2 research outputs found
Loss of MUC2 expression correlates with progression along the adenoma-carcinoma sequence pathway as well as de novo carcinogenesis in the colon
Aims: We have previously demonstrated
links between clinicopathological findings and
phenotypes using several gastric and intestinal
phenotypic markers in stomach and pancreatic cancers.
However, the clinicopathological significance of the
phenotype and Cdx2 expression has hitherto remained
unclear in colorectal carcinogenesis. Methods and
results: We examined the correlation between gastric and
intestinal phenotypic expression in 91 primary early
carcinomas of the colon. MUC2 expression
demonstrated a significant decrease from tubular/
tubulovillous adenomas with moderate atypia, through
intramucosal carcinomas, to cancers with submucosal
invasion (P<0.0001). Intramucosal de novo carcinomas
(flat type carcinomas without adenomatous components)
exhibited a greater decrease of MUC2 than intramucosal
lesions with adenomatous components. Expression of
MUC5AC also decreased significantly with progression
according to the tubular/tubulovillous adenomacarcinoma
sequence, carcinomas with villous
adenomatous components having a higher level
compared with their tubular adenomatous counterparts,
suggesting differences in the pathway of malignant
transformation. Cdx2 nuclear expression was maintained
in all of the adenomas and early carcinomas examined.
Conclusions: Our data suggest that the reduction of
MUC2 expression may be associated with the occurrence and progression of colorectal carcinomas in
both adenoma-carcinoma sequence pathway and de novo
carcinogenesis. Tumor-suppressive effects of Cdx2 may
be preserved during early stages of colorectal
carcinogenesis
Antiinflammatory therapy with canakinumab for atherosclerotic disease
BACKGROUND: Experimental and clinical data suggest that reducing inflammation without affecting lipid levels may reduce the risk of cardiovascular disease. Yet, the inflammatory hypothesis of atherothrombosis has remained unproved. METHODS: We conducted a randomized, double-blind trial of canakinumab, a therapeutic monoclonal antibody targeting interleukin-1β, involving 10,061 patients with previous myocardial infarction and a high-sensitivity C-reactive protein level of 2 mg or more per liter. The trial compared three doses of canakinumab (50 mg, 150 mg, and 300 mg, administered subcutaneously every 3 months) with placebo. The primary efficacy end point was nonfatal myocardial infarction, nonfatal stroke, or cardiovascular death. RESULTS: At 48 months, the median reduction from baseline in the high-sensitivity C-reactive protein level was 26 percentage points greater in the group that received the 50-mg dose of canakinumab, 37 percentage points greater in the 150-mg group, and 41 percentage points greater in the 300-mg group than in the placebo group. Canakinumab did not reduce lipid levels from baseline. At a median follow-up of 3.7 years, the incidence rate for the primary end point was 4.50 events per 100 person-years in the placebo group, 4.11 events per 100 person-years in the 50-mg group, 3.86 events per 100 person-years in the 150-mg group, and 3.90 events per 100 person-years in the 300-mg group. The hazard ratios as compared with placebo were as follows: in the 50-mg group, 0.93 (95% confidence interval [CI], 0.80 to 1.07; P=0.30); in the 150-mg group, 0.85 (95% CI, 0.74 to 0.98; P=0.021); and in the 300-mg group, 0.86 (95% CI, 0.75 to 0.99; P=0.031). The 150-mg dose, but not the other doses, met the prespecified multiplicity-adjusted threshold for statistical significance for the primary end point and the secondary end point that additionally included hospitalization for unstable angina that led to urgent revascularization (hazard ratio vs. placebo, 0.83; 95% CI, 0.73 to 0.95; P=0.005). Canakinumab was associated with a higher incidence of fatal infection than was placebo. There was no significant difference in all-cause mortality (hazard ratio for all canakinumab doses vs. placebo, 0.94; 95% CI, 0.83 to 1.06; P=0.31). CONCLUSIONS: Antiinflammatory therapy targeting the interleukin-1β innate immunity pathway with canakinumab at a dose of 150 mg every 3 months led to a significantly lower rate of recurrent cardiovascular events than placebo, independent of lipid-level lowering. Copyright © 2017 Massachusetts Medical Society