Human and Canine Echinococcosis Infection in Informal, Unlicensed Abattoirs in Lima, Peru

Echinococcus granulosus infections are a major public health problem in livestock-raising regions around the world. The life cycle of this tapeworm is sustained between dogs (definitive host, canine echinococcosis), and herbivores (intermediary host, cystic hydatid disease). Humans may also develop cystic hydatid disease. Echinococcosis is endemic in rural areas of Peru; nevertheless, its presence or the extension of the problem in urban areas is basically unknown. Migration into Lima, an 8-million habitant's metropolis, creates peripheral areas where animals brought from endemic areas are slaughtered without veterinary supervision. We identified eight informal, unlicensed abattoirs in a peripheral district of Lima and performed a cross-sectional study in to assess the prevalence of canine echinococcosis, evaluated by coproELISA followed by PCR evaluation and arecoline purge. Eight of 22 dogs (36%) were positive to coproELISA, and four (18%) were confirmed to be infected with E. granulosus tapeworms either by PCR or direct observation (purge). Later evaluation of the human population living in these abattoirs using abdominal ultrasound, chest X-rays and serology, found 3 out of 32 (9.3%) subjects with echinococcal cysts in the liver (two viable, one calcified), one of whom had also lung involvement and a strongly positive antibody response. Autochthonous transmission of E. granulosus is present in Lima. Informal, unlicensed abattoirs may be sources of infection to neighbouring people in this urban environment

Epidemiology of canine echinococcosis in northwest Libya, northwest Kenya (Turkana) and mid-Wales

Echinococcus granulosus is a cestode species responsible for human cystic echinococcosis (CE). The domestic dog is the primary definitive host for the transmission of E.granulosus infection to domestic livestock intermediate hosts and to humans. In order to determine the prevalence and risk factors for canine echinococcosis, epidemiological studies were conducted in known endemic localities in three different countries i.e. Libya, Kenya and Wales. Stray dogs were subject to direct examination of the small intestine at necropsy. Owned dogs (total n=1659) were screened for E.granulosus infection using a genus specific coproantigen ELISA test that was re- standardised against 75 dog necropsy results, and exhibited 100% sensitivity and 98% specificity. Analysis of risk factors for canine echinococcosis associated with Echinococcus coproantigen positive results was evaluated based on dog-owner questionnaires. In Libya, the overall infection of E.granulosus in autopsied dogs was 25.8%, and an overall coproantigen prevalence of 21.6% was obtained for owned dogs (n= 334). Risk factors (p4month intervals) of dogs. The data are discussed in relation to a previous pilot hydatid control program in that area of Wales (1983-89) and the potential for increase in transmission to humans. Identification of risk factors associated with canine echinococcosis appear to be similar in all three communities studied and help to demonstrate practices that may be amenable to change as part of hydatid control programmes