Amino acid substitutions found among the haplotypes of partial <i>cox1</i> gene.

<p>* No. of haplotypes.</p

Comparison of ITS-1 sequences of hookworms from apes and humans from Dzanga Sangha Protected Areas.

<p>Host and accession number in DNA database are given in parentheses. Dots indicate homologous nucleotides with <i>N. americanus</i> (<i>N. a.</i>) from Guatemala (AF217891); dash indicates absence of nucleotide. Major indels are shaded.</p

Comparison of ITS-2 sequences of hookworms from apes and humans from Dzanga Sangha Protected Areas.

<p>Host and accession number in DNA database are given in parentheses. Dots indicate homologous nucleotides with <i>N. americanus</i> (<i>N. a.</i>) from Guatemala (AF217891); dash indicates absence of nucleotide. Major indels are shaded.</p

Amplification of partial ITS-1 region using specific primers.

<p>Primers AmerF3 and AmerR2 only amplified specific band of about 250-1, while no clear band was formed for types II and III. CongF and Nem5.8R resulted in formation of specific band of about 650 bp for types II and III, while prominent bands were not formed for type I.</p

Restriction enzyme digestion of DNA sequences amplified with Civ18S1500F and Nem5.8R.

<p>By digestion with <i>Alu</i> I, two bands were formed for type I in area from 200 to 500 bp, while only one band was formed for types II and III. By digestion using <i>Hinc</i> II, two bands of about 300 and 500 bp were formed for type I, while only one band similar to original band was visible for types II and III.</p

Evolutionary relationships of <i>Necator</i> spp. from apes and humans in Dzanga-Sangha Protected Areas.

<p>Tree was reconstructed by Neighbor-Joining method on mtDNA <i>cox1</i> sequences each with 669 nucleotides. Host and accession number in DNA database in parentheses are given at each branch. Humans are CAR people except for two Europeans marked with asterisks, who acquired the infection in CAR. Bootstrap values larger than 50 are shown.</p

Map of study site in Dzanga-Sangha Protected Areas, Central African Republic.

<p>Dark grey – Dzanga and Ndoki Sectors of the protected Dzanga Ndoki National Park; light grey - Dzanga Sangha Dense Forest Special Reserve; 1 – location of villages Bayanga, Mossapoula and Yandumbé; M – research camp Mongambe; B – research camp Bai Hokou.</p