Substantiation of rational relationships of main parameters of the rock washing-out process when applying GD-300 hydraulic monitors at Kuzbass open pits

The need to use hydraulic technology for washing out quaternary bedrock, as well as heterogeneous rocks previously piled in hydraulic-mine dumps located above commercial coal reserves, requires the use of modern high-performance equipment. As such equipment, it is advisable to use the new GD-300 hydraulic mining giants – monitors, which are designed, manufactured and tested at Kuzbass open pits. In order to increase the performance of hydraulic monitors at Kuzbass open pits, the following dependencies are determined in the article: changes in the amount of water flow through the hydraulic monitors on the nozzle diameter and the pressure in front of it; changes in the amount of solid rock washed out by the hydraulic monitor ‒ solid flow on the nozzle diameter at various values of the pressure in front of it; changes in the solid flow on the pressure in front of the nozzle

Refraction, fluorescence, and Raman spectroscopy of normal and glycated hemoglobin

In this study, the optical properties of glycated (HbA1c) and non-glycated (Hb) hemoglobin are compared using refractometry, fluorescence and Raman spectroscopy. The fluorescence measured at an excitation wavelength of 270 nm indicates differences in the molecular structure of hemoglobins. Analysis of the spectral shift of Raman spectra also showed variations indicating differences in their molecular structure. The refractive index measured in the visible and near IR regions for different temperatures allowed for quantification of mean values of temperature increment, which are quite different as dn/dT= –(1.03 ± 0.05)×10–4 °C–1 for Hb and – (1.37 ± 0.07)×10–4 °C–1for HbA1c.The data obtained in the work can serve as a basis for further study of the optical properties of glycated hemoglobin and other glycated proteins

Surface-enhanced Raman spectroscopy reveals structure complexity difference in single extrapulmonary Mycobacterium tuberculosis bacteria with different drug resistance

In this work, we argue that the SERS technique allows distinguishing between drug-sensitive and drug-resistant strains on the level of resolution of individual mycobacteria. This result is based on the experimental investigations of deactivated M. tuberculosis strains by the Raman spectroscopy in high-wavenumber region. A discussion of drug resistance-related complexity of emerging specific vibrational bands for proteins and lipids forming a cell’s wall is provided

Optical properties of glycated and non-glycated hemoglobin – Raman/fluorescence spectroscopy and refractometry

In this study, the optical properties of glycated (HbA1c) and nonglycated (Hb) hemoglobin are compared using Surface-enhanced Raman spectroscopy (SERS), spectrofluorimetry, and refractometry. Analysis of the spectral shift of SERS spectra showed good discrimination between two hemoglobins indicating differences in their molecular structure. The fluorescence spectra measured at excitation wavelengths of 260, 270, and 280 nm also indicate differences in the molecular structure of these hemoglobins. For the first time refractive index temperature increments were measured for HbA1c in a wide wavelength range in the visible and NIR as –(1.35 ± 0.11) ´ 10–4 °C–1 and compared with normal hemoglobin (dn/dT = –(1.02 ± 0.12) ´ 10–4 °C–1). The comparison of temperature RI increments for hemoglobin obtained from the whole blood of healthy volunteers and diabetic patients is also done. The data obtained can serve as a basis for further study of the optical properties of glycated hemoglobin and other glycated proteins