Synergistic antinociceptive activity of combined aqueous extracts of <i>Artemisia campestris</i> and <i>Artemisia herba</i>-<i>alba</i> in several acute pain models

<p>In this study, total phenolic and flavonoid contents, acute toxicity and the antinociceptive activity of <i>Artemisia campestris</i> and <i>Artemisia herba</i>-<i>alba</i>, individually and in combination, were investigated using multiple forms of pain in animals. Our results have been shown that plants are relatively safe without clinical signs of toxicity in animals. Thus, extracts were presented high levels in phenolic and flavonoid contents. <i>Artemisia</i> decoctions with 100, 200, 400 mg/kg b-w studied dose, clearly attenuate chemical and thermal noxious stimuli in writhing, formalin and hot-plate tests, and significantly reduced paw oedema in formalin test. Additionally, binary combination forms exhibited a great improvement in intensity and amplitude of antinociceptive activity in comparison with both plants used individually by a relative interference with opioid system. Our findings suggested the central and peripheral analgesic properties and confirmed the folkloric medicinal use of these plants in pain symptom treatment.</p