A study of surface water pollution with azithromycin in Ukraine

Introduction: Water pollution with antibiotics plays a key role in the formation and spread of antibiotic resistance, which threatens humanity and the environment as a whole.Aim: The purpose of our work was to develop a method for determining azithromycin in wastewater and surface water using the thin-layer chromatography method.Materials and Methods: The developed technique was tested in the wastewater of Zolochiv district of Kharkiv region.Results and Discussion: Using conventional analytical scales and universal chromatography in thin layers of a sorbent, it is possible to identify azithromycin with a water concentration of ≥ 30 µg/mL without complex and expensive equipment, such as HPLC or LC/MS/MS. The results showed that the concentration of azithromycin is less than 30 µg/mL

The synthesis and the antioxidant activity of 1-phenoxymethyl-4-aryl-5,6,7,8-tetrahydro-2a,4a,8a-triazacyclopenta[cd]azulene-3-carboxylic (or carbothionic) acid derivatives

New 1-phenoxymethyl-4-aryl-5,6,7,8-tetrahydro-2а,4a,8a-triazacyclopenta[cd]azulene-3-carboxylic (or carbothionic) acid derivatives have been designed, synthesized and evaluated for their in vitro antioxidant activity under conditions of the artificial oxidative stress using ionol, ascorbic acid and α-tocopherol as the reference drugs. It has been found that 1-phenoxymethyl-4-aryl-5,6,7,8-tetrahydro-2а,4a,8a-triazacyclopenta[cd]azulene-3-carbothionic acid derivatives 9b, 9c, 9d, 9e, 9f, 9i and 1-phenoxymethyl-4-(41-chlorophenyl)-5,6,7,8-tetrahydro-2,2a,8-triazacyclopenta[cd]azulene-3-carboxylic acid phenylamide 10 reveal a high antioxidant activity and a good in silico pharmacokinetic profile. The data obtained allowed us to select the most promising objects from the substances synthesized for further pharmacological screening for the presence of the antioxidant activity in vivo