Insertion strategies used with lone English verbs in otherwise Igbo utterances

Using examples of tape recorded conversational data from fifty educated adult Igbo-English bilinguals resident in Port Harcourt, Nigeria, this paper demonstrates that lone English verbs are typically inserted into otherwise Igbo utterances by means of Igbo verbal inflectional morphology. Other verbs are adjoined to a helping verb from Igbo, specifically involving an adapted form. Yet a few English verbs are inserted into a position corresponding to an Igbo verb without any adaptations. To answer the question as to why the verbal inflectional morphology of Igbo rather than that of English should be used, we show that this is predicted by the Matrix Language Frame (MLF) model, according to which integration into the bound morphology of the base language is expected. Also, the paper identifies that the un-adapted English verbs occur in Igbo serial verb constructions (SVCs). The only type of structure where a full Igbo verb may occur without verbal morphology. Consequently, this paper concludes by arguing that the un-adapted English verbs in Igbo SVCs do not occur in codeswitching (CS) because of the activation of a ‘CS-specific’ compromise strategy, rather they, like the English verbs bearing Igbo verbal inflectional morphology occur in clause structure with restrictions imposed by the base language grammar

Field testing and exploitation of genetically modified cassava with low-amylose or amylose-free starch in Indonesia

The development and testing in the field of genetically modified -so called- orphan crops like cassava in tropical countries is still in its infancy, despite the fact that cassava is not only used for food and feed but is also an important industrial crop. As traditional breeding of cassava is difficult (allodiploid, vegetatively propagated, outbreeding species) it is an ideal crop for improvement through genetic modification. We here report on the results of production and field testing of genetically modified low-amylose transformants of commercial cassava variety Adira4 in Indonesia. Twenty four transformants were produced and selected in the Netherlands based on phenotypic and molecular analyses. Nodal cuttings of these plants were sent to Indonesia where they were grown under biosafety conditions. After two screenhouse tests 15 transformants remained for a field trial. The tuberous root yield of 10 transformants was not significantly different from the control. Starch from transformants in which amylose was very low or absent showed all physical and rheological properties as expected from amylose-free cassava starch. The improved functionality of the starch was shown for an adipate acetate starch which was made into a tomato sauce. This is the first account of a field trial with transgenic cassava which shows that by using genetic modification it is possible to obtain low-amylose cassava plants with commercial potential with good root yield and starch quality

Overexpression of Hydroxynitrile Lyase in Cassava Roots Elevates Protein and Free Amino Acids while Reducing Residual Cyanogen Levels

Cassava is the major source of calories for more than 250 million Sub-Saharan Africans, however, it has the lowest protein-to-energy ratio of any major staple food crop in the world. A cassava-based diet provides less than 30% of the minimum daily requirement for protein. Moreover, both leaves and roots contain potentially toxic levels of cyanogenic glucosides. The major cyanogen in cassava is linamarin which is stored in the vacuole. Upon tissue disruption linamarin is deglycosylated by the apolplastic enzyme, linamarase, producing acetone cyanohydrin. Acetone cyanohydrin can spontaneously decompose at pHs >5.0 or temperatures >35°C, or is enzymatically broken down by hydroxynitrile lyase (HNL) to produce acetone and free cyanide which is then volatilized. Unlike leaves, cassava roots have little HNL activity. The lack of HNL activity in roots is associated with the accumulation of potentially toxic levels of acetone cyanohydrin in poorly processed roots. We hypothesized that the over-expression of HNL in cassava roots under the control of a root-specific, patatin promoter would not only accelerate cyanogenesis during food processing, resulting in a safer food product, but lead to increased root protein levels since HNL is sequestered in the cell wall. Transgenic lines expressing a patatin-driven HNL gene construct exhibited a 2–20 fold increase in relative HNL mRNA levels in roots when compared with wild type resulting in a threefold increase in total root protein in 7 month old plants. After food processing, HNL overexpressing lines had substantially reduced acetone cyanohydrin and cyanide levels in roots relative to wild-type roots. Furthermore, steady state linamarin levels in intact tissues were reduced by 80% in transgenic cassava roots. These results suggest that enhanced linamarin metabolism contributed to the elevated root protein levels

Code-switching practices in Port Harcourt: code-switching as a linguistic resource

Code-switching is regarded by most researchers as an intricate and complex mode of discourse, one with several rules that govern its use. These regulations are not only grammatical; but social, and linguists have started to explore the depths of this phenomenon and its implications, not only for the study of bilingualism, but also to further our understanding of social and communicative interactions among various sets of people. This paper aims to catalogue and present some instances of how Port Harcourt Ikwerre-Nigerian Pidgin English (NPE) bilinguals employ code-switching as a linguistic resource for realizing particular communicative goals