Ein-Schritt Expression und Immobilisierung von Enzymen in Zellhüllen aus Escherichia coli

Production of enzymes with combined immobilisation in one process step could reduce the costs for biocatalyst preparation. Therefore, enzymes were posttranslationally anchored to the cytoplasmic membrane of E. coli. The cells were subsequently opened by expression of lysis protein E from phage PhiX174 to obtain cellular envelopes with membrane anchored enzymes in stirred-tank bioreactor processes. Thus, active biocatalysts were obtained with up to 27,000 membrane-bound enzyme molecules.Die Herstellung von Enzymen mit Immobilisierung in einem Prozessschritt könnte die Produktionskosten von Biokatalysatoren reduzieren. Hierzu wurden Enzyme nach Expression in der Zytoplasmamembran von E. coli verankert. Danach wurden die Zellen durch Expression von Lyseprotein E aus dem Phagen PhiX174 geöffnet, um Zellhüllen mit immobilisierten Enzymen im Rührkesselreaktor zu erhalten. Dabei konnten bis zu 27.000 Enzymmoleküle pro Zellhülle verankert und aktive Biokatalysatoren erhalten werden

MOESM2 of A novel one-step expression and immobilization method for the production of biocatalytic preparations

Additional file 1: Figure S2. Comparison of β-galactosidase activities without detergent treatment. The activity of equal amounts of β-galactosidase before and after detergent treatment are compared