2 research outputs found
Virulence profile of tigecycline-resistant Gram-negative bacilli isolated from river waters using the Caenorhabditis elegans infection model / Perfil de virulência de bacilos Gram-negativos resistentes à tigeciclina isolados das águas dos rios usando o modelo de infecção por Caenorhabditis elegans
Last-resort antibiotics act as ultimate force to overcome multidrug-resistant strains infections. Cases of tigecycline resistance in gram-negative bacilli in clinical settings are reported worldwide, however, there is no data related to tigecycline resistant strains in river water. This study demonstrates seven tigecycline gram-negative bacilli isolated from river water in Rio de Janeiro metropolitan area, their resistance genes, ability of biofilm formation with/without antibiotics and behavior using the nematode Caenohabidits elegans as infection in vivo model. From 24 gram-negative isolated strains, 16 (66.6%) were classified as multidrug-resistant, however, seven (29.1%) presented resistant to all antimicrobial agents tested, including tigecycline and have been identified by MALDI-TOF as A. baumannii, E. aerogenes and P. agglomerans. All tigecycline-resistant strains presented amplification products for ESBL, AME and PMQR and ability of biofilm formation on hydrophilic and hydrophobic abiotic surfaces with and without antimicrobial agents. The presence of antimicrobials did not inhibit biofilm formation. Tigecycline-resistant strains differed of OP50 control with P<0,0001 indicating its virulence potential, however, none of them were capable to kill all nematodes during 5 days infection. In conclusion, tigecycline-resistant gram-negative strains have important global public health implications due to the therapeutic problems they pose. Further studies and continuous surveillance of tigecycline-resistant strains in both clinical and aquatic environment remains necessary to track and understand the dissemination of tigecycline resistance