E2F2 gene deletion does not promote changes in total triacylglycerol, phospholipid and protein content in quiescent liver or at 48-h after 70% partial hepatectomy.

<p>Partial hepatectomy was performed on E2F2^+/+ (wild-type, WT) and E2F2^-/- mice, and were sacrificed 48 hours later. Quiescent (0-h, solid bars) and regenerating (48-h, open bars) livers were harvested and homogenized and the triacylglycerol (TAG), phospholipid (PL) and protein content of homogenates were quantified as described in Materials and Methods. Data are shown as means ± SD and are representative of three independent experiments using cohorts of 6 WT and 6 E2F2^-/- mice each. Statistical differences between the regenerating and the quiescent tissue of a genotype are denoted by * <i>P</i>≤0.05, *** <i>P</i>≤0.001 (unpaired, 2-tailed Student's <i>t</i>-test).</p

E2F2 gene deletion alters the phospholipid composition of quiescent liver.

<p>Partial hepatectomy was performed on E2F2^+/+ (wild-type, WT) and E2F2^-/- mice, and were sacrificed 48 hours later. Quiescent (0-h, solid bars) and regenerating (48-h, open bars) livers were harvested and homogenized and lipids extracted from homogenates. The composition of the major phospholipid classes phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylinositol (PI), phosphatidylserine (PS), sphingomyelin (SM), cardiolipin (diphosphatidylglycerol, DPG), lysophosphatidylcholine (LPC) and phosphatidic acid (PA) were determined by phosphorous analysis after their separation by thin-layer-chromatography. Results are shown as mol% of total phosphorous. Data and presented as means ± SD and are representative of three independent experiments using cohorts of 6 WT and 6 E2F2^-/- mice each. Statistical differences between the regenerating and the quiescent liver of a genotype are denoted by * <i>P</i>≤0.05, ** <i>P</i>≤0.01, and between the same tissue condition of different genotypes are denoted by # <i>P</i>≤0.05 (unpaired, 2-tailed Student's <i>t</i>-test).</p

Fatty acid composition of triacylglycerols in wild-type (WT) and E2F2^-/- mice liver before (0-h) and 48 hours (48-h) post-partial hepatectomy.

<p>The results are expressed as a percentage of the fatty acids measured in the corresponding triacylglycerol sample and are the average ± SD of two independent experiments using cohorts of 6 E2F2^+/+ (wild-type, WT) and 6 E2F2^-/- mice each. SFA, saturated fatty acids; MUFA, monounsaturated fatty acids; PUFA, polyunsaturated fatty acids. Student's t-test for paired data: *<i>P</i>≤0.05 for comparisons of 48-h <i>vs</i> 0-h within the same genotype; # <i>P</i>≤0.05 and ## <i>P</i>≤0.01 for comparisons of the same condition between E2F2^-/- and WT groups.</p><p>Fatty acid composition of triacylglycerols in wild-type (WT) and E2F2^-/- mice liver before (0-h) and 48 hours (48-h) post-partial hepatectomy.</p