Both completeness and errors were larger in academic journals.

<p>Completeness (A) and errors (B) for each of the categories of written press. Each dot represents one article. Significance determined by ANOVA, ****—p = <0.0001, ***—p = <0.001, **—p = <0.01, *—p = <0.05, f = p value significant if outlier statistically removed from academic errors group.</p

Completeness and accuracy scores by media type.

<p>Aggregate scores for coverage of topics were collected for each media type based on individual media outlets (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0179356#pone.0179356.s001" target="_blank">S1 Table</a>). Nations impacted in the 2013–2016 outbreak were not counted against those sources that pre-dated the outbreak.</p

Completeness and errors did not correlate to markers of journal quality.

<p>Each academic press article is graphed for completeness versus impact factor (A), errors versus impact factor (B), completeness versus authors (C) and citations (D). (E) Completeness for full open access journals versus limited access is shown, red dots indicate articles that were free of errors. Errors versus authors (F), citations (G), and pages (H) are shown. (I) completeness versus page length is shown.</p

The Crisis and Emergency Risk Communication (CERC) model for topic scoring.

<p>The CERC model was used to generate topic lists for scoring of accuracy, completeness, as well as enumerating unrelated information.</p

Systematic review inclusion process for academic articles focusing on general review.

<p>(A) The primary search for articles written after the Western African outbreak. (B) A similar search for general topic review articles from 2010 up until the Western African outbreak onset.</p

The resultant scores for comprehensive review articles under Crisis and Emergency Risk Communication (CERC) model.

<p>Scores for presence or absence of covered topics were generated using the CERC model in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0179356#pone.0179356.t001" target="_blank">Table 1</a>. Citations 111–114 pre-date 2013 EVD outbreak. Red indicates the citation covered the topic, blue indicates the topic was not covered, and grey indicates not applicable.</p

Fatty acid content and source for the diets studied.

<p>Breakdown of dietary components in the diets studied are shown, including protein, carbohydrates (carb), fat, and % of fat that was saturated, poly-unsaturated fatty acids (PUFA), or mono-unsaturated fatty acids (MUFA). The dietary source for each fatty acid is shown. Both diets were derived from natural oils. Human recommended diet (RD) reflects the guidelines of the United States Department of Agriculture. See Methods section for further details on diet formulation.</p

Summary of study design.

<p>(a) For experiments evaluating the effects of parental diet, littermate mice were placed on either Low Fat or high omega-3 formulations one day prior to being placed in breeding cages. Breeder mice were maintained on the different diets throughout gestation and nursing. When the pups were three weeks post-partum, they were weaned to new cages. All pups were weaned onto the Low Fat control diet. Two to four weeks after weaning, the mice were evaluated in the described models. (b) For evaluation of the effects of active diet consumption, the converse experiment was performed. Pups from breeders on the Low Fat control diet were weaned into new cages and placed on either the omega-3 or Low Fat control diet. Figure modified from <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0087181#pone.0087181-Myles1" target="_blank">[13]</a>.</p

Omega-3 consumption altered MRSA susceptibility.

<p>(a) Survival after infection with <i>E. coli</i> 01 K18 in pups of breeders exposed to LF or n−3 diet (n = 10–12). (b–g) <i>Staphylococcus aureus</i> (MRSA USA300) skin infection in male BALB/c mice. Lesion sizes (b), CFU (c), and mRNA expression in skin abscess tissue normalized against LF controls (dotted line) (d) (n = 5–6) in offspring mice. Lesion sizes (e), CFU (f), and mRNA expression in skin abscess tissue normalized against LF controls (dotted line) (g) (n = 10) in adult male mice. Results are representative of two (d and g) or combined from 2–3 (a–c and e–f) independent experiments and displayed as mean+s.e.m. Significance determined by t test (b–c, e–f), ANOVA with Bonferroni’s correction (d and g), or Kaplan-Meier (a). n designates mouse number per group.</p

High omega-3 intake altered colonic inflammation and gut microbiome.

<p>(a) 16S ribosomal RNA genes in cecal stool samples of female mice. Each bar represents one mouse. Phyla (Bacterioidetes, Firmicutes, or other) are classified into genus or unclassified family members. Further breakdown of composition within each phylum can be found in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0087181#pone-0087181-t002" target="_blank">Table 2</a>. (b-f) Cytokine production from female excised colons stimulated with LPS for 24–72 hours (n = 4–6). (g) Cytokine production from male splenocytes stimulated with LPS for 24–72 hours (n = 4–6). Results are representative of 2–3 independent experiments in BALB/c mice and displayed as mean+s.e.m. Significance determined by t test. All experiments were repeated with similar results in both genders. Gender is indicated when representative experiments are shown; otherwise, data reflects both male and female mice with matched ratios within experiments. n designates number of mice per experiment. The results from the Low Fat group shown in (a) are a subset of what was previously reported in reference 13. The remaining data is novel.</p