Analysis of deletion polymorphism of xenobiotics detoxication system genes in patients with tuberculosis and diabetes mellitus

An analysis of the occurrence of alleles and genotypes of GSTM1 gene in patients with pulmonary tuberculosis and diabetes mellitus regarding the MBT resistance version allowed to establish that under the conditions of pulmonary tuberculosis infection GSTM1 gene deletion mutation can be found in one out of five (21.87% of cases), and the occurrence due to the MBT resistance version is: with NDTB - 17.39%, with MDR-TB 35.0%  and - PRTB 20.0% respectively. According to the nature of the distribution of allelic gene GSTM1 a favorable functional 1 allele prevails (73.29%) in the normal inbreeding among patients and deficiency of heterozygosity among healthy people, which generally forms a normal population distribution for the European race. Material and methods. The study involved 100 patients with newly diagnosed pulmonary TB and diabetes mellitus who had been hospitalized in Chernivtsi Regional TB Dispensary. The control group consisted of 50 healthy individuals. Genomic DNA was isolated from the whole venous blood. GSTM1 polymorphic areas were isolated by means of multicomplex polymerase chain reaction, according to the protocol for instantaneous analysis of polymorphism by M. Arana et all (1996). Deletion of gene corresponds to the lack of appropriate strips in the electropherogram. Results and discussion. Despite the fact that the activity of the enzyme glutathione-S-transferase of class M is encoded by five GST genes of class M (M1-M5), the dominant cause of genetically caused dysregulation of antioxidant activity is deletion (null) polymorphism of the gene GSTM1. Conclusion. Among the patients with pulmonary tuberculosis and diabetes mellitus one out of five persons (21,87 % of cases) was diagnosed with deletion mutation of GSTM1 gene; and the occurrence due to MBT resistance variation is: in NDTB-17,39 %, in MDR-TB - 35,0 % and in PRTB- 20,0 % respectively

Использование сиропа изониазида для профилактики туберкулеза у детей

Utilizarea izoniazidei sirop pentru prevenirea tuberculozei la copiiThe purpose of the study was to conduct a comparative analysis of the use of isoniazid in the form of syrup and tablet for the prevention of tuberculosis in children. Materials and methods. 114 children at risk who were on sanatorium treatment at Chernivtsi children’s sanatorium (Ukraine) were examined for tuberculosis. For chemoprophylaxis in children the drug “Isoniazid” was used: 61 children received the drug in the form of syrup (group 1), 53 - in tablet form (group 2). On the basis of Institute of Pneumology “Marius Nasta” (Romania) 83 children enrolled to the 1 group received preventive therapy with isoniazid in the form of syrup. Results. Significantly lower levels of total protein were reported in 1 group - by 9.6% than in patients in 2 group (р<0.05). Also significantly higher levels of bilirubin - by 17.2 %, ALT (alanine transaminase) - by 19 %, AST (aspartate transaminase) - by 22.4 % and thymol test - by 15.5 % were reported in 2 group compared with 1 group (p <0.05), evidencing that there are preconditions for the development of toxic hepatitis when using isoniazid in tablet form. It is well established that the changes in blood chemistry were much more pronounced in children who received isoniazid tablets and were increasing up to the completion of the course of chemoprophylaxis. Conclusions. The results of complex clinical, laboratory and instrumental study allow us to recommend “Isoniazid” in the form of syrup 100 mg / 5 ml as highly effective drug for the prevention of tuberculosis in children.Использование сиропа изониазида для профилактики туберкулеза у дете

Abstracts of the First Eurasian Conference; The Coronavirus Pandemic and Critical ICT Infrastructure

While the world is struggling with COVID-19, the ICT industry seeks to play a constructive role in combating the spread of the virus. This book contains the abstracts of the papers presented at The First Eurasian Conference; The Coronavirus Pandemic and Critical ICT Infrastructure (PANDEMIC-ICT-2020) organized by AMIR Technical Services LLC, Tbilisi, Georgia held on November 28-30, 2020. Conference Title: The Coronavirus Pandemic and Critical ICT InfrastructureConference Acronym: PANDEMIC-ICT-2020Conference Date: 28-30 November 2020Conference Location: Online (Virtual Mode)Conference Organizer: AMIR Technical Services LLC, Tbilisi, Georgi