Quality of follicles (expressed as quantity and percentage of normal follicles) after in vitro and CAM-culture of cryopreserved ovarian pieces.

<p>Respective rates are not statistical different (P>0.1).</p

Cryopreserved ovarian medulla-free and medulla-containing pieces before and after 5 days culture with chorioallantoic membrane (CAM) system.

<p>(a, b, c, d, e) medulla-free piece, (a, b) just after thawing and seeding on CAM marked by silicone ring, (c, d, e) the same piece after culture, (c) piece on CAM, (d, e) piece in Petri dish; (f, g, h, i, j) medulla-containing piece, (f, g) just after thawing and seeding on CAM marked by silicone ring, (h, i, j) the same piece after culture, (c) piece on CAM, (d, e) piece in Petri dish; (e) outer CAM-layer with medulla-free piece, (j) inner CAM-layer with medulla-containing piece. Different intensiveness of the avian vascularisation in the place of the seeding of pieces was noted: (e) versus (j). Bar = 1 mm.</p

Vascularisation (von Willebrand factor expression) in cryopreserved medulla-containing ovarian tissue after in vitro and CAM-culture.

<p>Immonostained after in vitro (a, A) and CAM (b, B) culture. Bar = 0.25 mm.</p

Cryopreserved ovarian medulla-free and medulla-containing ovarian pieces after thawing and 8 days in vitro culture.

<p>(a, b) medulla-free piece, (a) just after thawing, (b) the same piece after culture, (c, d) medulla-containing piece, (c) just after thawing, (d) the same piece after culture. Bar = 1 mm.</p