Transition metal complexes with thiosemicarbazide-based ligands. LIII. [4-(2-diphenylphosphino-alpha-ethoxybenzyl-kappa P)-S-methyl-1-(4-oxidopent-3-en-4-ylidene-kappa O)-thiosemicarbazido-kappa(2) N-1, N-4]nickel (II)

The title compound, [Ni(C28H30N3O2PS)], crystallizes with two independent molecules in the asymmetric unit. The Ni-II atoms are in significantly deformed square-planar environments formed by an ONNP donor set from a thiosemicarbazide-based tetradentate ligand. The Ni-II atom and the ONN donor atoms are nearly coplanar, while the P atom deviates from their mean planes by 0.278 (4) and 0.202 (4) angstrom for the two independent molecules. The P-containing chelate rings are remarkably non-planar, adopting a boat conformation, which is unusual for chelate rings in transition metal complexes with thiosemicarbazide-based tetradentate ligands. The orientation of the ethoxy group bonded to this chelate ring is caused by an intramolecular C-H center dot center dot center dot pi interaction with the opposing phenyl ring. There are no hydrogen bonds; instead, numerous intermolecular C-H center dot center dot center dot pi interactions dominate in the crystal packing

Optimization of Submerged Cultivation Conditions for Extra- and Intracellular Polysaccharide Production by Medicinal Ling Zhi or Reishi Mushroom Ganoderma lucidum (W. Curt.: Fr.) P. Karst. (Aphyllophoromycetideae)

This study showed that cultivation conditions (medium composition, initial medium pH, aeration rate, inoculum density) affect biomass and polysaccharide production in Ganoderma lucidum strain HAI 447. The synthetic medium (pH 5.5) with maltose at the concentration of 65.0 g L-1, peptone of 0.2%, as well as in the presence of K, Na, and Mg at concentrations of 6.0, 3.0, and 2.0 mM, respectively, presented the best conditions for biomass (29.2 g L-1) and extra- and intracellular polysaccharide (2.9 mg mL(-1) and 73.3 mg g(-1), respectively) production in the analyzed strain. Constant aeration was optimal for biomass and extracellular polysaccharide production, whereas cultivation under stationary conditions was the best for intracellular polysaccharide production. A peak in biomass and polysaccharide production was observed at an inoculum density of 20.0 mL.Ministry of Science of Serbia [143041

Optimization of Submerged Cultivation Conditions for Extra- and Intracellular Polysaccharide Production by Medicinal Ling Zhi or Reishi Mushroom Ganoderma lucidum (W. Curt.: Fr.) P. Karst. (Aphyllophoromycetideae)

This study showed that cultivation conditions (medium composition, initial medium pH, aeration rate, inoculum density) affect biomass and polysaccharide production in Ganoderma lucidum strain HAI 447. The synthetic medium (pH 5.5) with maltose at the concentration of 65.0 g L-1, peptone of 0.2%, as well as in the presence of K, Na, and Mg at concentrations of 6.0, 3.0, and 2.0 mM, respectively, presented the best conditions for biomass (29.2 g L-1) and extra- and intracellular polysaccharide (2.9 mg mL(-1) and 73.3 mg g(-1), respectively) production in the analyzed strain. Constant aeration was optimal for biomass and extracellular polysaccharide production, whereas cultivation under stationary conditions was the best for intracellular polysaccharide production. A peak in biomass and polysaccharide production was observed at an inoculum density of 20.0 mL.Ministry of Science of Serbia [143041