Primer list for real-time PCR.

<p>Primer list for real-time PCR.</p

Effects of the SNP rs536714306 on <i>GPR126</i> signal transduction in HEK293T cells.

<p>(A) HEK293T cells were transfected with empty vector, wild-type GPR126, or mutant GPR126 containing SNP rs536714306 for 24 h, and expression of GPR126 and β-Actin was assessed by western blotting. Asterisk represents a non-specific band. (B) HEK293T cells were transfected with empty vector, wild-type GPR126, or mutant GPR126 for 48 h. Following a 1 h incubation with 3 μg/mL type IV collagen, cAMP expression was assessed by ELISA. Data represent the average and standard deviation of three independent experiments. *<i>p</i> <0.05. EV, empty vector; WT, wild-type GPR126; mut, mutant GPR126.</p

Expression of GPR126 during the cytodifferentiation of HPDL cells.

<p>HPDL cells were transfected with empty vector and incubated with mineralization medium for 4, 6, 9, and 15 days and the expression of BSP and GPR126 was assessed by real-time PCR. Data represent the average and standard deviation of four independent experiments. *<i>p</i> <0.05.</p

List of 10 filtered variants.

<p>List of 10 filtered variants.</p

GWAS findings.

<p>GWAS findings.</p

Clinical characteristic of individual subjects in the GWAS.

<p>Clinical characteristic of individual subjects in the GWAS.</p