Radiochemical synthesis of105gAg-labelled silver nanoparticles

A method for synthesis of radiolabelled silver nanoparticles is reported. The method is based on proton activation of silver metal powder, enriched in 107Ag, with a 30.7 MeV proton beam. At this proton energy 105gAg is efficiently created, mainly via the 107Ag(p,3n)105Cd → 105gAg reaction. 105gAg has a half-life of 41.29 days and emits easily detectable gamma radiation on decay to 105Pd. This makes it very useful as a tracing radionuclide for experiments over several weeks or months. Following activation and a period to allow short-lived radionuclides to decay, the powder was dissolved in concentrated nitric acid in order to form silver nitrate (AgNO3), which was used to synthesise radiolabelled silver nanoparticles via the process of sodium borohydride reduction. For comparison, non-radioactive silver nanoparticles were synthesised using commercially supplied AgNO3 in order to check if the use of irradiated Ag powder as a starting material would alter in any way the final nanoparticle characteristics. Both nanoparticle types were characterised using dynamic light scattering, zeta-potential and X-ray diffraction measurements, while additionally the non-radioactive samples were analysed by transmission electron microscopy and UV–Vis spectrometry. A hydrodynamic diameter of about 16 nm was determined for both radiolabelled and non-radioactive nanoparticles, while the electron microscopy on the non-radioactive samples indicated that the physical size of the metal NPs was (7.3 ± 1.4) nm.This study has been partially supported by the European Commission’s 7th Framework Programme project QualityNano under the contract agreement SP4-CAPACITIES- 2010-262163.Peer Reviewe

Two year evolution of radon emission and tectonic movements in Tuzla Fault, Seferihisar-Izmir

WOS: 000333795900017PubMed ID: 24530978Previous studies showed that tectonic movement and meteorological events are accompanied with radon release. In this study soil gas radon and tectonic movements were investigated with meteorological factors taking into account. Soil gas radon measurements were collected over a-two year period (2008-2010) at the Tuzla Fault in Turkey. The relationship between radon anomalies and seismic activities was evaluated using Dobrovolsky's form. It is shown that not only magnitude of earthquake but also distance from the measurement site should be used for identifying radon anomalies. (C) 2014 Elsevier Ltd. All rights reserved

Radiolabeled QDots: As a new multifunctional liquid penetrant solvent

European Federation for Non-Destructive Testing (EFNDT);Slovenian Society for Non-Destructive testing (SSNDT);TEAM TRADE d.o.o.14th International Conference of the Slovenian Society for Non-Destructive Testing: Application of Contemporary Non-Destructive Testing in Engineering -- 4 September 2017 through 6 September 2017 -- 136172Liquid Penetrant Technique (PT) is a sensitive non-destructive testing (NDT) for detecting and locating the presence of surface cracks. In this study, we present usability of radiolabeled quantum dots (Qdots) (CdSe/ZnS) with high fluorescence properties and radio tracer, as a new multifunctional penetrant for NDT applications. In order to inspect the flaws in weld defects, synthesized radiolabeled QDots were applied to the test piece and then counted the radioactivity measurements by using CdTe detector. The data obtained from the weld defect sample were analysed and interpreted. The results showed that higher radioactivity level was observed around the crack region which is consistent with the sample NDT inspection report. © 2017 Slovenian Society for Non-Destructive Testing. All rights reserved

Polymer coated iron nanoparticles: Radiolabeling & in vitro studies

PubMed: 323511922-s2.0-85103993228Background: Superparamagnetic iron oxide nanoparticles (SPIONs) have been extensively used for targeted drug delivery systems due to their unique magnetic properties. Objective: In this study, it has been aimed to develop a novel targeted99mTc radiolabeled polymeric drug delivery system for Gemcitabine (GEM). Methods: Gemcitabine, an anticancer agent, was encapsulated into polymer nanoparticles (PLGA) together with iron oxide nanoparticles via double emulsion technique and then labeled with99mTc. SPIONs were synthesized by reduction–coprecipitation method and encapsulated with oleic acid for surface modification. Size distribution and the morphology of the synthesized nanoparticles were characterized by dynamic light scattering (DLS) and scanning electron microscopy (SEM), respectively. The radiolabeling yield of SPION-PLGAGEM nanoparticles was determined via Thin Layer Radio Chromatography (TLRC). Cytotoxicity of GEM loaded SPION-PLGA was investigated on MDA-MB-231 and MCF7 breast cancer cells in vitro. Results: SEM images displayed that the average size of the drug-free nanoparticles was 40 nm and the size of the drug-loaded nanoparticles was 50 nm. The diameter of nanoparticles was determined as 366.6 nm by DLS, while zeta potential was found as 29 mV. SPION was successfully coated with PLGA, which was confirmed by FTIR. GEM encapsulation efficiency of SPION-PLGA was calculated as 4±0.16% by means of HPLC. Radiolabeling yield of SPION-PLGA-GEM nanoparticles was determined as 97.8±1.75% via TLRC. Cytotoxicity of GEM loaded SPION-PLGA was investigated on MDA-MB-231 and MCF7 breast cancer cells. SPION-PLGA-GEM showed high uptake on MCF-7, while the incorporation rate was increased for both cell lines with external magnetic field application. Conclusion:99mTc labeled SPION-PLGA nanoparticles loaded with GEM may overcome some of the obstacles in anti-cancer drug delivery because of their appropriate size, non-toxic, and superparamagnetic characteristics. © 2021 Bentham Science Publishers.Celal Bayar University Scientific Research Projects Co-ordination Unit Grant Number: 2017-00

Synthesis And Radiolabeling Of Temozolomide Loaded Solid Lipid Nanoparticles

WOS: 00045501940201

Effects of broccoli extract on biodistribution and labeling blood components with 99mTc-GH [Efeitos do extrato de brócolis na biodistribuição e marcação dos componentes do sangue com 99mTc-GH]

PubMed ID: 21952655Purpose: People consume vegetables without the knowledge of the side effects of the biological and chemical contents and interactions between radiopharmaceuticals and herbal extract. To this end, current study is focused on the effects of broccoli extract on biodistribution of radiolabeled glucoheptonate (99mTc-GH) and radiolabeling of blood components. Methods: GH was labeled with 99mTc. Quality control studies were done utilizing TLC method. Biodistribution studies were performed on male rats which were treated via gavage with either broccoli extract or SF as control group for 15 days. Blood samples were withdrawn from rats' heart. Radiolabeling of blood constituents performed incubating with GH, SnCl2 and 99m Tc. Result s: Radiochemical yield of 99mTc-GH is 98.46±1.48 % (n=8). Biodistribution studies have shown that according to the control, the treated group with broccoli has approximately 10 times less uptake in kidney. The percentage of the radioactivity ratios of the blood components is found to be same in both groups. Conclusions: Although there is no considerable effect on the radiolabeling of blood components, there is an outstanding change on the biodistribution studies especially on kidneys. The knowledge of this change on kidney uptake may contribute to reduce the risk of misdiagnosis and/or repetition of the examinations in Nuclear Medicine

Preparation of technetium-99m labeled ibuprofen by direct route and technetium-99m tricarbonyl route: a comparison of in vivo behaviors

PubMed: 324791912-s2.0-85087036506In the current study, ibuprofen (ibu) which is a non-steroidal anti-inflammatory drug (NSAID) was radiolabeled with 99mTc using two different methods: stannous chloride method (direct route) and technetium-99m tricarbonyl [99mTc(CO)3]+ route. Thus, it’s aimed to investigate the radiolabeling potential of ibu for inflammation detection and to monitor if there is any difference in in vivo distribution depending on the radiolabeling route. Quality control studies of both radiolabeled ibu were performed by radiochromatographic methods (Thin Layer Liquid Radio Chromatography and High Performance Liquid Radio Chromatography). Radiolabeling yields of 99mTc-ibu and 99mTc(CO)3-ibu were determined as 99.05 ± 0.83% and 91.79 ± 3.30% (n = 5), respectively. Experimental lipophilicities of both radiolabeled ibu were determined. The biological behavior of both radiolabeled ibu was investigated in healthy Albino Wistar male rats by in vivo biodistribution studies. It was seen that both radiolabeled ibuprofen showed renal excretion while organ uptakes of 99mTc-ibu and 99mTc(CO)3-ibu differ against time. © 2020, © 2020 Informa UK Limited, trading as Taylor & Francis Group.Ege Üniversitesi: 2011 NBE 010Present study was financially supported by the Ege University Scientific Research Project numbered 2011 NBE 010 (Bornova-Izmir, Turkey)