22 research outputs found

    EPR spectroscopy of <sup>53</sup>Cr monoisotopic impurity ions in a single crystal of yttrium orthosilicate Y<inf>2</inf>SiO<inf>5</inf>

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    Β© 2020 Elsevier B.V. The electron paramagnetic resonance spectra of 53Cr monoisotopic impurity ions in yttrium orthosilicate single crystals (Y2SiO5) were studied by electron paramagnetic resonance (EPR) in the X-band. The directions of the principal magnetic axes and the parameters of the effective spin Hamiltonian, which describes magnetic characteristics of the impurity centers of chromium are determined. It is shown that the orientation dependencies of the EPR spectra are described by the second-order spin Hamiltonian corresponding to the rhombic symmetry of the local crystal field acting on the impurity ion. It was assumed that the g-tensor and A-tensor describing the Zeeman energy of electron levels in a magnetic field and the hyperfine interaction of electron and nuclear spins are isotropic, and the entire anisotropy of the EPR spectra is due to the anisotropy of the D-tensor, which describes the fine structure of electron levels in crystal electric field. A strong dependence of the probability of forbidden transitions between hyperfine sublevels of electron levels on the orientation of an external magnetic field is established. Moreover, for some orientations, the probability of forbidden transitions becomes comparable to the probability of allowed transitions

    Iron oxidation state in La<inf>0.7</inf>Sr<inf>1.3</inf>Fe<inf>0.7</inf>Ti<inf>0.3</inf>O<inf>4</inf> and La<inf>0.5</inf>Sr<inf>1.5</inf>Fe<inf>0.5</inf>Ti<inf>0.5</inf>O<inf>4</inf> layered perovskites: Magnetic properties

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    La0.5Sr1.5Fe0.5Ti0.5O4 and La0.7Sr1.3Fe0.7Ti0.3O4 solid solutions with the layered perovskite structure were synthesized using a solid state method. Structural properties of obtained samples were characterized using X-ray diffraction and X-ray fluorescence analyses. Magnetic properties were investigated using magnetometry, electron spin resonance (ESR) and MΓΆssbauer spectroscopy methods. Based on magnetization and ESR measurements it was suggested the presence of Fe4+ ions in addition to trivalent iron ions that was exactly confirmed by MΓΆssbauer spectroscopy investigations. Based on all experimental results one can suggest the presence of the electronic phase separation in the investigated samples – the simultaneous existence of the paramagnetic phase and magnetically correlated regions, which form due to the mixed-valence iron ions. So the paramagnetic phase with strong antiferromagnetic correlation exists in both samples, while the second phase is ferromagnetically and ferrimagnetically correlated regions in La0.5Sr1.5Fe0.5Ti0.5O4 and La0.7Sr1.3Fe0.7Ti0.3O4, respectively

    Π₯арактСристика ΠΈΠΌΠΌΡƒΠ½ΠΎΡ„Π΅Π½ΠΎΡ‚ΠΈΠΏΠ° Ρ€Π΅Π·ΠΈΠ΄Π΅Π½Ρ‚Π½Ρ‹Ρ… ΠΌΠ°ΠΊΡ€ΠΎΡ„Π°Π³ΠΎΠ² ΠΏΠ΅Ρ‡Π΅Π½ΠΈ ΠΈ профиля экспрСссируСмых Π³Π΅Π½ΠΎΠ²

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    The profile of the expressed genes, phenotype and phagocytic activity of resident macrophages of the liver were studied. It was found that most of the resident liver macrophages were F4/80+, CD68+, CD163+, and CD11b-. Induction of М2 factor phenotypes increased CD86 expression as well as triggered ΠšΠ†67 expression. After activation of Ml and М2 by inducers, resident liver macrophages enhanced the expression of main anti-inflammatory cytokines. Resident liver macrophages showed pronounced phagocytic activity, which faded up to 5 hours after the administration of latex particles, but the addition of inducers of both the Ml and М2 phenotype stimulated phagocytosis during 24 hours.Π˜Π·ΡƒΡ‡Π΅Π½Ρ‹ ΠΏΡ€ΠΎΡ„ΠΈΠ»ΡŒ экспрСссируСмых Π³Π΅Π½ΠΎΠ² Ρ†ΠΈΡ‚ΠΎΠΊΠΈΠ½ΠΎΠ² ΠΈ ΠΌΠ°Ρ€ΠΊΠ΅Ρ€ΠΎΠ² ΠΌΠ°ΠΊΡ€ΠΎΡ„Π°Π³ΠΎΠ², Ρ„Π΅Π½ΠΎΡ‚ΠΈΠΏ ΠΈ фагоцитарная Π°ΠΊΡ‚ΠΈΠ²Π½ΠΎΡΡ‚ΡŒ Ρ€Π΅Π·ΠΈΠ΄Π΅Π½Ρ‚Π½Ρ‹Ρ… ΠΌΠ°ΠΊΡ€ΠΎΡ„Π°Π³ΠΎΠ² ΠΏΠ΅Ρ‡Π΅Π½ΠΈ. ΠžΠ±Π½Π°Ρ€ΡƒΠΆΠ΅Π½ΠΎ, Ρ‡Ρ‚ΠΎ большая Ρ‡Π°ΡΡ‚ΡŒ Ρ€Π΅Π·ΠΈΠ΄Π΅Π½Ρ‚Π½Ρ‹Ρ… ΠΌΠ°ΠΊΡ€ΠΎΡ„Π°Π³ΠΎΠ² ΠΏΠ΅Ρ‡Π΅Π½ΠΈ ΠΈΠΌΠ΅Π»Π° Ρ„Π΅Π½ΠΎΡ‚ΠΈΠΏ F4/80+, CD68+, CD163+, CD lib". ВоздСйствиС Ρ„Π°ΠΊΡ‚ΠΎΡ€ΠΎΠ², ΠΏΠΎΠ»ΡΡ€ΠΈΠ·ΡƒΡŽΡ‰ΠΈΡ… ΠΌΠ°ΠΊΡ€ΠΎΡ„Π°Π³ΠΈ Π² Π½Π°ΠΏΡ€Π°Π²Π»Π΅Π½ΠΈΠΈ М2-Ρ„Π΅Π½ΠΎΡ‚ΠΈΠΏΠ°, ΠΏΡ€ΠΈΠ²ΠΎΠ΄ΠΈΠ»ΠΎ ΠΊ ΠΏΠΎΠ²Ρ‹ΡˆΠ΅Π½ΠΈΡŽ экспрСссии CD86 ΠΈ появлСнию экспрСссии ΠšΠ†67. ПослС Π°ΠΊΡ‚ΠΈΠ²Π°Ρ†ΠΈΠΈ M1 ΠΈ М2 ΠΈΠ½Π΄ΡƒΠΊΡ‚ΠΎΡ€Π°ΠΌΠΈ Ρƒ Ρ€Π΅Π·ΠΈΠ΄Π΅Π½Ρ‚Π½Ρ‹Ρ… ΠΌΠ°ΠΊΡ€ΠΎΡ„Π°Π³ΠΎΠ² ΠΏΠ΅Ρ‡Π΅Π½ΠΈ ΠΏΠΎΠ²Ρ‹ΡˆΠ°Π»Π°ΡΡŒ экспрСссия Π² основном ΠΏΡ€ΠΎΡ‚ΠΈΠ²ΠΎΠ²ΠΎΡΠΏΠ°Π»ΠΈΡ‚Π΅Π»ΡŒΠ½Ρ‹Ρ… Ρ†ΠΈΡ‚ΠΎΠΊΠΈΠ½ΠΎΠ². Π Π΅Π·ΠΈΠ΄Π΅Π½Ρ‚Π½Ρ‹Π΅ ΠΌΠ°ΠΊΡ€ΠΎΡ„Π°Π³ΠΈ ΠΏΠ΅Ρ‡Π΅Π½ΠΈ ΠΎΠ±Π»Π°Π΄Π°Π»ΠΈ Π²Ρ‹Ρ€Π°ΠΆΠ΅Π½Π½ΠΎΠΉ Ρ„Π°Π³ΠΎΡ†ΠΈΡ‚Π°Ρ€Π½ΠΎΠΉ Π°ΠΊΡ‚ΠΈΠ²Π½ΠΎΡΡ‚ΡŒΡŽ, которая сниТалась ΠΊ 5 часам, ΠΎΠ΄Π½Π°ΠΊΠΎ Π΄ΠΎΠ±Π°Π²Π»Π΅Π½ΠΈΠ΅ ΠΈΠ½Π΄ΡƒΠΊΡ‚ΠΎΡ€ΠΎΠ² ΠΊΠ°ΠΊ M1, Ρ‚Π°ΠΊ ΠΈ М2 Ρ„Π΅Π½ΠΎΡ‚ΠΈΠΏΠ° стимулировало Ρ„Π°Π³ΠΎΡ†ΠΈΡ‚ΠΎΠ· Π΄ΠΎ 24 часов послС внСсСния латСксных частиц

    ΠœΠžΠ Π€ΠžΠ€Π£ΠΠšΠ¦Π˜ΠžΠΠΠ›Π¬ΠΠΠ― Π₯ΠΠ ΠΠšΠ’Π•Π Π˜Π‘Π’Π˜ΠšΠ ΠœΠΠšΠ ΠžΠ€ΠΠ“ΠžΠ’ Π­ΠœΠ‘Π Π˜ΠžΠΠΠ›Π¬ΠΠžΠ“Πž И ΠœΠžΠΠžΠ¦Π˜Π’ΠΠ ΠΠžΠ“Πž ПРОИБΠ₯ΠžΠ–Π”Π•ΠΠ˜Π―

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    Macrophages of mammals are a heterogeneous population of cells. This applies both to the functional parameters of macrophages and to the sources of their development. The comparative characteristics of macrophages of embryonic origin on the example of Kupffer cells and macrophages of bone marrow origin on the example of macrophages of monocyte derivatives were carried out. Cultures of Kupffer cells and macrophages of monocytic origin were obtained. The phenotype, profile of gene expression of native macrophages activated in direction M1 and M2 was studied. The phenotype of isolated cultures is characterized by methods of immunocytochemistry, flow cytometry. Gene expression was studied by real-time polymerase chain reaction. Under the influence of inducing factors, the phenotype of two populations of macrophages changes in a similar way: under the influence of M1-factors, the synthesis of CD86 and iNOs is activated in cells, under the influence of M2 - CD163 and Arg1. In Kupffer cells, expression of anti-inflammatory cytokines - il4, il13, is more pronounced, and in macrophages of monocytic origin of pro-inflammatory cytokines - il1b, tnfa, il12a. The induction of the genes of proinflammatory cytokines in Kupffer cells is slower compared to macrophages of monocytic origin. Β© 2018 Human Stem Cell Institute. All rights reserved.ΠœΠ°ΠΊΡ€ΠΎΡ„Π°Π³ΠΈ ΠΌΠ»Π΅ΠΊΠΎΠΏΠΈΡ‚Π°ΡŽΡ‰ΠΈΡ… ΠΏΡ€Π΅Π΄ΡΡ‚Π°Π²Π»ΡΡŽΡ‚ собой Π³Π΅Ρ‚Π΅Ρ€ΠΎΠ³Π΅Π½Π½ΡƒΡŽ ΠΏΠΎΠΏΡƒΠ»ΡΡ†ΠΈΡŽ ΠΊΠ»Π΅Ρ‚ΠΎΠΊ, различия ΠΊΠ°ΡΠ°ΡŽΡ‚ΡΡ ΠΊΠ°ΠΊ источников ΠΈΡ… происхоТдСния, Ρ‚Π°ΠΊ ΠΈ Ρ„ΡƒΠ½ΠΊΡ†ΠΈΠΎΠ½Π°Π»ΡŒΠ½Ρ‹Ρ… ΠΏΠΎΠΊΠ°Π·Π°Ρ‚Π΅Π»Π΅ΠΉ. Π’ Ρ€Π°Π±ΠΎΡ‚Π΅ ΠΏΡ€ΠΎΠ²Π΅Π΄Π΅Π½Π° ΡΡ€Π°Π²Π½ΠΈΡ‚Π΅Π»ΡŒΠ½Π°Ρ характСристика ΠΌΠ°ΠΊΡ€ΠΎΡ„Π°Π³ΠΎΠ² ΡΠΌΠ±Ρ€ΠΈΠΎΠ½Π°Π»ΡŒΠ½ΠΎΠ³ΠΎ происхоТдСния Π½Π° ΠΏΡ€ΠΈΠΌΠ΅Ρ€Π΅ ΠΊΠ»Π΅Ρ‚ΠΎΠΊ ΠšΡƒΠΏΡ„Π΅Ρ€Π° ΠΈ ΠΌΠ°ΠΊΡ€ΠΎΡ„Π°Π³ΠΎΠ² костномозгового происхоТдСния Π½Π° ΠΏΡ€ΠΈΠΌΠ΅Ρ€Π΅ ΠΌΠ°ΠΊΡ€ΠΎΡ„Π°Π³ΠΎΠ² - ΠΏΡ€ΠΎΠΈΠ·Π²ΠΎΠ΄Π½Ρ‹Ρ… ΠΌΠΎΠ½ΠΎΡ†ΠΈΡ‚ΠΎΠ². Из ΠΏΠ΅Ρ‡Π΅Π½ΠΈ крыс Π±Ρ‹Π»Π° ΠΏΠΎΠ»ΡƒΡ‡Π΅Π½Π° ΠΊΡƒΠ»ΡŒΡ‚ΡƒΡ€Π° ΠΊΠ»Π΅Ρ‚ΠΎΠΊ ΠšΡƒΠΏΡ„Π΅Ρ€Π°, ΠΈΠ· ΠΊΡ€ΠΎΠ²ΠΈ крыс - ΠΌΠ°ΠΊΡ€ΠΎΡ„Π°Π³ΠΈ ΠΌΠΎΠ½ΠΎΡ†ΠΈΡ‚Π°Ρ€Π½ΠΎΠ³ΠΎ происхоТдСния. ΠžΡ†Π΅Π½ΠΈΠ²Π°Π»ΠΈ Ρ„Π΅Π½ΠΎΡ‚ΠΈΠΏ ΠΈ ΠΏΡ€ΠΎΡ„ΠΈΠ»ΡŒ экспрСссии Π³Π΅Π½ΠΎΠ² Π½Π°Ρ‚ΠΈΠ²Π½Ρ‹Ρ… ΠΈ Π°ΠΊΡ‚ΠΈΠ²ΠΈΡ€ΠΎΠ²Π°Π½Π½Ρ‹Ρ… Π² Π½Π°ΠΏΡ€Π°Π²Π»Π΅Π½ΠΈΠΈ М1- ΠΈ М2-ΠΌΠ°ΠΊΡ€ΠΎΡ„Π°Π³ΠΎΠ². Π€Π΅Π½ΠΎΡ‚ΠΈΠΏ Π²Ρ‹Π΄Π΅Π»Π΅Π½Π½Ρ‹Ρ… ΠΊΡƒΠ»ΡŒΡ‚ΡƒΡ€ Π±Ρ‹Π» ΠΎΡ…Π°Ρ€Π°ΠΊΡ‚Π΅Ρ€ΠΈΠ·ΠΎΠ²Π°Π½ с ΠΏΠΎΠΌΠΎΡ‰ΡŒΡŽ ΠΌΠ΅Ρ‚ΠΎΠ΄ΠΎΠ² ΠΈΠΌΠΌΡƒΠ½ΠΎΡ†ΠΈΡ‚ΠΎΡ…ΠΈΠΌΠΈΠΈ ΠΈ ΠΏΡ€ΠΎΡ‚ΠΎΡ‡Π½ΠΎΠΉ Ρ†ΠΈΡ‚ΠΎΡ„Π»ΡƒΠΎΡ€ΠΈΠΌΠ΅Ρ‚Ρ€ΠΈΠΈ. Π­ΠΊΡΠΏΡ€Π΅ΡΡΠΈΡŽ Π³Π΅Π½ΠΎΠ² ΠΈΠ·ΡƒΡ‡Π°Π»ΠΈ с ΠΏΠΎΠΌΠΎΡ‰ΡŒΡŽ ΠΏΠΎΠ»ΠΈΠΌΠ΅Ρ€Π°Π·Π½ΠΎΠΉ Ρ†Π΅ΠΏΠ½ΠΎΠΉ Ρ€Π΅Π°ΠΊΡ†ΠΈΠΈ Π² Ρ€Π΅Π°Π»ΡŒΠ½ΠΎΠΌ Π²Ρ€Π΅ΠΌΠ΅Π½ΠΈ. Под влияниСм ΠΈΠ½Π΄ΡƒΡ†ΠΈΡ€ΡƒΡŽΡ‰ΠΈΡ… Ρ„Π°ΠΊΡ‚ΠΎΡ€ΠΎΠ² Ρ„Π΅Π½ΠΎΡ‚ΠΈΠΏ Π΄Π²ΡƒΡ… популяций ΠΌΠ°ΠΊΡ€ΠΎΡ„Π°Π³ΠΎΠ² измСняСтся сходным ΠΎΠ±Ρ€Π°Π·ΠΎΠΌ: ΠΏΠΎΠ΄ влияниСм М1 Ρ„Π°ΠΊΡ‚ΠΎΡ€ΠΎΠ² Π² ΠΊΠ»Π΅Ρ‚ΠΊΠ°Ρ… активизируСтся синтСз CD86 ΠΈ iNOs, ΠΏΠΎΠ΄ влияниСм М2 - CD163 ΠΈ Arg1. Π’ ΠΊΠ»Π΅Ρ‚ΠΊΠ°Ρ… ΠšΡƒΠΏΡ„Π΅Ρ€Π° Π±ΠΎΠ»Π΅Π΅ Π²Ρ‹Ρ€Π°ΠΆΠ΅Π½Π° экспрСссия Π³Π΅Π½ΠΎΠ² ΠΏΡ€ΠΎΡ‚ΠΈΠ²ΠΎΠ²ΠΎΡΠΏΠ°Π»ΠΈΡ‚Π΅Π»ΡŒΠ½Ρ‹Ρ… Ρ†ΠΈΡ‚ΠΎΠΊΠΈΠ½ΠΎΠ² - il4, il13, Π° Π² ΠΌΠ°ΠΊΡ€ΠΎΡ„Π°Π³Π°Ρ… ΠΌΠΎΠ½ΠΎΡ†ΠΈΡ‚Π°Ρ€Π½ΠΎΠ³ΠΎ происхоТдСния экспрСссия Π³Π΅Π½ΠΎΠ² ΠΏΡ€ΠΎΠ²ΠΎΡΠΏΠ°Π»ΠΈΡ‚Π΅Π»ΡŒΠ½Ρ‹Ρ… Ρ†ΠΈΡ‚ΠΎΠΊΠΈΠ½ΠΎΠ² - il1b, tnfa, il12a. Π˜Π½Π΄ΡƒΠΊΡ†ΠΈΡ Π³Π΅Π½ΠΎΠ² ΠΏΡ€ΠΎΠ²ΠΎΡΠΏΠ°Π»ΠΈΡ‚Π΅Π»ΡŒΠ½Ρ‹Ρ… Ρ†ΠΈΡ‚ΠΎΠΊΠΈΠ½ΠΎΠ² Π² ΠΊΠ»Π΅Ρ‚ΠΊΠ°Ρ… ΠšΡƒΠΏΡ„Π΅Ρ€Π° происходит ΠΌΠ΅Π΄Π»Π΅Π½Π½Π΅Π΅ ΠΏΠΎ ΡΡ€Π°Π²Π½Π΅Π½ΠΈΡŽ с ΠΌΠ°ΠΊΡ€ΠΎΡ„Π°Π³Π°ΠΌΠΈ ΠΌΠΎΠ½ΠΎΡ†ΠΈΡ‚Π°Ρ€Π½ΠΎΠ³ΠΎ происхоТдСния

    ВлияниС ΡΠΊΡΠΏΠ΅Ρ€ΠΈΠΌΠ΅Π½Ρ‚Π°Π»ΡŒΠ½ΠΎΠ³ΠΎ модСлирования сСптопластики Π½Π° Ρ†ΠΈΡ‚ΠΎΠ°Ρ€Ρ…ΠΈΡ‚Π΅ΠΊΡ‚ΠΎΠ½ΠΈΠΊΡƒ Π³ΠΈΠΏΠΏΠΎΠΊΠ°ΠΌΠΏΠ° Ρƒ крыс

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    Objective: to evaluate the effect of septoplasty modeling on changes in the hippocampal cytoarchitectonics in rats. Material and methods. The study was conducted on 80 sexually mature male rats. In the experimental 1st and 2nd groups, premedication with phenazepamum solution was performed. The first group: 30 rats, local infiltration anesthesia with 2% lidocaine solution; the second group: 30 rats, local infiltration anesthesia with 2% ultracaine solution, postoperative analgesia with sodium diclofenac solution (6 days); the 3rd and 4th groups were control (10 animals each). In groups 1-3, pre-trepanation fixation of the brain was performed, in group 4 this was not done, and artifact dark neurons (DN) were counted. We studied the number of DN in the hippocampus on brain sections stained with hematoxylin-eosin on the 2nd, 6th and 14th days after surgery. Results. In the 2nd group, in the zones CA1, CA2, CA3 and DG, a smaller number of DN was observed compared with the 1st group on the 6th day (p <0.05), and on the 14th day in the 2nd group the number of DN was comparable with the 3rd group in zones CA1 and CA2 (p<0.05). In the 4th group, compared with the 3rd group, the number of DN was significantly higher in all hippocampal zones (p<0.05). Conclusions. Quantitative changes in DN may indicate the effect of surgical stress in the modeling of septoplasty and various anesthesia on changes in cytoarchitectonics in various parts of the hippocampus.ЦСль: ΠΎΡ†Π΅Π½ΠΈΡ‚ΡŒ влияниС модСлирования сСптопластики Π½Π° ΠΈΠ·ΠΌΠ΅Π½Π΅Π½ΠΈΠ΅ Ρ†ΠΈΡ‚ΠΎΠ°Ρ€Ρ…ΠΈΡ‚Π΅ΠΊΡ‚ΠΎΠ½ΠΈΠΊΠΈ Π³ΠΈΠΏΠΏΠΎΠΊΠ°ΠΌΠΏΠ° Ρƒ крыс. ΠœΠ°Ρ‚Π΅Ρ€ΠΈΠ°Π» ΠΈ ΠΌΠ΅Ρ‚ΠΎΠ΄Ρ‹. ИсслСдованиС ΠΏΡ€ΠΎΠ²ΠΎΠ΄ΠΈΠ»ΠΎΡΡŒ Π½Π° 80 ΠΏΠΎΠ»ΠΎΠ²ΠΎΠ·Ρ€Π΅Π»Ρ‹Ρ… крысах самцах. Π’ ΡΠΊΡΠΏΠ΅Ρ€ΠΈΠΌΠ΅Π½Ρ‚Π°Π»ΡŒΠ½Ρ‹Ρ… 1-ΠΉ ΠΈ 2-ΠΉ Π³Ρ€ΡƒΠΏΠΏΠ°Ρ… ΠΏΡ€ΠΎΠ²ΠΎΠ΄ΠΈΠ»Π°ΡΡŒ прСмСдикация раствором Ρ„Π΅Π½ΠΎΠ·Π΅ΠΏΠ°ΠΌΠ°. ΠŸΠ΅Ρ€Π²Π°Ρ Π³Ρ€ΡƒΠΏΠΏΠ°: 30 крыс, мСстная ΠΈΠ½Ρ„ΠΈΠ»ΡŒΡ‚Ρ€Π°Ρ†ΠΈΠΎΠ½Π½Π°Ρ анСстСзия 2% раствором Π»ΠΈΠ΄ΠΎΠΊΠ°ΠΈΠ½Π°; 2-я Π³Ρ€ΡƒΠΏΠΏΠ°: 30 крыс, мСстная ΠΈΠ½Ρ„ΠΈΠ»ΡŒΡ‚Ρ€Π°Ρ†ΠΈΠΎΠ½Π½Π°Ρ анСстСзия 2% раствором ΡƒΠ»ΡŒΡ‚Ρ€Π°ΠΊΠ°ΠΈΠ½Π°, послСопСрационная анальгСзия раствором Π΄ΠΈΠΊΠ»ΠΎΡ„Π΅Π½Π°ΠΊΠ° натрия (6 Π΄Π½Π΅ΠΉ); 3-я ΠΈ 4-я Π³Ρ€ΡƒΠΏΠΏΡ‹ Π±Ρ‹Π»ΠΈ ΠΊΠΎΠ½Ρ‚Ρ€ΠΎΠ»ΡŒΠ½Ρ‹ΠΌΠΈ (ΠΏΠΎ 10 ΠΆΠΈΠ²ΠΎΡ‚Π½Ρ‹Ρ…). Π’ 1-3-ΠΉ Π³Ρ€ΡƒΠΏΠΏΠ°Ρ… ΠΏΡ€ΠΎΠ²ΠΎΠ΄ΠΈΠ»Π°ΡΡŒ прСдтрСпанационная фиксация Π³ΠΎΠ»ΠΎΠ²Π½ΠΎΠ³ΠΎ ΠΌΠΎΠ·Π³Π°, Π² 4-ΠΉ Π³Ρ€ΡƒΠΏΠΏΠ΅ это Π½Π΅ ΠΏΡ€ΠΎΠ²ΠΎΠ΄ΠΈΠ»ΠΈ, Π° подсчитывали Π°Ρ€Ρ‚Π΅Ρ„Π°ΠΊΡ‚Π½Ρ‹Π΅ Ρ‚Π΅ΠΌΠ½Ρ‹Π΅ Π½Π΅ΠΉΡ€ΠΎΠ½Ρ‹ (ВН). Π˜Π·ΡƒΡ‡Π°Π»ΠΈ число ВН Π² Π³ΠΈΠΏΠΏΠΎΠΊΠ°ΠΌΠΏΠ΅ Π½Π° срСзах Π³ΠΎΠ»ΠΎΠ²Π½ΠΎΠ³ΠΎ ΠΌΠΎΠ·Π³Π°, ΠΎΠΊΡ€Π°ΡˆΠ΅Π½Π½Ρ‹Ρ… гСматоксилин-эозином, Π½Π° 2-ΠΉ, 6 ΠΈ 14-ΠΉ Π΄Π½ΠΈ послС ΠΎΠΏΠ΅Ρ€Π°Ρ†ΠΈΠΈ. Π Π΅Π·ΡƒΠ»ΡŒΡ‚Π°Ρ‚Ρ‹. Π’ΠΎ 2-ΠΉ Π³Ρ€ΡƒΠΏΠΏΠ΅ Π² Π·ΠΎΠ½Π°Ρ… БА1, БА2, БА3 ΠΈ DG наблюдалось мСньшСС число ВН ΠΏΠΎ ΡΡ€Π°Π²Π½Π΅Π½ΠΈΡŽ с 1-ΠΉ Π³Ρ€ΡƒΠΏΠΏΠΎΠΉ Π½Π° 6-ΠΉ дСнь (p<0,05), Π° Π½Π° 14-ΠΉ дСнь Π²ΠΎ 2-ΠΉ Π³Ρ€ΡƒΠΏΠΏΠ΅ число ВН Π±Ρ‹Π»ΠΎ сопоставимо с 3-ΠΉ Π³Ρ€ΡƒΠΏΠΏΠΎΠΉ Π² Π·ΠΎΠ½Π°Ρ… БА1 ΠΈ БА2 (p<0,05). Π’ 4-ΠΉ Π³Ρ€ΡƒΠΏΠΏΠ΅ ΠΏΠΎ ΡΡ€Π°Π²Π½Π΅Π½ΠΈΡŽ с 3-ΠΉ Π³Ρ€ΡƒΠΏΠΏΠΎΠΉ число ВН Π±Ρ‹Π»ΠΎ достовСрно Π²Ρ‹ΡˆΠ΅ Π²ΠΎ всСх Π³ΠΈΠΏΠΏΠΎΠΊΠ°ΠΌΠΏΠ°Π»ΡŒΠ½Ρ‹Ρ… Π·ΠΎΠ½Π°Ρ… (p<0,05). Π’Ρ‹Π²ΠΎΠ΄Ρ‹. ΠšΠΎΠ»ΠΈΡ‡Π΅ΡΡ‚Π²Π΅Π½Π½Ρ‹Π΅ измСнСния ВН ΠΌΠΎΠ³ΡƒΡ‚ ΡΠ²ΠΈΠ΄Π΅Ρ‚Π΅Π»ΡŒΡΡ‚Π²ΠΎΠ²Π°Ρ‚ΡŒ ΠΎ влиянии хирургичСского стрСсса ΠΏΡ€ΠΈ ΠΌΠΎΠ΄Π΅Π»ΠΈΡ€ΠΎΠ²Π°Π½ΠΈΠΈ сСптопластики ΠΈ Ρ€Π°Π·Π»ΠΈΡ‡Π½ΠΎΠΌ анСстСзилогичСском пособии Π½Π° измСнСния Ρ†ΠΈΡ‚ΠΎΠ°Ρ€Ρ…ΠΈΡ‚Π΅ΠΊΡ‚ΠΎΠ½ΠΈΠΊΠΈ Π² Ρ€Π°Π·Π»ΠΈΡ‡Π½Ρ‹Ρ… ΠΎΡ‚Π΄Π΅Π»Π°Ρ… Π³ΠΈΠΏΠΏΠΎΠΊΠ°ΠΌΠΏΠ°

    Π˜Π—Π£Π§Π•ΠΠ˜Π• Π’Π›Π˜Π―ΠΠ˜Π― БВРЕББА НА ΠœΠžΠ Π€ΠžΠ›ΠžΠ“Π˜Π§Π•Π‘ΠšΠ˜Π• Π˜Π—ΠœΠ•ΠΠ•ΠΠ˜Π― Π’ Π“Π˜ΠŸΠŸΠžΠšΠΠœΠŸΠ• ПРИ Π₯Π˜Π Π£Π Π“Π˜Π§Π•Π‘ΠšΠ˜Π₯ Π’ΠœΠ•Π¨ΠΠ’Π•Π›Π¬Π‘Π’Π’ΠΠ₯ Π’ ΠžΠ‘Π›ΠΠ‘Π’Π˜ НОБА

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    Objective - to study the morphological changes in the hippocampus of the rat brain following modeling septoplasty. Material and methods. The study was conducted on 80 adult male rats. In the experimental groups1 and 2, premedica tion with Phenazepamum was carried out. Group 1 included 30 rats subjected to local infiltration anesthesia with 2 % lidocaine solution; group 2 included 30 rats subjected to local infiltration anesthesia with 2 % solution of articain & epinephrine (0,5 mg/100 ml) and postoperative analgesia with diclofenac sodium solution for 6 days after surgery. Groups 3 and 4 were controls and included 10 animals each. In groups 1-3, fixation of the brain was performed prior to trepanation; in group 4, similar fixation was not performed and artifact dark neurons were counted. The number of dark neurons in the hippocampus was counted on hematoxylin-eosin stained brain sections on days 2, 6 and 14 after surgery. Results. In group 2, in the CA1, CA2, CA3 and DG zones, there were fewer dark neurons (DN) on day 6 compared with group 1. On 14th day, the number of DN in areas CA1 and CA2 was comparable in groups 2 and 3. In group 4, compared with group 3, the number of DN was greater in all studied hippocampal zones. Conclusion. The quantitative changes of DN in different zones of the hippocampus may indicate the severity of surgical stress following simulation of septoplasty under different types of anesthesia.ЦСль - ΠΈΠ·ΡƒΡ‡ΠΈΡ‚ΡŒ морфологичСскиС измСнСния Π² Π³ΠΈΠΏΠΏΠΎΠΊΠ°ΠΌΠΏΠ΅ ΠΌΠΎΠ·Π³Π° крыс ΠΏΡ€ΠΈ ΠΌΠΎΠ΄Π΅Π»ΠΈΡ€ΠΎΠ²Π°Π½ΠΈΠΈ пластики носовой ΠΏΠ΅Ρ€Π΅Π³ΠΎΡ€ΠΎΠ΄ΠΊΠΈ. ΠœΠ°Ρ‚Π΅Ρ€ΠΈΠ°Π»Ρ‹ ΠΈ ΠΌΠ΅Ρ‚ΠΎΠ΄Ρ‹. Π Π°Π±ΠΎΡ‚Π° Π²Ρ‹ΠΏΠΎΠ»Π½Π΅Π½Π° Π½Π° 80 ΠΏΠΎΠ»ΠΎΠ²ΠΎΠ·Ρ€Π΅Π»Ρ‹Ρ… крысах-самцах. Π’ ΡΠΊΡΠΏΠ΅Ρ€ΠΈΠΌΠ΅Π½Ρ‚Π°Π»ΡŒΠ½Ρ‹Ρ… 1-ΠΉΠΈ 2-ΠΉ Π³Ρ€ΡƒΠΏΠΏΠ΅ ΠΏΡ€ΠΎΠ²ΠΎΠ΄ΠΈΠ»ΠΈ ΠΏΡ€Π΅ΠΌΠ΅Π΄ΠΈΠΊΠ°Ρ†ΠΈΡŽ раствором Ρ„Π΅Π½ΠΎΠ·Π΅ΠΏΠ°ΠΌΠ°. 1-я Π³Ρ€ΡƒΠΏΠΏΠ° Π²ΠΊΠ»ΡŽΡ‡Π°Π»Π° 30 крыс, ΠΏΠΎΠ΄Π²Π΅Ρ€Π³Π½ΡƒΡ‚Ρ‹Ρ… мСстной ΠΈΠ½Ρ„ΠΈΠ»ΡŒΡ‚Ρ€Π°Ρ†ΠΈΠΎΠ½Π½ΠΎΠΉ анСстСзии 2 % раствором Π»ΠΈΠ΄ΠΎΠΊΠ°ΠΈΠ½Π°; 2-я Π³Ρ€ΡƒΠΏΠΏΠ° - 30 крыс, ΠΏΠΎΠ΄Π²Π΅Ρ€Π³Π½ΡƒΡ‚Ρ‹Ρ… мСстной ΠΈΠ½Ρ„ΠΈΠ»ΡŒΡ‚Ρ€Π°Ρ†ΠΈΠΎΠ½Π½ΠΎΠΉ анСстСзии 2 % раствором Π°Ρ€Ρ‚ΠΈΠΊΠ°ΠΈΠ½Π° ΠΈ эпинСфрина (0,5 ΠΌΠ³/100 ΠΌΠ»), послСопСрационной Π°Π½Π°Π»Π³Π΅Π·ΠΈΠΈ раствором Π΄ΠΈΠΊΠ»ΠΎΡ„Π΅Π½Π°ΠΊΠ° натрия Π² Ρ‚Π΅Ρ‡Π΅Π½ΠΈΠ΅ 6 сут послС ΠΎΠΏΠ΅Ρ€Π°Ρ†ΠΈΠΈ. 3-яи 4-я Π³Ρ€ΡƒΠΏΠΏΠ° Π±Ρ‹Π»ΠΈ ΠΊΠΎΠ½Ρ‚Ρ€ΠΎΠ»ΡŒΠ½Ρ‹ΠΌΠΈ ΠΈ Π²ΠΊΠ»ΡŽΡ‡Π°Π»ΠΈ ΠΏΠΎ 10 ΠΆΠΈΠ²ΠΎΡ‚Π½Ρ‹Ρ…. Π’ 1-3-ΠΉ Π³Ρ€ΡƒΠΏΠΏΠ΅ ΠΏΡ€ΠΎΠ²ΠΎΠ΄ΠΈΠ»Π°ΡΡŒ прСдтрСпанационная фиксация Π³ΠΎΠ»ΠΎΠ²Π½ΠΎΠ³ΠΎ ΠΌΠΎΠ·Π³Π°, Π² 4-ΠΉ Π³Ρ€ΡƒΠΏΠΏΠ΅ ΠΏΠΎΠ΄ΠΎΠ±Π½ΠΎΠΉ фиксации Π½Π΅ ΠΏΡ€ΠΎΠ²ΠΎΠ΄ΠΈΠ»ΠΎΡΡŒ ΠΈ ΠΏΠΎΠ΄ΡΡ‡ΠΈΡ‚Ρ‹Π²Π°Π»ΠΈΡΡŒ Π°Ρ€Ρ‚Π΅Ρ„Π°ΠΊΡ‚Π½Ρ‹Π΅ Ρ‚Π΅ΠΌΠ½Ρ‹Π΅ Π½Π΅ΠΉΡ€ΠΎΠ½Ρ‹ (ВН). Π˜Π·ΡƒΡ‡Π°Π»ΠΈ число ВН Π² Π³ΠΈΠΏΠΏΠΎΠΊΠ°ΠΌΠΏΠ΅ Π½Π° срСзах Π³ΠΎΠ»ΠΎΠ²Π½ΠΎΠ³ΠΎ ΠΌΠΎΠ·Π³Π°, ΠΎΠΊΡ€Π°ΡˆΠ΅Π½Π½Ρ‹Ρ… гСматоксилином ΠΈ эозином, Π½Π° 2-, 6-Π΅ΠΈ 14-Π΅ сутки послС ΠΎΠΏΠ΅Ρ€Π°Ρ†ΠΈΠΈ. Π Π΅Π·ΡƒΠ»ΡŒΡ‚Π°Ρ‚Ρ‹. Π’ΠΎ 2-ΠΉ Π³Ρ€ΡƒΠΏΠΏΠ΅ Π² Π·ΠΎΠ½Π°Ρ… БА1, БА2, БА3 ΠΈ DG наблюдали мСньшСС число ВН ΠΏΠΎ ΡΡ€Π°Π²Π½Π΅Π½ΠΈΡŽ с 1-ΠΉ Π³Ρ€ΡƒΠΏΠΏΠΎΠΉ Π½Π° 6-Π΅ сутки. На 14-Π΅ сутки число ВН Π²ΠΎ 2-ΠΉΠΈ 3-ΠΉ Π³Ρ€ΡƒΠΏΠΏΠ΅ Π±Ρ‹Π»ΠΎ сопоставимо Π² Π·ΠΎΠ½Π°Ρ… БА1 ΠΈ БА2. Π’ 4-ΠΉ Π³Ρ€ΡƒΠΏΠΏΠ΅ ΠΏΠΎ ΡΡ€Π°Π²Π½Π΅Π½ΠΈΡŽ с 3-ΠΉ Π³Ρ€ΡƒΠΏΠΏΠΎΠΉ число ВН Π±Ρ‹Π»ΠΎ большС Π²ΠΎ всСх исслСдованных Π·ΠΎΠ½Π°Ρ… Π³ΠΈΠΏΠΏΠΎΠΊΠ°ΠΌΠΏΠ°. Π’Ρ‹Π²ΠΎΠ΄. ΠšΠΎΠ»ΠΈΡ‡Π΅ΡΡ‚Π²Π΅Π½Π½Ρ‹Π΅ измСнСния ВН Π² Ρ€Π°Π·Π»ΠΈΡ‡Π½Ρ‹Ρ… Π·ΠΎΠ½Π°Ρ… Π³ΠΈΠΏΠΏΠΎΠΊΠ°ΠΌΠΏΠ° ΠΌΠΎΠ³ΡƒΡ‚ ΡΠ²ΠΈΠ΄Π΅Ρ‚Π΅Π»ΡŒΡΡ‚Π²ΠΎΠ²Π°Ρ‚ΡŒ ΠΎ Π²Ρ‹Ρ€Π°ΠΆΠ΅Π½Π½ΠΎΠΌ хирургичСском стрСссС Π²ΠΎ врСмя модСлирования сСптопластики ΠΏΡ€ΠΈ Ρ€Π°Π·Π»ΠΈΡ‡Π½ΠΎΠΌ анСстСзиологичСском пособии
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