issue of concern as it affects the efficiency of antibiotics significantly. It makes the drug manufacturers continuously search for newer molecules that are safe and effective. To meet this challenge, researchers look towards natural resources, particularly plants, for possible new molecules that can be potentially tapped for medicinal purposes. In this context, here, we investigated Datura metel, commonly called the Angel’s trumphet as it is known for medicinal properties. We studied the hot water and methanol extracts of its flowers and leaves for antibacterial, antioxidant and antihemolytic activities. We used disc diffusion, phospho-molybdate and spectrophotometric methods. The bacteria tested were Methicillin resistant Staphylococcus aureus (MRSA) and β lactamase (ESBL) producing E. coli. The zone of inhibition (ZoI), as a measure of bacterial growth inhibition, was checked and found to range from 1.2-17.5 mm at the tested concentrations; ESBL producing E. coli was more subtle than MRSA. Total antioxidant capacity measured at 695 nm showed an average score of 17.4 μg/mL, displaying better activity of methanol extract. Antihemolytic property was tested on 0.5% RBC and percentage hemolysis was measured at optical density of 540 nm. Both the extracts showed considerably similar activity, though hot water lowered the hemolytic activity. Over all, the results suggest that D.metel could be exploited for its potential to inhibit drugresistant bacteria; free radical scavenging activity; and anti-hemolytic properties

In vitro haemolytic, antioxidant and antibacterial (ESBLs and MRSA) activity of Datura metel L. flower and leaf extracts

Prevalence of antibiotic resistant bacteria is an issue of concern as it affects the efficiency of antibiotics significantly. It makes the drug manufacturers continuously search for newer molecules that are safe and effective. To meet this challenge, researchers look towards natural resources, particularly plants, for possible new molecules that can be potentially tapped for medicinal purposes. In this context, here, we investigated Datura metel, commonly called the Angel’s trumphet as it is known for medicinal properties. We studied the hot water and methanol extracts of its flowers and leaves for antibacterial, antioxidant and antihemolytic activities. We used disc diffusion, phospho-molybdate and spectrophotometric methods. The bacteria tested were Methicillin resistant Staphylococcus aureus (MRSA) and β lactamase (ESBL) producing E. coli. The zone of inhibition (ZoI), as a measure of bacterial growth inhibition, was checked and found to range from 1.2-17.5 mm at the tested concentrations; ESBL producing E. coli was more subtle than MRSA. Total antioxidant capacity measured at 695 nm showed an average score of 17.4 μg/mL, displaying better activity of methanol extract. Antihemolytic property was tested on 0.5% RBC and percentage hemolysis was measured at optical density of 540 nm. Both the extracts showed considerably similar activity, though hot water lowered the hemolytic activity. Over all, the results suggest that D. metel could be exploited for its potential to inhibit drug resistant bacteria; free radical scavenging activity; and anti-hemolytic properties

A PILOT STUDY ON SUN PROTECTION FACTOR OF PLANT EXTRACTS: AN OBSERVATIONAL STUDY

Objective: The aim of the present work is to investigate the presence of ultraviolet (UV) light absorption capacity of the selected plant extracts. Chemical-based sunscreens provide protection against the sun's rays, in particular, UV radiation. Sometimes, exposure to these chemicals leads to allergies to the skin. The best way to protect skin from chemical and photoallergies is to use plant-based creams and lotions.Methods: Methanol and water extracts of Datura metel leaves and flowers; Loranthus leaves and flowers; and Eucalyptus leaves were prepared and checked their UV absorption ability from 200 nm to 450 nm. Different concentrations of the same extracts were tested for their sun protection factor (SPF) using Mansur formula from 290 to 320 nm. All the extracts showed absorption at UV range.Result: The calculated values of SPF were fallen in the range of 0.4–23.5. Among the three plants used in this study, methanol extract of Eucalyptus showed highest UV absorption than the other two plants. In vitro studies of a few phenolics from various plants have been found to be mutagenic. This might be the result of pro-oxidant than antioxidant action of these compounds. Keeping in view of human use as SPFs, it is indispensable to investigate the toxic effects of the extracts.Conclusion: The preliminary findings of this study reveal that the methanol extract of Eucalyptus has a high sun protection effect. This extract can be used as additives in other sunscreen formulations to enhance their SPF