Kainic acid induces expression of caveolin-1 in activated microglia in rat brain

Caveolin-1, a major constituent of caveolae, has been implicated in endocytosis, signal transduction and cholesterol transport in a wide variety of cells. In the present study, the expression of caveolin-1 was examined by immunohistochemistry in rat brain with or without systemic injection of kainic acid (KA). Caveolin-1 immunoreactivity was observed in capillary walls in brains of control rats. From one to seven days after KA injection, caveolin-1 immunoreactivity appeared in activated microglia in the cerebral cortex, hippocampus and other brain regions. The strongest immunoreactivity of microglia was seen after 3 days after KA administration. The expression of caveolin-1 was confirmed by RT-PCR and Western blot analysis, respectively. The induction of caveolin-1 expression in microglia activated in response to kainic acid administration suggests its possible role in a modulation of inflammation. (Folia Histochemica et Cytobiologica 2013, Vol. 51, No. 1, 25–30

Comprehensive behavioral phenotyping of a new Semaphorin 3 F mutant mouse

Background: Semaphorin 3 F (Sema3F) is a secreted type of the Semaphorin family of axon guidance molecules. Sema3F and its receptor neuropilin-2 (Npn-2) are expressed in a mutually exclusive manner in the embryonic mouse brain regions including olfactory bulb, hippocampus, and cerebral cortex. Sema3F is thought to have physiological functions in the formation of neuronal circuitry and its refinement. However, functional roles of Sema3F in the brain remain to be clarified. Here, we examined behavioral effects of Sema3F deficiency through a comprehensive behavioral test battery in Sema3F knockout (KO) male mice to understand the possible functions of Sema3F in the brain. Results: Male Sema3F KO and wild-type (WT) control mice were subjected to a battery of behavioral tests, including neurological screen, rotarod, hot plate, prepulse inhibition, light/dark transition, open field, elevated plus maze, social interaction, Porsolt forced swim, tail suspension, Barnes maze, and fear conditioning tests. In the open field test, Sema3F KO mice traveled shorter distance and spent less time in the center of the field than WT controls during the early testing period. In the light/dark transition test, Sema3F KO mice also exhibited decreased distance traveled, fewer number of transitions, and longer latency to enter the light chamber compared with WT mice. In addition, Sema3F KO mice traveled shorter distance than WT mice in the elevated plus maze test, although there were no differences between genotypes in open arm entries and time spent in open arms. Similarly, Sema3F KO mice showed decreased distance traveled in the social interaction test. Sema3F KO mice displayed reduced immobility in the Porsolt forced swim test whereas there was no difference in immobility between genotypes in the tail suspension test. In the fear conditioning test, Sema3F KO mice exhibited increased freezing behavior when exposed to a conditioning context and an altered context in absence of a conditioned stimulus. In the tests for assessing motor function, pain sensitivity, startle response to an acoustic stimulus, sensorimotor gating, or spatial reference memory, there were no significant behavioral differences between Sema3F KO and WT mice. Conclusions: These results suggest that Sema3F deficiency induces decreased locomotor activity and possibly abnormal anxiety-related behaviors and also enhances contextual memory and generalized fear in mice. Thus, our findings suggest that Sema3F plays important roles in the development of neuronal circuitry underlying the regulation of some aspects of anxiety and fear responses

Pharmacological treatment for bipolar mania: a systematic review and network meta-analysis of double-blind randomized controlled trials

A systematic review and random-effects model network meta-analysis was conducted to compare the efficacy, acceptability, tolerability, and safety of pharmacological interventions for adults with acute bipolar mania. We searched PubMed, the Cochrane Library, and Embase databases for eligible studies published before March 14, 2021. Randomized controlled trials (RCTs) of oral medication monotherapy lasting ≥10 days in adults with mania were included, and studies that allowed the use of antipsychotics as a rescue medication during a trial were excluded. The primary outcomes were response to treatment (efficacy) and all-cause discontinuation (acceptability). The secondary outcomes were the improvement of mania symptoms and discontinuation due to inefficacy. Of the 79 eligible RCTs, 72 double-blind RCTs of 23 drugs and a placebo were included in the meta-analysis (mean study duration = 3.96 ± 2.39 weeks, n = 16442, mean age = 39.55 years, with 50.93% males). Compared with the placebo, aripiprazole, asenapine, carbamazepine, cariprazine, haloperidol, lithium, olanzapine, paliperidone, quetiapine, risperidone, tamoxifen, valproate, and ziprasidone outperformed response to treatment (N = 56, n = 14503); aripiprazole, olanzapine, quetiapine, and risperidone had lower all-cause discontinuation; however, topiramate had higher all-cause discontinuation (N = 70, n = 16324). Compared with the placebo, aripiprazole, asenapine, carbamazepine, cariprazine, haloperidol, lithium, olanzapine, paliperidone, quetiapine, risperidone, tamoxifen, valproate, and ziprasidone outperformed the improvement of mania symptoms (N = 61, n = 15466), and aripiprazole, asenapine, carbamazepine, cariprazine, haloperidol, lithium, olanzapine, paliperidone, quetiapine, risperidone, valproate, and ziprasidone had lower discontinuation due to inefficacy (N = 50, n = 14284). In conclusions, these antipsychotics, carbamazepine, lithium, tamoxifen, and valproate were effective for acute mania. However, only aripiprazole, olanzapine, quetiapine, and risperidone had better acceptability than the placebo

トラウマ タイケン ノ ウム ト ソノ コウゾウカ カイジ ガ シンシン キノウ ニ オヨボス エイキョウ

The purposes of this study were to compare health, cognitive functioning, and negative rumination between individuals who have experienced trauma and those who do not, and to examine the effect of structured disclosure of trauma on posttraumatic stress reactions (PTSR), health, and cognitive functioning. In Study Ⅰ, participants were 24 undergraduates at 9 or more scores of Impact of Event Scale(IES), which measures PTSR(the trauma group), and 15 undergraduates with no trauma (the no trauma group). Results showed that the trauma group was more unhealthy and ruminative than the no trauma group. In Study Ⅱ, participants in the trauma group were randomly assigned into three groups: the structured disclosure group(n=9), the free disclosure group(n=8), and the control group(n=7). Participants in the structured disclosure group were asked to write adaptive thought of the event. The free disclosure group wrote the deepest emotion and thought about the trauma freely. The control group wrote their plan after the experiment without emotion. Results showed that all the participants in the groups revealed significantly improvement in PTSR, mental health, and cognitive functioning from pre assessment to 1 month follow-up assessment. Results of this study were discussed in connection with previous studies

トラウマ ノ コウゾウカ カイジ ガ シンシン キノウ ニ オヨボス エイキョウ : ガイショウゴ セイチョウ ノ ケントウ

The purpose of this study was to examine effects of the structured disclosure of trauma on physical and mental function, and posttraumatic growth (PTG). In this study, we revised structured disclosure to promote confrontation to traumatic memory. Participants were 26 undergraduates at 9 or more scores of Impact of Event Scale (IES) which measures posttraumatic stress reactions (PTSR). They were randomly assigned into three groups: the structured disclosure group (n=10), the free disclosure group (n=6), and the control group (n=10). Participants in the structured disclosure group were asked to write adaptive thought of the event. The free disclosure group wrote the deepest emotion and thought about the trauma freely. The control group wrote their plan after the experiment without emotion. All the participants were asked to write for 20 minutes on 3 days. Results showed that all groups revealed significantly improvement in PTSR from pre assessment to 3 month follow-up assessment. Although the biggest effect size (ES ) was calculated in the structured disclosure group, significant difference with the control group was not found. Though neither significant main effect nor interaction was found in PTG, result of comparison of the ES of PTG showed that the free disclosure group had a medium ES . It is suggested that confrontation and cognitive reappraisal are necessary together in written disclosure

トラウマ ニ カンレン スル ハンスウ ト シンニュウ シコウ ノ カンレン オヨビ デキゴト カラ ノ キョリカ オ ソクシン スル ヨウ コウゾウカ サレタ ヒッキ ノ コウカ ノ ケントウ

The purposes of this study were to examine the relationship between trauma-related rumination and intrusive thinking, and the effects of structured disclosure to facilitate distancing from the event on the trauma-related rumination, posttraumatic stress reactions (PTSR). In Study I, 38 undergraduates who maintain 9 or more scores of Impact of Event Scale (IES) for one month responded broad-trauma related rumination scale (BTRRS) and IES two times. Regression analysis revealed that there was association with the scores on changes of both factors of BTRRS (broad trauma-related rumination and degree of distancing from the broad trauma) and the scores on changes of intrusion factor of IES（R2=.162，p=.007；R2=.253，p=.001）. In Study II, participants in the Study I were randomly assigned into three groups: the structured disclosure group(n=8), the free disclosure group(n=7) and the control group(n=8). The structured disclosure group was asked to write the event to facilitate distancing from it. In particular, they wrote the event, and after that wrote it again from the other person's perspective. The free disclosure group wrote the event and the event-related emotion freely. The control group wrote their behavior on the day before and before experiment, and the plan after experiment without emotion. Results showed that the structured disclosure increased short-term distancing more than other groups, but did not increase long-term one and decrease the rumination. Although all participants in groups decreased PTSR and the distress about the event after one month of disclosure, the differences on groups were not significant. Results of this study were discussed from the theoretical standpoints

Measurements of Cosmic-ray Low-energy Antiproton and Proton Spectra in a Transient Period of the Solar Field Reversal

The energy spectra of cosmic-ray low-energy antiprotons and protons have been measured by BESS in 1999 and 2000, during a period covering the solar magnetic field reversal. Based on these measurements, a sudden increase of the antiproton to proton flux ratio following the solar magnetic field reversal was observed, and it generally agrees with a drift model of the solar modulation.Comment: 4 pages, 4 figures, revised version accepted for publication in Phys. Rev. Let

Oxygen isotope evidence from Ryugu samples for early water delivery to Earth by CI chondrites

The delivery of water to the inner Solar System, including Earth, is still a debated topic. A preferential role for hydrated asteroids in this process is supported by isotopic measurements. Carbonaceous chondrite (CC) meteorites represent our main source of information about these volatile-rich asteroids. However, the destruction of weaker materials during atmospheric entry creates a bias in our CC data. The return of surface materials from the C-type asteroid 162173 Ryugu by the Hayabusa2 spacecraft provides a unique opportunity to study high-porosity, low-density, primitive materials, unrepresented in the meteorite record. We measured the bulk oxygen isotope composition from four Ryugu particles and show that they most closely resemble the rare CI (CC Ivuna-type) chondrites, but with some differences that we attribute to the terrestrial contamination of the CI meteorites. We suggest that CI-related material is widespread among carbonaceous asteroids and a more important source of Earth’s water and other volatiles than its limited presence in our meteoritic collection indicates