12 research outputs found
Immunoaffinity chromatography for the sample pretreatment of Taxus plant and cell extracts prior to analysis of taxanes by high-performance liquid chromatography
No full textPenyelesaian masalah pemotongan rol kertas dengan metode penghasil kolom
Get PDFThe application of immunoaffinity chromatography for the purification of Taxus plant and cell extracts prior to the HPLC analysis is described. Polyclonal antibodies raised against 10-deacetylbaccatin III (10-DAB III), paclitaxel's main precursor in plant, were characterised by enzymed-linked immunosorbent assay. Immunoglobulins from selected antisera were immobilised on CNBr-activated Sepharose 4B. The immunoaffinity column was used for the purification of plant and plant cell culture extracts prior to their analysis by HPLC. Immunoaffinity chromatography enabled the selective concentration of taxoids and enhanced sample clean-up. © 2002 Elsevier Science B.V. All rights reserved.SCOPUS: cp.jinfo:eu-repo/semantics/publishe
Verwendung der Iodid-bzw. Silber/Sulfid-selektiven Elektrode zur Bestimmung einiger tertiärer Amine und Alkaloide
No full textMicrodosing, isotopic labeling, radiotracers and metabolomics: relevance in drug discovery, development and safety
No full textGurum (Citrullus lanatus var. Colocynthoide) seed: lipid, amino acid, mineral, proximate, volatile compound, sugar, vitamin composition and functional properties
No full textSimultaneous Separation of Water- and Fat-Soluble Vitamins by Selective Comprehensive HILIC Ă— RPLC (High-Resolution Sampling) and Active Solvent Modulation
No full textExploiting biased reptation for continuous flow preparative DNA fractionation in a versatile microfluidic platform
No full textDevelopment of a New Methodology for Determination of Vitamin B9 at Trace Levels by Ultrasonic-Assisted Cloud Point Extraction Prior to HPLC
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