Sexual dimorphism in some psycho-neuro-endocrine parameters at human

Background. Despite the well-documented relationships between HRV, EEG and anxiety parameters, studies of their sexual dimorphism, judging by the results of the meta-analysis, have so far been conducted separately. The purpose of this study is to clarify sexual dimorphism in almost synchronously registered psycho-neuro-endocrine parameters. Materials and Methods. The object of observation were practically healthy volunteers: 30 females (30÷76 yr) and 31 males (24÷69 yr). In basal conditions we recorded the ongoing HRV and EEG, determined serum levels of testosterone, calcitonin, cortisol, aldosterone, and triiodothyronine, estimated the severity of the trait and reactive anxiety. After 4 or 7 days, repeated testing was performed. Results. Regardless of age, females differ significantly from males, except for drastically lower levels of testosterone and calcitonin by definition (but not cortisol, aldosterone and triiodothyronine), lower levels of HRV-markers of sympathetic tone (but not heart rate), reactive anxiety, and beta-rhythm asymmetry. On the other hand, trait anxiety, levels of HRV-markers of vagal tone, variability and amplitude of the beta-rhythm, and its power spectral density (PSD) in 12 loci (maximum differences in T6, F3, and T3 loci), amplitude of the theta-rhythm and its PSD in 16 loci (maximum differences in F3, C3, and T3 loci), PSD of the alpha-rhythm in T3, T6, F7, and T4 loci as well as entropy of PSD in F7 and F8 loci are significantly higher in females than in males. The listed parameters are determined by testosterone for 31,7%, and calcitonin for 26,3%. The method of discriminant analysis revealed 20 EEG and 6 HRV parameters as well as trait and reactive anxiety, based on the totality of which males can be retrospectively recognized (without testosterone and calcitonin) with an accuracy of 90,3%, and females with an accuracy of 88,3%. Conclusion. The revealed differences between the sexes were not influenced by at least two bioactive factors, that is, they are robust sex markers

Relationships between changes in the state of neuroendocrine regulation and immunity in patients of Truskavets’ spa

Background. Earlier we showed that the balneotherapeutic complex of Truskavets’ spa have significant modulating effects on parameters of EEG&HRV and Immunity of patients with chronic pyelonephritis and cholecystitis in remission accompanied of neuroendocrine-immune complex dysfunction. Were detected the close relationships between EEG, HRV, endocrine and immune parameters as well as between changes in EEG&HRV and immune parameters under the influence of balneotherapy. The aim of this study was to analyze the relationships between changes in the state of neuroendocrine regulation and immunity of the same patients. Materials and Methods. The object of clinical-physiological observation were 22 men aged 23-70 years, who underwent rehabilitation treatment of chronic pyelonephritis and cholecystitis in remission in the Truskavets’ spa. The examination was performed twice, before and after a 9-11-day course of balneotherapy. The subject of the study were the parameters of the electroencephalogram, heart rate variability, hormones and immunity. Results. The method of canonical correlation analysis states that the changes in immunity caused by balneotherapy are determined by the constellation of alaptation hormones by 98,9%, the autonomic nervous system by 99,9%, the central nervous system by 99,7%. Conclusion. Immunomodulatory effect of balneotherapy is realized through the neuro-endocrine mechanism

Relationships between the parameters of uric acid exchange and electroencephalograms in humans

Background. During the implementation of the project "Physiological activity of uric acid", our group discovered four variants of the combination of levels of uricemia and uricosuria both in healthy rats and in people with chronic pyelonephritis and cholecystitis in the remission phase, which are accompanied by characteristic constellations of parameters of the autonomic nervous, endocrine, and immune systems, as well as the exchange of nitrogenous metabolites and electrolytes, the levels of which correlate with uricemia and/or uricosuria. The aim of this study is to clarify the relationship between parameters of uric acid metabolism and electroencephalogram (EEG) in humans. Materials and methods. Under an observations were 34 males and 10 females by age 24-76 years with chronic pyelonephritis and cholecystitis in the phase of remission. The object of the study was serum and urine levels of uric acid (uricase method) as well as EEG. Results. It was identified 12 parameters as characteristic of uric acid metabolism clusters. Another 42 EEG parameters were found to be out of the discriminant model, despite the clear recognition ability. A strong canonical correlation between EEG parameters and uricemia (R=0,729) and a moderate correlation with uricosuria (R=0,553) were revealed. Even stronger connections were found between changes in parameters of uric acid metabolism and EEG under the influence of balneotherapy: R=0,901 and 0,681 respectively. Conclusion. Uric acid has both activating and inhibitory effects on EEG parameters. The neurotropic effect of uric acid as a structural analogue of methylxanthines and adenosine is realized, apparently, through various adenosine receptors

Role of autonomous and endocrine factors in immunotropic effects of nitrogenous metabolites in patients with chronic pyelonephritis

Background. We have previously shown that nitrogenous metabolites have immunomodulatory effects in healthy rats and humans as well as patients with dysfunction of neuroendocrine-immune complex, encephalopatia and chronic pyelonephritis. The purpose of this study is clarification of the role of neuro-endocrine factors in their immunotropic activity in patients with chronic pyelonephritis. Materials and Methods. The object of observation were 17 men (aged 24-70 years) with chronic pyelonephritis in remission. The plasma levels and urinary excretion of nitrogenous metabolites as well as parameters of immunity and its neuro-endocrine regulation twice (on admission and after balneotherapy at the Truskavets’ Spa) was performed. Results. Judging by the multiple correlation coefficient, uricosuria exhibits maximal neuro-endocrine activity (R=0,780), followed by bilirubinemia (R=0,742), creatinineuria (R=0,692), uricemia (R=0,636), creatinineemia (R=0,632), urea excretion (R=0,536), instead urea plasma correlate with neuro-endocrine parameters insignificantly (R=0,360). Nitrogenous metabolites together determine the state of neuro-endocrine regulation by 94,2%, which, in turn, determine the state of immunity by 99,9%. Conclusion. Nitrogenous metabolites carry out immunomodulation in different ways: directly through aryl hydrocarbon (bilirubin), toll-like and adenosine (uric acid) receptors of immunocytes; through modulation of the activity of neurons of the autonomous nervous system and endocrinocytes with subsequent neuro-endocrine immunomodulation; and also, apparently, due to an off-receptor effect on neurons, endocrinocytes and immunocytes (urea and creatinine)

Role of trace elements of mineral waters in their effects on neuro-endocrine-immune complex at female rats

Background. Drinking mineral water is one of the ethnopharmacologic factors. Previously, we identified the role of organic carbon and nitrogen of mineral waters in their effects on certain metabolic and neuro-endocrine-immune parameters. The purpose of this study is to clarify the role of trace elements of same mineral waters in their effects on neuro-endocrine-immune complex parameters. Material and methods. Experiment was performed on 48 healthy female Wistar rats 240-290 g divided into 5 groups. Animals of the first group for 6 days administered a single tap water through the tube at a dose of 1,5 mL/100 g of body mass. In the second group we administered the water Naftussya from the Truskavets’ layer (Galychyna, Ukraine), in the third group the water Sophiya of the Truskavets’ field. The rats of the fourth group received the native water from the Hertsa (Bucovyna, Ukraine) field, and the last group received its artificial salt analogue. The day after the completion of the drinking course in all rats HRV, endocrine and immunne parameters were registered. Results. The trace elements (fluoride, bromide, iodide, meta-silicic and orthoboric acids) of the chemical composition of loading fluids determine their effect on the neuro-endocrine parameters by 59%, on the immune parameters by 84%. Conclusion. The trace elements (fluoride, bromide, iodide, meta-silicic and orthoboric acids) of mineral waters play an essential role in their effects on the neuro-endocrine-immune parameters of female rats

Role of organic carbon and nitrogen of mineral waters in their immunomodulating effects at female rats

Background. Drinking mineral water is one of the ethnopharmacologic factors. Organic substances, despite their presence in all drinking mineral waters, are still considered to be active only in the water of Naftussya type (which, due to mineralization less than 1 g/L, are not formally mineral), whereas the physiological activity of true mineral waters (which mineralization is greater than 1-2 g/L) are associated with electrolytes and trace elements. Previously, we identified the role of organic carbon and nitrogen of mineral waters in their effects on certain metabolic and neuro-endocrine parameters. The purpose of this study is to clarify the role of organic substances of same mineral waters in their effects on immunity parameters. Material and methods. Experiment was performed on 48 healthy female Wistar rats 240-290 g divided into 5 groups. Animals of the first group for 6 days administered a single tap water through the tube at a dose of 1,5 mL/100 g of body mass. In the second group we administered the water Naftussya from the Truskavets’ layer (Galychyna, Ukraine), in the third group the water Sophiya of the Truskavets’ field. The rats of the fourth group received the native water from the Hertsa (Bucovyna, Ukraine) field, and the last group received its artificial salt analogue. The day after the completion of the drinking course in all rats immunne parameters were registered. Results. On the basis of the correlation analysis with step-by-step exclusion, 9 immune parameters (Endotheliocytes of Thymus, Spleen Mass Index, Entropy of Splenocytogram, Natural Killers, B- and 0-Lymphocytes, Rod-shaped Neutrophils, Phagocytic Index and Microbian Count of Monocytes of Blood) are included in the regressive model for organic nitrogen (R=0,818) and 6 parameters (Endotheliocytes of Thymus, Fibroblastes and Reticulocytes of Spleen, Pan-Lymphocytes, Microbian Count of Neutrophils and Phagocytic Index of Monocytes of Blood) for organic carbon (R=0,690). Together, the organic components of the chemical composition of loading fluids determine their effect on the immune parameters by 77%. Conclusion. Organic substances of mineral waters play an essential role in their effects on the immune parameters of female rats

Relationships between changes in EEG and some metabolic parameters in patients of Truskavets’ spa

Background. Earlier, we found relationships between EEG/HRV parameters and plasma/ urine electrolytes concentration in Truskavets’ spa patients. The aim of this study is to analyze the relationships between changes in these parameters under the influence of balneotherapy. Materials and Methods. The object of observation were 22 men (23-70 years), who underwent rehabilitation treatment of chronic pyelonephritis and cholecystitis in remission. The examination was performed twice, before and after a 9-11-day course of balneotherapy. The object of the study were the parameters of the EEG and exchange of electrolytes and nitric metabolites. Results. Judging by the coefficient of determination, changes in the concentrations of electrolytes and nitric metabolites in the urine and plasma are mediated by changes in neurodynamics by 99,8 % and 99,4% respectively. Conclusion. Balneotherapy affects the exchange of electrolytes and nitrogen metabolites through the CNS. &nbsp

Uric acid, neuroendocrine-immune complex and metabolism: relationships

Background. During the implementation of the project "Physiological activity of uric acid", our group discovered four variants of the combination of levels of uricemia and uricosuria in patients with chronic pyelonephritis in the remission phase, which are accompanied by characteristic constellations of parameters of the central and autonomic nervous, endocrine, and immune systems, as well as the exchange of nitrogenous metabolites and electrolytes, the levels of which correlate with uricemia and/or uricosuria. The aim of this study is to clarify the relationship between parameters of uric acid exchange and neuroendocrine-immune complex as well as other metabolites. Materials and methods. Under an observations were 34 males (23-70 years) and 10 females (33-76 years) with chronic pyelonephritis in the phase of remission. The main object of the study was serum and urine levels of uric acid. Other metabolic, endocrine and immune parameters were determined in the same blood and urine samples. In addition, EEG and HRV was recorded almost synchronously. Results. Among all registered parameters, 28 were identified as characteristic of the four variants of uric acid metabolism. The discriminant model includes, in addition to uricosuria and uricemia by definition, 10 neuroendocrine, 5 immune, and 6 metabolic parameters, as well as bacteriuria, Bifidobacteria of feces, entropy of immunocytogram and Popovych’s leukocytogram strain index. According to the results of the canonical correlation analysis, it was established that balneotherapy-induced concomitant changes in uricosuria and uricemia positively determine changes in, first, the PSD of the theta-rhythm in the T3 locus and beta-rhythm in the O2 locus aa well as HRV-markers of vagal tone and sympatho-vagal balance; secondly – diuresis and excretion of urea, magnesium, sodium, phosphates, calcium, potassium and chloride, as well as calciumemia and magnesiumemia; thirdly – serum IgG and CIC. Instead, changes in cortisolemia and testosteroneemia as well as TNF-alpha and the intensity of Staph. aureus phagocytosis are subject to negative determination. In general, the rate of uric acid determination of the dynamics of the listed parameters of the body is 96%. Conclusion. The uric acid molecule, as a structural analog of methylxanthines and adenosine, exerts effects on neurons, endocrinocytes, and immunocytes, presumably through their adenosine receptors, and the metabolic effects of uric acid are the consequences of its neuro-endocrine effects

ОЦІНКА ЕФЕКТИВНОСТІ ВПРОВАДЖЕННЯ ПРОГРАМИ ОПТИМІЗАЦІЇ РОЗВИТКУ ТА ПСИХОКОРЕКЦІЇ СОЦІАЛЬНИХ ОЧІКУВАНЬ ОСОБИСТОСТІ

У статті обґрунтовано змістово-процесуальні особливості програми оптимізації розвитку та психокорекції соціальних очікувань особистості. Визначено принципи, описано організацію і умови проведення формувального етапу дослідження. Подано програму тренінгових занять та післятренінгового супроводження «Очікуваний результат». Розкрито сутність напрямків формувально-корекційного етапу програми дослідження: формувально-розвивального, профілактики та психокорекції. Окреслено доцільність і змістові особливості післятренінгового супроводження. Проведено контрольний етап експериментального дослідження. Розглянуто головні результати впровадження програми дослідження соціальних очікувань особистості. Оцінено ефективність впровадження програми оптимізації розвитку та психокорекції соціальних очікувань особистості. Здійснено обґрунтування отриманих експериментальних результатів дослідження

Peculiarities of modulating effects of “Myroslava” and “Khrystyna” mineral waters on neuroendocrine-immune complex and metabolism in patients of Truskavets’ spa

Background. Earlier we found effects on the neuroendocrine-immune complex and metabolism, which are common to the mineral waters "Myroslava" and "Khrystyna", instead different from the effects of Naftussya water. The purpose of this article is to find the specific effects of these waters. Materials and Methods. The object of clinical-physiological observation were 22 men aged 23-70 years, who underwent rehabilitation treatment of chronic cholecystitis and pyelonephritis in remission in the Truskavets’ spa. The examination was performed twice, before and after a 7-10-day course of balneotherapy. Half of the patients received bioactive water Naftussya and sulfate-chloride sodium-magnesium mineral water "Myroslava" (M 5 g/L), and the other half - Naftussya and "Khrystyna" (M 10 g/L). The subject of the study were the parameters of the neuroendocrine-immune complex, microbiota, cholekinetics and metabolism. Results. Screening revealed specific changes in 37 parameters grouped into 5 patterns. The first pattern combines 11 parameters that decrease under the influence of "Myroslava" water (Myr), while increase under the influence of "Khrystyna" water (Khr). For the other 7 parameters of the second pattern, Myr acts similarly, while Khr is ineffective. Both mineral waters have a stimulating effect on 8 parameters, while Myr is inferior to Khr. Myr has a upregulating effect on 9 parameters of the fourth pattern, while Khr has a downregulating effect. Finally, sodium excretion and leukocyturia are reduced under the influence of both waters, but to a greater extent under the influence of Khr. Conclusion. The newly created sulfate-chloride sodium-magnesium drinking mineral waters of Truskavets’ spa have both common and specific favorable modulating neuroendocrine, metabolic and immune effects on patients with chronic cholecystitis and pyelonephritis