A Non-Invasive method of quantifying pancreatic volume in mice using micro-MRI

In experimental models of pancreatic growth and recovery, changes in pancreatic size are assessed by euthanizing a large cohort of animals at varying time points and measuring organ mass. However, to ascertain this information in clinical practice, patients with pancreatic disorders routinely undergo non-invasive cross-sectional imaging of the pancreas using magnetic resonance imaging (MRI) or computed tomography (CT). The aim of the current study was to develop a thinsliced, optimized sequence protocol using a high field MRI to accurately calculate pancreatic volumes in the most common experimental animal, the mouse. Using a 7 Telsa Bruker micro-MRI system, we performed abdominal imaging in whole-fixed mice in three standard planes: axial, sagittal, and coronal. The contour of the pancreas was traced using Vitrea software and then transformed into a 3-dimensional (3D) reconstruction, from which volumetric measurements were calculated. Images were optimized using heart perfusion-fixation, T1 sequence analysis, and 0.2 to 0.4 mm thick slices. As proof of principle, increases in pancreatic volume among mice of different ages correlated tightly with increasing body weight. In summary, this is the first study to measure pancreatic volumes in mice, using a high field 7 Tesla micro-MRI and a thin-sliced, optimized sequence protocol. We anticipate that micro-MRI will improve the ability to non-invasively quantify changes in pancreatic size and will dramatically reduce the number of animals required to serially assess pancreatic growth and recovery.© 2014 Paredes et al

MRI sequence protocols and some of their key differences.

<p>TE, Echo Time; TR, Repetition Time. The matrix size for each of the sequences was 256X19.</p

Micro-MRI can accurately measure increases in pancreatic volume with advancing age.

<p>(A) Changes in pancreatic volume (in red) correlate with body weight (in blue). (n = 3 mice per age group). *, p<0.05, using a one way ANOVA. (B) Scatter plot demonstrating that pancreatic volume correlates tightly with body weight.</p

Method for generating a 3D reconstruction of the mouse pancreas.

<p>(A) Gross dissection of the pancreas with its adjoining organs. The pancreas was traced in each (B) axial image and, for further delineation, tracings were cross-checked, as necessary, using (C) sagittal and (D) coronal planes. (E) Three representative 3D reconstructions of the pancreas, generated using these tracings, are shown.</p

Preparation of the mouse for MRI.

<p>In these studies, whole-fixed mice were (A) placed in a conical tube and (B) inserted into a Bruker 7 Tesla micro-MRI. (C) Compared to immersion fixation, (D) <i>in vivo</i> perfusion fixation yielded a more homogenous pancreatic MRI signal (red outline).</p

Micro-MRI can accurately measure small volumes in phantom tubes.

<p>(A) Known volumes of gadolinium contrast, ranging from 50 to 200 mm³, with 25 mm³ increments, were loaded into small conical tubes (top row) and imaged using an optimized MRI protocol (bottom row). (B) There was a tight correlation between known and MRI-measured volumes.</p