Mountain Lion (\u3ci\u3ePuma concolor\u3c/i\u3e) and Feral Horse (\u3ci\u3eEquus ferus\u3c/i\u3e) Interactions: Examining the Influence of a Non-Native Ungulate on Predator Behavior in a Semi-Arid Environment

A non-native is a species that evolved in one ecosystem and has established within another. Non-native species can thrive in new ecosystems as they can spread quickly, outcompete and replace native species, and disrupt food webs. Domestic horses were brought to North America by Europeans in 1493. They are now found in 11 states across the western United States. Feral horses can negatively impact vegetation, endangered species habitat, compete with native species, and be important prey for mountain lions under certain conditions. But do feral horses affect the food web? Feral horses are managed by large removals to reduce the population, and therefore this food source can quickly decrease. I addressed two questions in Southeast, NV where, a horse removal occurred in December 2020 (455 horses; 45% of the population): (1) following removals, do mountain lions increase predation on native species, such as mule deer or bighorn sheep? and (2) Do mountain lions select feral horses over native species? I hypothesized: (1) horses will decrease from the diet and native prey will increase, and (2) mountain lions will select for native prey over feral horses. I deployed 49 cameras to determine prey availability and investigated 1,360 potential kill sites from 29 GPS-collared mountain lions. Prey was found at 1,056 sites. To test if mountain lions preyed more native species post-removal, I compared their diet in the years pre- and post-removal. I found that the diet did not change between years, indicating that they consumed horses and native prey similarly. To test if mountain lions were selecting for native prey, I compared each mountain lion\u27s diet to their prey availability. The results showed mountain lions selected for horses during the growing season, while another two selected for deer during winter. Overall, feral horses made-up about 36% of the diet, with some individuals consuming horses more than others. In total, 342 horses were consumed by mountain lions during this study. I concluded that feral horse presence influences mountain lion predation behaviors, which is important for mountain lion and feral horse management in arid ecosystems that have many prey specie

Single prazosin infusion in prelimbic cortex Fosters extinction of amphetamine-induced conditioned place preference

Exposure to drug-associated cues to induce extinction is a useful strategy to contrast cue-induced drug seeking. Norepinephrine (NE) transmission in medial prefrontal cortex has a role in the acquisition and extinction of conditioned place preference induced by amphetamine. We have reported recently that NE in prelimbic cortex delays extinction of amphetamine-induced conditioned place preference (CPP). A potential involvement of α1-adrenergic receptors in the extinction of appetitive conditioned response has been also suggested, although their role in prelimbic cortex has not been yet fully investigated. Here, we investigated the effects of the α1-adrenergic receptor antagonist prazosin infusion in the prelimbic cortex of C57BL/6J mice on expression and extinction of amphetamine-induced CPP. Acute prelimbic prazosin did not affect expression of amphetamine-induced CPP on the day of infusion, while in subsequent days it produced a clear-cut advance of extinction of preference for the compartment previously paired with amphetamine (Conditioned stimulus, CS). Moreover, prazosin-treated mice that had extinguished CS preference showed increased mRNA expression of brain-derived neurotrophic factor (BDNF) and post-synaptic density 95 (PSD-95) in the nucleus accumbens shell or core, respectively, thus suggesting that prelimbic α1-adrenergic receptor blockade triggers neural adaptations in subcortical areas that could contribute to the extinction of cue-induced drug-seeking behavior. These results show that the pharmacological blockade of α1-adrenergic receptors in prelimbic cortex by a single infusion is able to induce extinction of amphetamine-induced CPP long before control (vehicle) animals, an effect depending on contingent exposure to retrieval, since if infused far from or after reactivation it did not affect preference. Moreover, they suggest strongly that the behavioral effects depend on post-treatment neuroplasticity changes in corticolimbic network, triggered by a possible “priming” effect of prazosin, and point to a potential therapeutic power of the antagonist for maladaptive memories

Knowledge Barriers to Diffusion of Telemedicine

Telemedicine is one proposed solution to problems of accessibility, quality, and costs of medical care. Although telemedicine applications have proliferated in recent years, their diffusion has remained low in terms of the volume of consultations. In this study, Attewell’s (1992) theory of knowledge barriers is extended to explain why diffusion of telemedicine remains low. In case studies of telemedicine programs in three world- renowned medical centers in Boston, Massachusetts, we find that, in addition to technical knowledge barriers, as suggested by Attewell, there are economic, organizational, and behavioral knowledge barriers that inhibit the diffusion of telemedicine. The lowering of these barriers entails intensive learning efforts by proponents of applications within adopter organizations. They need to develop technically feasible, medically valid, reimbursable, and institutionally supported applications in order to justify the value of telemedicine and engender frequent and consistent use by physicians

Entrepreneur Or Intermediary: The Nature of the Relationship Manager\u27s Job

A new liaison role between Information Systems (IS) and users, the relationship manager (RM), has recently emerged.Accordingtotheprescriptiveliterature,RMsaddvaluebydeepunderstanding ofthebusinessestheyserve and technologyleadership. Little is known, however, about their actual work practices. Is the RM an intermediary, filtering information and sometimes misinformation, from clients to IS, or do they play more pivotal roles as entrepreneurs and change agents? This article addresses these questions by studying four RMs in four different industries. The RMs were studied using the structured observation methodology employed by Mintzberg (CEOs), Ives and Olson (MIS managers), and Stephens et al. (CIOs). The findings suggest that while RMs spend less time communicating with users than one would expect, they are leaders, often mavericks, in the entrepreneurial work practices necessary to build partnerships with clients and to make the IS infrastructure more responsive to client needs

Bridging the IS-Line Interface: The Role of the Relationship Manager

IS Organizations are increasingly focusing on managing the interface between themselves and their clients within organizations to improve not only the level of customer service but also the utilization of firm investments in hardware and software. Several organizations have recently created full-time specialized positions, often termed \u27Relationship Manager\u27 to manage the relationship between IS and Line groups. This paper presents the results of an exploratory study to understand the role of the \u27Relationship Manager\u27(RM) and how people in these positions deliver value to IS and Line groups in the organization. Our findings are that the role of the RMs is complex, requiring a focus on the coordination of activities across the IS-Line interface in the short run while marshaling organizational energy towards initiatives that position the firm favorably in the medium and long term. Combining an intimate knowledge of the organization with expertise in IT, the RMs utilize their influential positions in the informal network within the firm to create and capitalize on opportunities to champion innovation and enable change

Riccati-parameter solutions of nonlinear second-order ODEs

It has been proven by Rosu and Cornejo-Perez in 2005 that for some nonlinear second-order ODEs it is a very simple task to find one particular solution once the nonlinear equation is factorized with the use of two first-order differential operators. Here, it is shown that an interesting class of parametric solutions is easy to obtain if the proposed factorization has a particular form, which happily turns out to be the case in many problems of physical interest. The method that we exemplify with a few explicitly solved cases consists in using the general solution of the Riccati equation, which contributes with one parameter to this class of parametric solutions. For these nonlinear cases, the Riccati parameter serves as a `growth' parameter from the trivial null solution up to the particular solution found through the factorization procedureComment: 5 pages, 3 figures, change of title and more tex

HyFlex EDM rotary Ni-Ti prototypes: the effect of an innovative machining technology on Ni-Ti wear

Aim. HyFlex EDM files were recently introduced pre- senting an innovative electro discharge machining (EDM) process of fabrication. The aim of this study was to evalua- te the surface and microstructural alterations of new and in vitro used HyFlex EDM Ni-Ti rotary prototypes. Methods. The surface and microstructural characteristi- cs of 15 new HyFlex EDM instruments were analyzed by ESEM equipped with energy dispersive x-ray spectropho- tometry (EDS) and optical metallographic imaging. Instru- ments were subjected to instrumentation tests on severely curved root canals (ranging between 50° and 70°) of ex- tracted multi-rooted teeth. Once that canal patency was verified with a #10 K-file, the working length was determi- ned by subtracting 1mm. HyFlex EDM files were used with a 16:1 reduction handpiece X-Smart (Dentsply Maillefer, Baillagues, Switzerland) following the manufacture’s direc- tion, at 500 rpm and 2.5Ncm, with slightly apical pressure and pecking motion. The operative sequence was: 25/12 at 2/3 of the WL, 10/05 and 25/08 at WL. Irrigation was per- formed at every change of instrument, with a total amount of3mlof5%NaOCland3mlof10%EDTA(Ogna,Mug- giò, Italy). Each instrument was used in 10 curved canals, washed in an ultrasonic bath containing detergent for 10 min and then autoclaved at 134°C. Surface and microstruc- tural characterizations were repeated on used instruments at same points and with same angulations to compare the pre- and postoperative micrographs, in order to verify the appearance of fractures, unwinding, microcracks, blade di- sruption and tip deformation. Results. Surface and microstructural characteriza- tion of new instruments revealed the typical features of a NiTi ED-Machined alloy with an irregular and “craters-like” surface. High magnification microgra- phs disclosed a non-uniform structure were pits, po- res and voids caused the peculiar aspect of a “rough- spark-machined” surface. No fractures were registered during instrumentation of curved canals. Surface and microstructural characterization of used files revealed no wear and no degradation of the 25.12 and 25.08 files. The tip segment was confirmed as the most me- chanically stressed portion of 10.05 prototypes. All the instruments, after several uses, well-preserved the “craters-like” irregular surface without cutting edge al- terations. The metallographic inspection on the cross section of brand new HyFlex EDM files showed an homogeneous martensitic phase. The microstructure appeared uniform from the surface to the bulk, and no microcracks or defect were identified, even at high optical magnification (1000X). Conclusion. Unaltered spark-machined surface and low microstructural degradation are the main features of recently introduced HyFlex EDM. Caution would be re- commended regarding reuse of small HyFlex EDM files. Instruments exhibited a safe in vitro use in presence of severely curved canals

Unilateral spatial neglect after stroke: Current insights

INTRODUCTION: Unilateral spatial neglect (USN) is a disorder of contralesional space awareness which often follows unilateral brain lesion. Since USN impairs awareness of contralesional space/body and often of concomitant motor disorders, its presence represents a negative prognostic factor of functional recovery. Thus, the disorder needs to be carefully diagnosed and treated. Here, we attempted to present a clear and concise picture of current insights in the comprehension and rehabilitation of USN. METHODS: We first provided an updated overview of USN clinical and neuroanatomical features and then highlighted recent progresses in the diagnosis and rehabilitation of the disease. In relation to USN rehabilitation, we conducted a MEDLINE literature research on three of the most promising interventions for USN rehabilitation: prismatic adaptation (PA), non-invasive brain stimulation (NIBS), and virtual reality (VR). The identified studies were classified according to the strength of their methods. RESULTS: The last years have witnessed a relative decrement of interest in the study of neuropsychological disorders of spatial awareness in USN, but a relative increase in the study of potential interventions for its rehabilitation. Although optimal protocols still need to be defined, high-quality studies have demonstrated the efficacy of PA, TMS and tDCS interventions for the treatment of USN. In addition, preliminary investigations are suggesting the potentials of GVS and VR approaches for USN rehabilitation. CONCLUSION: Advancing neuropsychological and neuroscience tools to investigate USN pathophysiology is a necessary step to identify effective rehabilitation treatments and to foster our understanding of neurofunctional bases of spatial cognition in the healthy brain

SEDIMENTARY DYNAMICS OF THE MARETTIMO CONTINENTAL SHELF AND MARETTIMO CHANNEL (EGADI ISLANDS – WESTERN MEDITERRANEAN): ANALYSIS OF DEPOSITIONAL AND EROSIONAL FEATURES.

Sedimentary dynamics of the Marettimo continental shelf and Marettimo Channel (Egadi Island – Western Mediterranean), have been unveiled based on the erosive and depositional features observed at the seafloor. Different morphologic features were recognized through side scan sonar, multibeam and high-resolution seismics, acquired in the framework of national Research Projects (CARG, GebecSud, MaGIC) carried out by the Department of Earth and Marine Science (former Department of Geology and Geodesy) of the University of Palermo. Sediment samples have been also collected and analyzed. The Egadi Islands are part of the NW Sicilian offshore, and belong to the mountain chain that connects the Sicilian chain to the Maghrebides one. The Marettimo Island represents an isolated portion of the Egadi continental shelf and is separated from the rest of the archipelago by the Marettimo Channel. Previous studies allowed to recognize different kind of sedimentary structures along the seafloor. Lo Iacono and Guillén (2008) described extensive field of subaqueous dunes (Ashley, 1990) along the Marettimo inner shelf, forming on gravelly and pebbly sediments and related to strong storm events (H:5.5 m, T: 12 s). Larger dunes have been observed in the north-western sector of the inner shelf with wavelengths ranging from 1 to 2.5 m, heights between 15 cm and 30 cm and extending laterally for hundreds of meters. Moreover in the north-eastern sector of the inner shelf, sorted bedforms have been detected at a depth of 40 m: they are characterized by a width varying between 15 m and 50 m and a length that reaches hundreds of meters. In the south-eastern sector of the Marettimo outer shelf, Colantoni et al. (1993) map two groups of sedimentary structures. In the same area, Lo Iacono (2004) recognized two-dimensional and three-dimensional subaqueous dunes. 2D dunes are found at depths between 60 and 90 m and show a wavelength variable between 15 and 50 m. 3D dunes, which are found at a depth of 80 m, are characterized by a wavelength of 30 m and a lateral extent of 50 m. Morphological evidences of a strong hydrodynamic regime come also from the morphobathymetric analysis of the Marettimo Channel, that is a 24 km long submarine valley oriented NNW-SSE, with a depth ranging from a minimum of 180 m to a maximum of 370 m and a width from 2 km to 14 km. Inside the channel, south of its narrowest point, a 180 m deep bathymetric threshold separates two areas, which deepens towards opposite directions: north-west and south. The flanks of the sector that deepens towards the NW are affected by mass-wasting features (Lo Iacono et al., 2007). The western flank of the channel, which delimits the Marettimo continental shelf, is carved by gullies, making the shelf-edge very uneven. Gullies develop to a maximum depth of 280 m and have a length of 250 m and a width of 50 m. At the bottom of the channel a linear incision 20 m deep, 400 m wide and almost 3 km long, is probably generated by erosive bottom currents. On the whole, the morpho-sedimentary structures here described reveal a strong hydrodynamic regime. The morphological and textural features of the bedforms observed on the Marettimo inner continental shelf suggest the occurrence of sporadic medium to strong storm events coming from western sectors. The morphological characteristics of the Marettimo Channel reveal the occurrence of strong bottom currents probably related to the severe interchange of water masses between the Tyrrhenian Sea and the Sicily Channel. References: Ashley, G., 1990. Journal of Sedim. Petrology 60, 160–172. Colantoni, P., Ligi, M., Morsiani, M.P., Penitenti, D., 1993. UNESCO Reports in Mar. Science 58, 93–98. Lo Iacono, C., 2004. Università di Napoli ‘‘Federico II,’’ Naples, Italy. Ph.D. Thesis, 150pp... Lo Iacono, C., Catalano, R., Agate, M., 2007. VI Conv. FIST GeoItalia, Rimini (Italy), 12-14 Sept. Lo Iacono C., Guillén J., 2008. Continental Shelf Research 28, 245–256

Amino acid substitutions within the heptad repeat domain 1 of murine coronavirus spike protein restrict viral antigen spread in the central nervous system.

Targeted recombination was carried out to select mouse hepatitis viruses (MHVs) in a defined genetic background, containing an MHV-JHM spike gene encoding either three heptad repeat 1 (HR1) substitutions (Q1067H, Q1094H, and L1114R) or L1114R alone. The recombinant virus, which expresses spike with the three substitutions, was nonfusogenic at neutral pH. Its replication was significantly inhibited by lysosomotropic agents, and it was highly neuroattenuated in vivo. In contrast, the recombinant expressing spike with L1114R alone mediated cell-to-cell fusion at neutral pH and replicated efficiently despite the presence of lysosomotropic agents; however, it still caused only subclinical morbidity and no mortality in animals. Thus, both recombinant viruses were highly attenuated and expressed viral antigen which was restricted to the olfactory bulbs and was markedly absent from other regions of the brains at 5 days postinfection. These data demonstrate that amino acid substitutions, in particular L1114R, within HR1 of the JHM spike reduced the ability of MHV to spread in the central nervous system. Furthermore, the requirements for low pH for fusion and viral entry are not prerequisites for the highly attenuated phenotype