1 research outputs found
Ratiometric Two-Photon Fluorescent Probe for Quantitative Detection of β‑Galactosidase Activity in Senescent Cells
We reported a ratiometric two-photon
fluorescent probe (SG1) for
β-galactosidase (β-gal) and its application to quantitative
detection of β-gal activity during cellular senescence in live
cells and in aged tissues. This probe is characterized by a significant
two-photon excited fluorescence, a marked blue-to-yellow emission
color change in response to β-gal, easy loading, insensitivity
to pH and reactive oxygen species (ROS), high photostability, and
low cytotoxicity. In addition, we show that SG1 labeling is an effective
tool for quantitative detection of senescence-associated β-gal
activity at the subcellular level in situ. This finding demonstrates
that SG1 will find useful applications in biomedical research, including
studies of cell aging