Media 4: PSF shaping using adaptive optics for three-dimensional single-molecule super-resolution imaging and tracking

Originally published in Optics Express on 27 February 2012 (oe-20-5-4957

Media 2: PSF shaping using adaptive optics for three-dimensional single-molecule super-resolution imaging and tracking

Originally published in Optics Express on 27 February 2012 (oe-20-5-4957

Media 1: PSF shaping using adaptive optics for three-dimensional single-molecule super-resolution imaging and tracking

Originally published in Optics Express on 27 February 2012 (oe-20-5-4957

One-Shot Measurement of the Three-Dimensional Electromagnetic Field Scattered by a Subwavelength Aperture Tip Coupled to the Environment

Near-field scanning optical microscopy (NSOM) achieves subwavelength resolution by bringing a nanosized probe close to the surface of the sample. This extends the spectrum of spatial frequencies that can be detected with respect to a diffraction limited microscope. The interaction of the probe with the sample is expected to affect its radiation to the far field in a way that is often hard to predict. Here we address this question by proposing a general method based on full-field off-axis digital holography microscopy which enables to study in detail the far-field radiation from a NSOM probe as a function of its environment. A first application is demonstrated by performing a three-dimensional (3D) tomographic reconstruction of light scattered from the subwavelength aperture tip of a NSOM, in free space or coupled to transparent and plasmonic media. A single holographic image recorded in one shot in the far field contains information on both the amplitude and the phase of the scattered light. This is sufficient to reverse numerically the propagation of the electromagnetic field all the way to the aperture tip. Finite Difference Time Domain (FDTD) simulations are performed to compare the experimental results with a superposition of magnetic and electric dipole radiation