Variability assessment of 14 SSR loci of <i>Chamelea gallina</i>.

<p>Variability, expressed in terms of number of different alleles, was assessed on 12 individuals collected in Chioggia in 2010 (off Venice lagoon, Italy). The table reports the name of each locus, taken from the contig number, the repeat content, the forward (F) and reverse (R) primer sequences, the fluorescent label, the annealing temperature (Ta) of PCR amplification, the size range of amplified fragments in bp, the allelic range in repeats, the number of alleles (Na) detected and the Hardy-Weinberg probability (pHWE). Significant p-values in bold (α = 0.05). Mean values for allele number, observed and expected heterozygosity are reported in the last row. Standard Deviation is reported in brackets (± SD).</p>*<p>Loci putatively affected by null alleles following MICRO-CHECKER 2.2.3. <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0044185#pone.0044185-vanOosterhout1" target="_blank">[51]</a>.</p>a<p>p-values were calculated based on a limited number of individuals (n = 12).</p

Set of annotated contigs of <i>Chamelea gallina</i> trascriptome with nucleotide and protein BLAST hits.

<p>The Venn diagram reports the intersection between number of contigs with BLAST hits in nr protein and nt nucleotide databases.</p

Summary of SSR types and frequency in <i>Chamelea gallina</i> transcriptome.

<p>Summary of SSR types and frequency in <i>Chamelea gallina</i> transcriptome.</p

<i>Chamelea gallina</i> SSR mining results.

<p>The table reports the main results provided by MISA for SSR detection.</p

Length and quality of <i>Chamelea gallina</i> transcriptome contigs.

<p>Panel histograms report the contig's length (a) and average quality (b) distribution, while panel c shows the relationship between length and quality.</p

Putative SNPs identified from <i>Chamelea gallina</i> transcriptome database.

<p>More than 20,000 SNPs were identified out of 36,422 contigs and meta-contigs.</p

ChameleaBase.

<p>The screenshots report the <i>Chamelea gallina</i> database online version homepage (on the upper right side), the search facility (bottom right) and an example of the gene-like entry (on the left).</p