Phytochemical studies, antioxidant activity and total phenolic content of Ferula communis L. organ extracts

The present study aimed to assess antioxidant activities of three organs (flower, fruit and stem) extracts of Tunisian Ferula (F.). Various experimental models were used for the characterization of antioxidant activities, in vitro and using dichlorofluorescein (DCF) induced fluorescence techniques from phorbol myristate acetate (PMA ) stimulated human myeloid cell line HL-60communis. Results showed that the antioxidant activities varied considerably with organs. Thus, flower exhibited higher DPPH• scavenging ability, reducing and chelating power than stem and fruit. Moreover antioxidant capacities using ORAC method and a cell based-assay showed that fruit and stem exhibited statistically similar antioxidant activities. Moreover, F. communis contains high amounts of flavonoids with various health benefits as antioxidant properties attributed to their antioxidant potential. Likewise, to obtain biologically relevant information, the antioxidant activities of the extracts were evaluated on cellular models implicating the antioxidant activities; this test generally showed that F. communis flower extracts have the highest antioxidant capacities correlated to the highest total phenolic content. The identification of phenolic compounds in F. communis extracts using RP-HPLC revealed that resorcinol, ferulic and syringic acids together with coumarin were the major molecules

Comparative assessment of phytochemical profiles and antioxidant properties of Tunisian and Egyptian anise (Pimpinella anisum L.) seeds

Anis (Pimpinella anisum L.) seeds obtained from two geographic origins Tunisia (TAS) and Egypte (EAS) were studied regarding their biochemical composition and the antioxidant potential of their extracts. The results showed that the highest value of oil was detected with TAS compared to that of EAS ones. Ten (10) fatty acids were identified for the two locations and petroselinic acid was the most prevalent in oil seeds and interestingly, TAS displayed a significantly higher level of this acid than EAS. Besides, TAS exhibited slightly higher essential oil yield than the Egyptian variety and that trans-anethole was the dominant for the two provenances. In both accessions, the highest total phenolic, flavonoid and tannin content was obtained with ethyl acetate fraction. Therefore, TAS exhibited higher chelating and reducing powers than EAS which may be due to a slightly different phenolic composition between the two accession seed extracts. The phenolic compositions of TAS and EAS revealed that ethyl acetate extracts showed higher proportions of naringin, chlorogenic acid and rosmarinic acid. However, ethanol extracts were richer in larcitrin, rosmarinic acid and cirsimartin. The overall results revealed that aniseeds might constitute a novel source of natural antioxidants and could be used as food additive

Tetraclinis articulata essential oil emulsion use as alternative to chemical fungicide to control tomato grey mould disease

Tetraclinis articulata essential oil proved to be effective in controlling tomato grey mould, so we would investigate its effect on some tomato defense mechanisms. The pretreatment of Botrytis cinerea infected tomato plants with TAEO emulsion enhanced the activity of antioxidant enzymes activity SOD, CAT, APX, and GPX, and total polyphenols content and it decreased IC50 of free radical-scavenging activity and H2O2 content. Results showed amelioration in antioxidant status in TAEO emulsion treated and B. cinerea infected plants indicating that treatment decreased infection in tomato plants. The qRT-PCR analysis of defense genes expression Chitinase SlChi, transcription factors SlWRKY and SlAP2/ERF, Lipoxygenase SlLOX, and Thioredoxin SlTRX showed that they were up-regulated as early as 12 hpi sustained with a second increase at 48 hpi in TAEO emulsion pretreated and infected plants. These results suggest the potential use of TAEO emulsion as natural product to induce tomato antioxidant status and activate defense genes

Towards the use of Cupressus sempervirens L. organic extracts as a source of antioxidant, antibacterial and antileishmanial biomolecules

Cupressus sempervirens L. is largely used in traditional medicine as an antimicrobial agent. The present study investigated the antioxidant, antibacterial and antileishmanial activities of C. sempervirens organic extracts at different phenological stages. Antioxidant activity was determined by DPPH (2,2-diphenyl-1-picrylhydrazylradical) scavenging assay, ferric reducing power and total antioxidant capacity. The antibacterial activity was evaluated against five clinical strains by disk diffusion method and minimum inhibitory concentration (MIC). The antileishmanial activity was determined against promastigote and amastigote forms of Leishmania (L.) infantum and L. major. Results of antioxidant activity showed that methanolic extract from vegetative stage had the most important activity. The ethyl acetate extract of C. sempervirens from flowering stage was the most active against Bacillus cereus (ATCC 14579) and Staphylococcus aureus (ATCC 25923) with MIC of 100 μg/mL and 50 μg/mL, respectively. Interestingly, this extract exhibited high antileishmanial activity against promastigote form of L. infantum and L. major (IC50 = 1.47 and 2.8 μg/mL, respectively) and amastigote form (IC50 = 3.61 and 5.42 μg/mL, respectively). Furthermore, ethyl acetate extract showed low cytotoxicity on macrophage cells Raw264.7 with selectivity index of 34.15 and 17.93 for L. infantum and L. major, respectively. The identification by HPLC and HPLC-MSn of active extracts of C. sempervirens revealed that major compounds of methanolic extract from vegetative stage and ethyl acetate extract from flowering stage were cupressuflavone and amentoflavone. Based on these results, C. sempervirens extracts could be used as an alternative to chemical drugs for the treatment of oxidative stress and infectious diseases

Phytochemical composition and antioxidant activities of different aerial parts extracts of Ferula communis

peer reviewedThe present study aimed to assess antioxidant activities of three organs (flower, fruit, and stem) extracts of Tunisian Ferula (F.) communis. Various experimental models were used to characterize the antioxidant activities in vitro as well as on ROS-induced fluorescence using dichlorofluorescein technique from phorbol myristate acetate (PMA)-stimulated human myeloid cell line HL-60. Results showed that the antioxidant activities varied considerably with organs. Thus, flower exhibited higher DPPH-scavenging ability, reducing and chelating power than stem and fruit. Also, antioxidant capacities using ORAC method and a cell-based assay showed that fruit and stem exhibited statistically similar antioxidant activities. Moreover, F. communis contains high amounts of flavonoids with various health benefits attributed to their antioxidant potential. Likewise, to obtain biologically relevant information, the antioxidant activities of the extracts were evaluated on cellular models implicating the antioxidant activities; this test generally showed that F. communis flower extracts have the highest antioxidant capacities correlated to the highest total phenolic content. The identification of phenolic compounds in F. communis extracts using RP-HPLC revealed that resorcinol, ferulic, and syringic acids together with coumarin were the major molecules. © 2018 Società Botanica Italian

Phytochemical composition and antioxidant activities of different aerial parts extracts of <i>Ferula communis</i> L.

<p>The present study aimed to assess antioxidant activities of three organs (flower, fruit, and stem) extracts of Tunisian <i>Ferula (F.) communis.</i> Various experimental models were used to characterize the antioxidant activities <i>in vitro</i> as well as on ROS-induced fluorescence using dichlorofluorescein technique from phorbol myristate acetate (PMA)-stimulated human myeloid cell line HL-60. Results showed that the antioxidant activities varied considerably with organs. Thus, flower exhibited higher DPPH-scavenging ability, reducing and chelating power than stem and fruit. Also, antioxidant capacities using ORAC method and a cell-based assay showed that fruit and stem exhibited statistically similar antioxidant activities. Moreover<i>, F. communis</i> contains high amounts of flavonoids with various health benefits attributed to their antioxidant potential. Likewise, to obtain biologically relevant information, the antioxidant activities of the extracts were evaluated on cellular models implicating the antioxidant activities; this test generally showed that <i>F. communis</i> flower extracts have the highest antioxidant capacities correlated to the highest total phenolic content. The identification of phenolic compounds in <i>F. communis</i> extracts using RP-HPLC revealed that resorcinol, ferulic, and syringic acids together with coumarin were the major molecules.</p