9 research outputs found
LHC Benchmarks from Flavored Gauge Mediation
We present benchmark points for LHC searches from flavored gauge mediation
models, in which messenger-matter couplings give flavor-dependent squark
masses. Our examples include spectra in which a single squark - stop, scharm,
or sup - is much lighter than all other colored superpartners, motivating
improved quark flavor tagging at the LHC. Many examples feature flavor mixing;
in particular, large stop-scharm mixing is possible. The correct Higgs mass is
obtained in some examples by virtue of the large stop A-term. We also revisit
the general flavor and CP structure of the models. Even though the A-terms can
be substantial, their contributions to EDM's are very suppressed, because of
the particular dependence of the A-terms on the messenger coupling. This holds
regardless of the messenger-coupling texture. More generally, the special
structure of the soft terms often leads to stronger suppression of flavor- and
CP-violating processes, compared to naive estimates.Comment: 32 pages, 11 figures. Updated to published versio
Centering and symmetry breaking in confined contracting actomyosin networks
Centering and decentering of cellular components is essential for internal organization of cells and their ability to perform basic cellular functions such as division and motility. How cells achieve proper localization of their organelles is still not well-understood, especially in large cells such as oocytes. Here, we study actin-based positioning mechanisms in artificial cells with persistently contracting actomyosin networks, generated by encapsulating cytoplasmic Xenopus egg extracts into cell-sized ‘water-in-oil’ droplets. We observe size-dependent localization of the contraction center, with a symmetric configuration in larger cells and a polar one in smaller cells. Centering is achieved via a hydrodynamic mechanism based on Darcy friction between the contracting network and the surrounding cytoplasm. During symmetry breaking, transient attachments to the cell boundary drive the contraction center to a polar location. The centering mechanism is cell-cycle dependent and weakens considerably during interphase. Our findings demonstrate a robust, yet tunable, mechanism for subcellular localization
Scaling behaviour in steady-state contracting actomyosin networks
Contractile actomyosin network flows are crucial for many cellular processes including cell division and motility, morphogenesis and transport. How local remodelling of actin architecture tunes stress production and dissipation and regulates large-scale network flows remains poorly understood. Here, we generate contracting actomyosin networks with rapid turnover in vitro, by encapsulating cytoplasmic Xenopus egg extracts into cell-sized ‘water-in-oil’ droplets. Within minutes, the networks reach a dynamic steady-state with continuous inward flow. The networks exhibit homogeneous, density-independent contraction for a wide range of physiological conditions, implying that the myosin-generated stress driving contraction and the effective network viscosity have similar density dependence. We further find that the contraction rate is roughly proportional to the network turnover rate, but this relation breaks down in the presence of excessive crosslinking or branching. Our findings suggest that cells use diverse biochemical mechanisms to generate robust, yet tunable, actin flows by regulating two parameters: turnover rate and network geometry