Hitchhiking Through the Cytoplasm

We propose an alternative mechanism for intracellular cargo transport which results from motor induced longitudinal fluctuations of cytoskeletal microtubules (MT). The longitudinal fluctuations combined with transient cargo binding to the MTs lead to long range transport even for cargos and vesicles having no molecular motors on them. The proposed transport mechanism, which we call ``hitchhiking'', provides a consistent explanation for the broadly observed yet still mysterious phenomenon of bidirectional transport along MTs. We show that cells exploiting the hitchhiking mechanism can effectively up- and down-regulate the transport of different vesicles by tuning their binding kinetics to characteristic MT oscillation frequencies

A Solvable Model for Polymorphic Dynamics of Biofilaments

We investigate an analytically tractable toy model for thermally induced polymorphic dynamics of cooperatively rearranging biofilaments - like microtubules. The proposed 4 -block model, which can be seen as a coarse-grained approximation of the full polymorphic tube model, permits a complete analytical treatment of all thermodynamic properties including correlation functions and angular fourier mode distributions. Due to its mathematical tractability the model straightforwardly leads to some physical insights in recently discussed phenomena like the "length dependent persistence length". We show that a polymorphic filament can disguise itself as a classical worm like chain on small and on large scales and yet display distinct anomalous tell-tale features indicating an inner switching dynamics on intermediate length scales

Why Microtubules run in Circles - Mechanical Hysteresis of the Tubulin Lattice

The fate of every eukaryotic cell subtly relies on the exceptional mechanical properties of microtubules. Despite significant efforts, understanding their unusual mechanics remains elusive. One persistent, unresolved mystery is the formation of long-lived arcs and rings, e.g. in kinesin-driven gliding assays. To elucidate their physical origin we develop a model of the inner workings of the microtubule's lattice, based on recent experimental evidence for a conformational switch of the tubulin dimer. We show that the microtubule lattice itself coexists in discrete polymorphic states. Curved states can be induced via a mechanical hysteresis involving torques and forces typical of few molecular motors acting in unison. This lattice switch renders microtubules not only virtually unbreakable under typical cellular forces, but moreover provides them with a tunable response integrating mechanical and chemical stimuli.Comment: 5 pages, 4 Movies in the Supplemen

High-T_{c} Superconductors with AF Order: Limitations on Spin-Fluctuation Pairing Mechanism

The very intriguing antagonistic interplay of antiferromagnetism (AF) and superconductivity (SC), recently discovered in high-temperature superconductors, is studied in the framework of a microscopic theory. We explain the surprisingly large increase of the magnetic Bragg peak intensity $I_{Q}$ at $Q\sim (\pi ,\pi)$ in the magnetic field $H\ll H_{c2}$ at low temperatures $0<T\ll T_{c},T_{AF}$ in $La_{2-x}Sr_{x}CuO_{4}$. Good agreement with experimental results is found. The theory predicts large anisotropy of the relative intensity $R_{Q}(H)=(I_{Q}(H)-I_{Q}(0))/I_{Q}(0)$%, i.e. $R_{Q}(H\parallel c-axis)\gg R_{Q}(H\perp c-axis)$. The quantum (T=0) phase diagram at H=0 is constructed. The theory also predicts: (i) the magnetic field induced AF order in the SC state; (ii) small value for the spin-fluctuation coupling constant $g<(0.025-0.05)$ $eV$. The latter gives very small SC critical temperature $T_{c}(\ll 40$ $K)$, thus questioning the spin-fluctuation mechanism of pairing in HTS oxides.Comment: Linguistic changes, improved readabilty, changed titl

Crunching Biofilament Rings

We discuss a curious example for the collective mechanical behavior of coupled non-linear monomer units entrapped in a circular filament. Within a simple model we elucidate how multistability of monomer units and exponentially large degeneracy of the filament's ground state emerge as a collective feature of the closed filament. Surprisingly, increasing the monomer frustration, i.e., the bending prestrain within the circular filament, leads to a conformational softening of the system. The phenomenon, that we term polymorphic crunching, is discussed and applied to a possible scenario for membrane tube deformation by switchable dynamin or FtsZ filaments. We find an important role of cooperative inter-unit interaction for efficient ring induced membrane fission

Reshaping and Enzymatic Activity allow Viruses to move through the Mucus

Filamentous viruses like influenza and torovirus often display systematic bends and arcs of mysterious physical origin. We propose that such viruses undergo an instability from a cylindrically symmetric to a toroidally curved state. This "toro-elastic" state emerges via a spontaneous symmetry breaking under prestress, induced via short range spike protein interactions and magnified by the filament's surface topography. Once surface stresses become sufficiently large, the filament buckles and the toroidal, curved state constitutes a soft mode that can propagate through the filament's material frame around a "mexican-hat" potential. In the mucus of our airways, glycan chains are omnipresent that influenza's spike proteins can bind to and cut. We show that when coupled to such a non-equilibrium chemical reaction, the curved toro-elastic state can attain a spontaneous rotation for sufficiently strong enzymatic activity, leading to a whole body reshaping propulsion similar to -- but different from -- eukaryotic flagella and spirochetes.Comment: 6 pages, Supplementary Info (PDF file in the source file

Equation of State of Looped DNA

We derive the equation of state of DNA under tension that features a loop. Such loops occur transiently during DNA condensation in the presence of multivalent ions or permanently through sliding protein linkers such as condensin. The force-extension relation of such looped-DNA modeled as a wormlike chain is calculated via path integration in the semiclassical limit. This allows us to rigorously determine the high stretching asymptotics. Notably the functional form of the force-extension curve resembles that of straight DNA, yet with a strongly renormalized apparent persistence length. We also present analogous results for DNA under tension with several protein-induced kinks and/or loops. That means that the experimentally extracted single-molecule elasticity does not necessarily only reflect the bare DNA stiffness, but can also contain additional contributions that depend on the overall chain conformation and length

Helices at Interfaces

Helically coiled filaments are a frequent motif in nature. In situations commonly encountered in experiments coiled helices are squeezed flat onto two dimensional surfaces. Under such 2-D confinement helices form "squeelices" - peculiar squeezed conformations often resembling looped waves, spirals or circles. Using theory and Monte-Carlo simulations we illuminate here the mechanics and the unusual statistical mechanics of confined helices and show that their fluctuations can be understood in terms of moving and interacting discrete particle-like entities - the "twist-kinks". We show that confined filaments can thermally switch between discrete topological twist quantized states, with some of the states exhibiting dramatically enhanced circularization probability while others displaying surprising hyperflexibility

Rolling and ordering of micro rods in shear flow induced by rod wall interactions

Dynamics of micro particles close to interfaces is a relevant topic in Soft Matter. Translational and rotational dynamics of particles possessing different shapes govern a broad range of interfacial phenomena from biofilm formation, drug delivery and particle active rolling motion. These dynamics usually occur in the presence of external fields such as shear flows, electric fields and gravity. By experiments and theoretical models, we investigate the rolling and translational motion of rod-shaped micro particles close to a solid wall in the presence or absence of a shear flow. Hydrodynamics, long-range surface forces and Brownian motion act on the micro rods, which show non-trivial dynamics such as translational motion orthogonal to the flow direction and preferential ordering with the rod long axis perpendicular to the flow direction