6 research outputs found
Schematic of the calculation of transformed fluorescence ratio <i>k'</i>.
<p>Schematic of the calculation of transformed fluorescence ratio <i>k'</i>.</p
The transformed fluorescence ratio <i>kβ</i> comprising 4 runs of plasmid mix (run1-4) and 3 runs of pooled aphids (run5β7) with predefined RAF.
<p>The transformed fluorescence ratio k' is the transformation of the ratio of two fluorescence intensities when one fluorescence reaches its inflexion point.</p><p><b>RAF</b>: Predefined Resistance allele frequency (RAF) expressed as percentage.</p><p><i>Kβ</i>: Average of transformed fluorescence ratio (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0091104#pone.0091104.e017" target="_blank">equation 14</a>) from 3 replicates.</p><p>Inter run CV (%): the average coefficient of variation for a standard among 7 runs.</p><p>Intra run CV (%): the average coefficient of variation for 13 standards within a run.</p><p>*: No replicate.</p
Prediction of RAF by normalizing kβ to Run1 T/S.
<p>RAF*: Predicted resistance allele frequency from Run1 T/S.</p><p><i>k'</i> adj**: normalized transformed fluorescence ratio kβ based on Run1 T/S.</p
Field isolates of <i>Aphis gossypii</i> collected during the 2011/2012 season showing pirimicarb resistance status determined by individual PCR-RFLP.
<p>R: Resistant to Pirimicarb.</p><p>S: Susceptible to Pirimicar.</p
Raw fluorescence plot of TaqMan assay with two probes.
<p>Fam probe (blue) was from resistance allele and Quasar probe (red) was from susceptible allele.</p
Coefficient of determination R<sup>2</sup> of inter-run <i>kβ</i> with linear and 4 parameter sigmoid curve fitting.
<p>Run1β4 are plasmid mix and run5β7 are pooled aphids.</p