Endüstriyel ölçekte üretilen Nar (Punica granatum L.) ve Maydanoz (Petroselinum crispum) tohumu yağının kimyasal bileşimi, antioksidan ve antifungal özelliklerinin belirlenmesi

In this study, essential oil and oil acid content, antioxidant and antifungal properties of oils obtained from pomegranate (Punica granatum L.) and parsley seeds (Petroselinium crispum) produced on an industrial scale were investigated. Pomegranate seed oil was obtained cold pressed in an industrial scale, while parsley seed oil was obtained industrial steam distillation. As a result of pomegranate seed oil GC-MS analysis, fifteen components were determined. Parsley seed oil was twelve compounds was identified. Punicic acid (61.19 %) was found as the dominant compound in pomegranate seed oil while apiole (14.21 %) was determined as the dominant compound in parsley seed oil. When the antioxidant capacity of the oils were examined, it was determined that the oils obtained from pomegranate seeds have a moderate antioxidant activity, the oils obtained from parsley seeds have high antioxidant activity. Antifungal activity of pomegranate (Punica granatum L.) and parsley (Petroselinium crispum) seed oil against five different plant pathogens, Fusarium. oxysporum f. sp. lycopersici, Botrytis cinerea, Sclerotinia sclerotiorum, Alternaria. solani and Rhizoctonia solani were also determined.Bu çalışmada endüstriyel ölçekte üretilmiş olan nar (Punica granatum L.) çekirdeği ve maydanoz (Petroselinium crispum) tohumundan elde edilen uçucu yağların ve yağ asitlerinin, antioksidan ve antifungal özellikleri incelenmiştir. Nar çekirdeği yağı endüstriyel soğuk press yöntemi ile üretilmiş iken, maydanoz tohumu yağı da endüstriyel buhar destilasyonu yöntemi ile elde edilmiştir. Nar çekirdeği yağında GC-MS analizi sonucunda 15 bileşen tespit edilmiştir. Maydanoz tohumu yağı içinde 12 bileşen tespit edilmiştir. Nar çekirdeği yağında pukinik asit (% 61.19) ana bileşen olarak bulunmuş iken, maydanoz çekirdeği yağında apiyol (% 14.21) ana bileşen olarak tespit edilmiştir. Yağların antioksidan aktivitesi incelendiğinde nar çekirdeğinden elde edilen yağlarda orta miktarda, maydanoz tohumlarından elde edilen yağlarda ise yüksek miktarda antioksidan aktiviteye sahip oldukları tespit edilmiştir. Ayrıca nar (Punica granatum L.) ve maydanoz (Petroselinium crispum) tohumu yağının beş farklı bitki patojenine Fusarium. oxysporum f. sp. lycopersici, Botrytis cinerea, Sclerotinia. sclerotiorum, Alternaria. solani ve Rhizoctonia solani antifungal aktivitesi olduğu tespit edilmiştir

Are elevated mitochondrial DNA fragments in prostatic inflammation a potential biomarker for prostate cancer?

Background: We sought to determine whether two soluble forms with different size of mtDNA are linked to prostatic inflammation, and whether they discriminate prostate cancer (PCa) from inflammatory prostatic conditions. Methods: Histopathologically diagnosed prostatitis, PCa and benign prostatic hyperplasia patients (n = 93) were enrolled in this study and they were categorized as with and without prostate inflammation. Quantitative RT-PCR was used to analyze the levels of 79-bp and 230-bp fragments in urine and blood samples collected following prostate massage. Results: The urine mtDNA-79 and mtDNA-230 were significantly increased in patients with prostate inflammation compared with those in without inflammation. Here, 79-bp fragment of apoptotic origin was significantly higher level than 230-bp fragment of necrotic origin. Although mtDNA-79 copy number in serum samples was also increased in patients with prostate inflammation, mtDNA-230 was similar in the two groups. Furthermore, mtDNA-79 and mtDNA-230 copy numbers in postprostate massage urine were higher (about 16-fold and 22-fold, respectively) than those from serum samples. ROC analysis showed that, although post-prostate massage urine have relatively higher performance than blood, ability to discriminate cases of both fragments was not better than that of serum total PSA. Conclusions: Our results demonstrate that shorter cf-mtDNA fragment size in particular, increase in the presence of prostate inflammation in post-prostatic massage urine but both fragments could never improve serum total PSA performance

Monitoring of platelet function parameters and microRNA expression levels in patients with prostate cancer treated with volumetric modulated arc radiotherapy

Radiotherapy (RT) may result in platelet activation and thrombosis development. To the best of our knowledge, the potential effect of volumetric-modulated arc therapy (VMAT), a novel radiotherapy technique, on platelet function and microRNA (miRNA/miR) expression has not been previously investigated. The present study aimed to determine the effect of VMAT on the alterations in platelet function parameters and miRNA expression levels. A total of 25 patients with prostate cancer and 25 healthy subjects were included in the present study. Blood samples were collected from the patient group on the day prior to RT (pre-RT), the day RT was completed (post-RT day 0), and 40 days following the end of therapy (post-RT day 40). Platelet count, mean platelet volume (MPV) value, platelet aggregation, plasma P-selectin, thrombospondin-1, platelet factor 4, plasma miR-223 and miR-126 expression levels were measured. A significant decrease in platelet count in the post-RT day 0 group was measured in comparison with the pre-RT and the post-RT day 40 groups. Pre-RT MPV values were higher than those of the post-RT day 0 and the post-RT day 40 groups. No significant differences were observed in the levels of platelet activation markers or miR-223 and miR-126 expression levels between the RT groups. Although RT may result in a reduction in platelet and MPV counts, the results of the present study indicate that platelet activation markers are not affected by VMAT. Therefore, it is possible that no platelet activation occurs during VMAT, owing to the conformal dose distributions, improved target volume coverage and the sparing of normal tissues from undesired radiation