PERAN OVISIDAL HERBAL SERBUK BIJI PEPAYA MATANG DAN ALBENDAZOL TERHADAP DAYA BEREMBRIO TELUR CACING Ascaris suum SECARA IN VIVO

Telah dilakukan penelitian untuk mengetahui pemberian herbal serbuk biji pepaya matang secara oral pada babi ascariasis terhadap daya berembrio telur cacing pada tinja dan pada uterus cacing yang keluar dari babi yang diobati tersebut. Pada percobaan ini sebanyak 24 ekor babi Landrace betina dengan bobot badan ± 10-15 kg, umur ± 15 minggu yang terinfeksi cacing Ascaris suum (A. suum) secara alami, dengan egg per gram (EPG) berkisar 250-2500 butir. Babi tersebut dibagi menjadi empat kelompok perlakuan (P0, P1, P2, dan P3) masing-masing terdiri dari 6 ekor. Kelompok P1 dan kelompok P2 mendapat perlakuan dengan herbal serbuk biji pepaya matang per oral dengan dosis masing-masing 1 dan 3 g/kg bobot badan (P2) selama 3 hari berturut-turut. Kelompok P3 mendapat perlakuan dengan albendazol dosis 0,5 mg/kg bobot badan (Zodalben 0,04 ml/kg bobot badan) sedangkan kelompok P0 bertindak sebagai kontrol tanpa pengobatan. Hasil penelitian ini menunjukkan bahwa pengobatan dengan herbal serbuk biji pepaya matang pada babi ascariasis ternyata mampu menurunkan daya berembrio telur cacing dalam tinja babi dan dalam uterus cacing secara efektif sehingga dapat digunakan untuk pengendalian askariasis pada babi

Suplementasi Mineral Pada Pakan Sapi Bali Terhadap Diferensial Leukosit Di Empat Tipe Lahan (MINERAL SUPPLEMENTATION ON BALINESE CATTLE TOWARD LEUCOCYTE DIFFERENTIAL IN FOUR TYPES OF LAND)

The aims of this research were to know the effect of mineral intake on Balinese cattle that has been fed on four different types of land (rice field, grassland, garden, and forest) towards theleukocytes differential.  The research design follows a completely randomized design (CRD) usinga nested pattern on 48 males from Balinese cattle.  The cattle were grouped according to four treatments of mineral intake, namely: controls, 2.5 grams, five grams, and 7.5 grams in each type ofland.  Furthermore, the blood was taken from the auricularis superficialis vein in the third month after treatment. A blood smear was prepared using the slide method and a sample was stained withGiemsa. The blood smear was examined by leukocytes differential using battlement method with 1000x magnification. Then, the collected data were analyzed using analysis of variance and Cochran test.  The research results show that the given treatment of minerals has no change theleukocytes differential.  In contrast, the percentage of lymphocytes in the type of garden land haslower than on the type of forest, rice land and grassland, but has no differences the percentage of neutrophils, eosinophils, basophils, and monocytes in the four types of land. The conclusion isgiving to a concentration of 7.5 gram does not change the differential leukocyte Bali cattle

THE OVICIDAL EFFECT OF ALBENDAZOLE AGAINST WORM EGGS OF PARAMPHISTOMUM SPP. BY IN-VITRO

Albendazole is one of the modern anthelmintics that has effect vermicidal, larvicidal, and ovicidal. The sample used is the eggs of worms Paramphistomum spp., obtained from the rumen of Bali cattle. This research is an experimental research laboratory and using complete random design. This study uses four different treatments with five repetitions so retrieved 20 types of worm eggs of Paramphistomum spp. research methods include the beginning of a collection of egg worm of Paramphistomum spp., the dose given that is 0,06 mL Albendazole/40mL NaCl (P1), 0,12mL Albendazole /40mL NaCl (P2), 0,24 mL Albendazole/40 mL NaCl (P3) and control without treatment (P0). The eggs were observed on the 10th day and the 30th day, and then counting the number of eggs that do not hatch. The data obtained were tested statistically with ANOVA test and proceed with Duncan test to see the differences between the treatments. Albendazole has ovicidal effect against the worm eggs of Paramphistomum spp. by invitro. The doses of 0,24mL Albendazole/40mL NaCl (P3) give the highest percentage number worm eggs of Paramphistomum spp. are not hatching with 16.85%.

ALBUMIN LEVELS OF BALI CATTLE THAT INFECTED BY FASCIOLA GIGANTICA

Albumin is a plasma protein that is most widely in the body and serves to maintain the osmotic pressure of blood. Albumin levels on Bali cattle are infected by various factors, one of which is a worm infection Fasciola gigantica. The purpose of this research is to determine the levels of albumin Bali cattle that infected by Fasciola gigantica. This research is using 60 blood samples of Bali cattle, consisting of 30 blood samples Bali cattle that infected by Fasciola gigantica and 30 blood samples of healthy Bali cattle. This research are using cross-sectional studies methods, samples were taken at the Slaughter House Pesanggaran, Denpasar. Analysis of the data is using the onesample t test, comparing samples that infected with the healthy samples. The results showed that the average levels of albumin Bali cattle infected with Fasciola gigantica was 3.24 g/dL and the average albumin levels were not infected with Fasciola gigantica was 4.14 g/dL. The data analysis showed that the infection of Fasciola gigantica highly significant (P<0.01) towards decreased levels of albumin Bali cattle at 0.9 g/dL. It is concluded that infected Bali cows that are slaughtered in the Slaughterhouse Pesanggaran suffered liver disfunction

OVICIDAL EFFECT WUDANI LEAF EXTRACT AGAINST EGGS FASCIOLA GIGANTICA WORM BY IN-VITRO

Bali cattle is a source of meat that has high economic value. One endoparasite disease that attacks cattle and very detrimental to farmers is Fascioliasis caused by Fasciola gigantica worm. This study aims to determine the ovicidal effect of the extract of wudani leaf against Fasciola gigantica worm eggs in-vitro and determine the effective dose of it. This study used a completely randomized design (CRD) consist of 4 treatments, each treatment consisted of 5 replicates so that the number of samples are 20 observations. Obtained data were tested with Analyze of Variant followed by Duncan's Multiple Range Test. The results of the study at day 10 indicated the ovicidal effect wudani leaf extract to doses 0,24mL / 40 NaCl physiological and dose 0,48mL /40 physiological NaCl was higher than the dose 0,12mL / 40 Physiological NaCl (P 0.01), while the results at day 30 showed a significant result that the dose 0,48mL / 40mL physiological NaCl was higher than the dose 0,24mL / 40mL physiological NaCl, the dose 0,12mL / 40mL physiological NaCl and control (P <0.01)

Prosentase Kejadian Infiltrasi Limfoblas Di Hati, Limpa Dan Ginjal- Ayam Strain Super Hargo-Hy-Line Dan Decale Yang Menderita Limfoid Leukosis

Pada hnti d:tn limpa ayam petelur yang menderita 1 i mfoid Leukosi s ( 11 ) , terinfiltrasi secara hebat o1eh sel 1imfobL. s , secL ngkan ginja1 1 ebih ringan jika dibandingkan dengan ke dua organ t e r sebut . !Jn di1 akukan pengarnat an seba nyal-\2 48 b1ok j aringan h3ti , l impR dan ginja1 ay am pete1ur yang menderita 11, s ec or a histopato1o 0 ik d en ~; an r:1 empergunakan pewarnaan Henwtoxylin dan Eosin ( H danE), t crhadap p rosent a sc k ejadian infi1- trr.si 1infob1as . Ibsi1 y an g dip.ero}eh ada1ah 100 % -pada hA.ti dan 1i mpa DYotrl y0ng di amati terinfi1 trasi o1eh se1 1imfob1a s . Sed.:mg - kan kej ad;Lon pada ginj a l 81,25 %, n amun pn.da Uji x2 pcrbcdaan t erse but tidal<:: nyata pada p ) 0 , 05. Bi1 a ditinj<i.U dari str~ i n ayam , yang t erdiri dnri 23 ekor -straip. Super Harco , 15 ekor Hy- 1ine dan 1q ekor Deca1b, n au pun da_i u .mr ayam y a ng dapat terserang, Yc=li tu 14 sampA.i , 1 ebih kecj_1 16 minggu ( 7 ekor ) , 16 sax:tpai 2L~ minggu ( 2Lr - ekor ) dan di atas 24 minggu ( 17 ekor ), t e r nyGta kejadian infi1trasi limfob1 as di hati dan 1impa tidak berbeda ( 100 ~) . Sedangkan pada ginjal ke tiga str-t;in. ayam t e r sebut t adal ah b crb c da y0itu 91,3 % ( Super Harco ), 73 ,3% ( Hy - line ) dan 29 . 70 ,0 % ( DecR1b ), Dan ke jadian infi1trasi 1imfob1as pada ginja1 dari masing mEtsing umur ayam yane diamati berturut ·_ turut a da 1ah 71,4 ~ ( 14 s am~o i l ebih keci1 16 minggu ) , 87 , 5 % ( 16 sampai 24 rrli:nggu.) dan 82,4 % ( di a t as 24 minggu ) • J1k.:1n tetapi pada Uji J!. per bedaan tersebut tidak nya t ,~ pada p ) 0,05

Penurunan Angka Morbiditas dan Mortalitas Anak Babi yang Diberi Vitamin dan Elektrolit Melalui Air Minum Saat Disapih

The present work was aimed to study the effect of oral treatment of piglets with vitaminand electrolyte at the time of weaning on their morbidity and mortality rates. A number of160 piglets at 28 – 35 days of age were randomly allotted into 4 groups. The control group(P0) received only drinking water whereas the treatment groups were supplied with vitaminand electrolyte (Superfite Forte) for 6 hours a day and for 3 consecutive days at variousdosages; treatment group 1 (P1) received 1 gram/l water, P2: 2 gram/l water and P3: 3gram/l water. Apart from that, drinking water was given ad libitum. Observation andrecording of illness and death was carried out every day from the day of weaning till 21days later. The results showed that treatment with vitamin and electrolyte at 1 – 3 gram/lwater significantly reduced the morbidity and mortality rates. Thus, it is recommended thatpiglets at the time of weaning should be supplied with vitamin and electrolyte in order toreduce the morbidity and mortality rates

STRATEGI PENCEGAHAN PENYAKIT INEFEKSIUS PADA PETERNAKAN BROILER BERBASIS LABORATORIUM

Infectious diseases in broilers remain a problem caused huge economic losses in the form;performance (high mortality and mobidity, slow growth rate, bad feed conversion ratio(FCR), and low meat production), increaase medical costs, should performdecontamination (cleaning and disinfection) and revaccination. Failure on due to diseasethat occurs in broilers caused by the breeder has not completely make handling the diseaseis due to lack of management for prevention the disease. This paper proposed a strategy fordisease prevention by promoting bio-security measures, vaccination and medication-basedlaboratory.beternak aya

Respon Imun Anak Babi Pasca Vaksinasi Hog Cholera

Penelitian ini bertujuan untuk mengetahui pengaruh antibodi maternal terhadap titer antibodi anak babi yang di vaksin hog cholera umur 7 hari. Penelitian menggunakan tujuh sampel babi dari induk yang divaksin secara teratur yang diberikan perlakuan vaksinasi pada umur 7 hari. Pengambilan sampel serum dilakukan pravaksinasi (7 hari), dan satu minggu serta dua minggu pasca vaksinasi. Untuk menentukan titer antibodi virus Hog cholera pada sampel anak babi dilakukan uji ELISA. Data yang diperoleh kemudian dianalisis mengunakan paired sampel T test antara titer antibodi hog cholera. Hasil paired sample T test menunjukkan terjadinya penurunan titer antibodi maternal yang nyata (p<0,05) pada pra vaksinasi ( umur 7 hari ) dengan satu minggu pasca vaksinasi dan sangat nyata (p<0,01) dengan hari dua minggu pasca vaksinasi. Dari hasil penelitian ini dapat disimpulkan bahwa antibodi maternal yang tinggi akan mengakibatkan penurunan pada titer antibodi pasca vaksinasi. Perlu dilakukan penelitian lebih lanjut untuk mengetahui waktu vaksinasi yang efekti

Kombinasi Tylosin dan Enrofloxacin dalam Ransum terhadap Total Leukosit dan Diferensial Leukosit Babi Landrace Pascasapih

Penelitian ini bertujuan untuk mengetahui pengaruh kombinasi tylosin dan enrofloxacin pada ransum terhadap total dan diferensial leukosit babi Landrace pascasapih umur 35-42 hari. Sampel yang digunakan adalah darah dari 24 ekor babi Landrace pascasapih. Perlakuan yang diberikan terdiri dari 4 kelompok, yakni babi yang diberi ransum tanpa kombinasi tylosin dan enrofloxacin (P0) dan babi yang mendapat ransum dengan kombinasi tylosin dan enrofloxacin masing-masing 1000 ppm (P1), 2000 ppm (P2), dan 4000 ppm (P3). Pemberian perlakuan diberikan selama satu kali dalam sehari selama 7 hari. Hasil penelitian yang didapat berurutan dari P0, P1, P2, P3, jumlah rerata total leukosit (103/µl) sebesar (25,40; 26,54; 28,47; 29,40), rerata diferensial leukosit (103/µl) neutrofil (8,04; 9,11; 10,76; 12,65), eosinofil (1,02; 1,55; 1,88; 2,09), basofil (0,19; 0,28; 0,35; 0,39), limfosit (9,68; 10,68; 12,34; 13,59) dan monosit (1,02; 1,24; 2,00; 2,05). Hasil tersebut menunjukkan bahwa kombinasi tylosin dan enrofloxacin pada ransum meningkatkan total dan diferensial leukosit babi Landrace pascasapih, namun peningkatan yang ditunjukkan tidak melebihi dari rentang nilai normal