22 research outputs found
EPR spectroscopy of <sup>53</sup>Cr monoisotopic impurity ions in a single crystal of yttrium orthosilicate Y<inf>2</inf>SiO<inf>5</inf>
Β© 2020 Elsevier B.V. The electron paramagnetic resonance spectra of 53Cr monoisotopic impurity ions in yttrium orthosilicate single crystals (Y2SiO5) were studied by electron paramagnetic resonance (EPR) in the X-band. The directions of the principal magnetic axes and the parameters of the effective spin Hamiltonian, which describes magnetic characteristics of the impurity centers of chromium are determined. It is shown that the orientation dependencies of the EPR spectra are described by the second-order spin Hamiltonian corresponding to the rhombic symmetry of the local crystal field acting on the impurity ion. It was assumed that the g-tensor and A-tensor describing the Zeeman energy of electron levels in a magnetic field and the hyperfine interaction of electron and nuclear spins are isotropic, and the entire anisotropy of the EPR spectra is due to the anisotropy of the D-tensor, which describes the fine structure of electron levels in crystal electric field. A strong dependence of the probability of forbidden transitions between hyperfine sublevels of electron levels on the orientation of an external magnetic field is established. Moreover, for some orientations, the probability of forbidden transitions becomes comparable to the probability of allowed transitions
Iron oxidation state in La<inf>0.7</inf>Sr<inf>1.3</inf>Fe<inf>0.7</inf>Ti<inf>0.3</inf>O<inf>4</inf> and La<inf>0.5</inf>Sr<inf>1.5</inf>Fe<inf>0.5</inf>Ti<inf>0.5</inf>O<inf>4</inf> layered perovskites: Magnetic properties
La0.5Sr1.5Fe0.5Ti0.5O4 and La0.7Sr1.3Fe0.7Ti0.3O4 solid solutions with the layered perovskite structure were synthesized using a solid state method. Structural properties of obtained samples were characterized using X-ray diffraction and X-ray fluorescence analyses. Magnetic properties were investigated using magnetometry, electron spin resonance (ESR) and MΓΆssbauer spectroscopy methods. Based on magnetization and ESR measurements it was suggested the presence of Fe4+ ions in addition to trivalent iron ions that was exactly confirmed by MΓΆssbauer spectroscopy investigations. Based on all experimental results one can suggest the presence of the electronic phase separation in the investigated samples β the simultaneous existence of the paramagnetic phase and magnetically correlated regions, which form due to the mixed-valence iron ions. So the paramagnetic phase with strong antiferromagnetic correlation exists in both samples, while the second phase is ferromagnetically and ferrimagnetically correlated regions in La0.5Sr1.5Fe0.5Ti0.5O4 and La0.7Sr1.3Fe0.7Ti0.3O4, respectively
Comparative study of the biocompatibility of polydioxanone and polyglycolide absorbable meshes for hernia and pelvic defects repair
Π₯Π°ΡΠ°ΠΊΡΠ΅ΡΠΈΡΡΠΈΠΊΠ° ΠΈΠΌΠΌΡΠ½ΠΎΡΠ΅Π½ΠΎΡΠΈΠΏΠ° ΡΠ΅Π·ΠΈΠ΄Π΅Π½ΡΠ½ΡΡ ΠΌΠ°ΠΊΡΠΎΡΠ°Π³ΠΎΠ² ΠΏΠ΅ΡΠ΅Π½ΠΈ ΠΈ ΠΏΡΠΎΡΠΈΠ»Ρ ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΡΡΠ΅ΠΌΡΡ Π³Π΅Π½ΠΎΠ²
The profile of the expressed genes, phenotype and phagocytic activity of resident macrophages of the liver were studied. It was found that most of the resident liver macrophages were F4/80+, CD68+, CD163+, and CD11b-. Induction of Π2 factor phenotypes increased CD86 expression as well as triggered ΠΠ67 expression. After activation of Ml and Π2 by inducers, resident liver macrophages enhanced the expression of main anti-inflammatory cytokines. Resident liver macrophages showed pronounced phagocytic activity, which faded up to 5 hours after the administration of latex particles, but the addition of inducers of both the Ml and Π2 phenotype stimulated phagocytosis during 24 hours.ΠΠ·ΡΡΠ΅Π½Ρ ΠΏΡΠΎΡΠΈΠ»Ρ ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΡΡΠ΅ΠΌΡΡ
Π³Π΅Π½ΠΎΠ² ΡΠΈΡΠΎΠΊΠΈΠ½ΠΎΠ² ΠΈ ΠΌΠ°ΡΠΊΠ΅ΡΠΎΠ² ΠΌΠ°ΠΊΡΠΎΡΠ°Π³ΠΎΠ², ΡΠ΅Π½ΠΎΡΠΈΠΏ ΠΈ ΡΠ°Π³ΠΎΡΠΈΡΠ°ΡΠ½Π°Ρ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΡ ΡΠ΅Π·ΠΈΠ΄Π΅Π½ΡΠ½ΡΡ
ΠΌΠ°ΠΊΡΠΎΡΠ°Π³ΠΎΠ² ΠΏΠ΅ΡΠ΅Π½ΠΈ. ΠΠ±Π½Π°ΡΡΠΆΠ΅Π½ΠΎ, ΡΡΠΎ Π±ΠΎΠ»ΡΡΠ°Ρ ΡΠ°ΡΡΡ ΡΠ΅Π·ΠΈΠ΄Π΅Π½ΡΠ½ΡΡ
ΠΌΠ°ΠΊΡΠΎΡΠ°Π³ΠΎΠ² ΠΏΠ΅ΡΠ΅Π½ΠΈ ΠΈΠΌΠ΅Π»Π° ΡΠ΅Π½ΠΎΡΠΈΠΏ F4/80+, CD68+, CD163+, CD lib". ΠΠΎΠ·Π΄Π΅ΠΉΡΡΠ²ΠΈΠ΅ ΡΠ°ΠΊΡΠΎΡΠΎΠ², ΠΏΠΎΠ»ΡΡΠΈΠ·ΡΡΡΠΈΡ
ΠΌΠ°ΠΊΡΠΎΡΠ°Π³ΠΈ Π² Π½Π°ΠΏΡΠ°Π²Π»Π΅Π½ΠΈΠΈ Π2-ΡΠ΅Π½ΠΎΡΠΈΠΏΠ°, ΠΏΡΠΈΠ²ΠΎΠ΄ΠΈΠ»ΠΎ ΠΊ ΠΏΠΎΠ²ΡΡΠ΅Π½ΠΈΡ ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΠΈ CD86 ΠΈ ΠΏΠΎΡΠ²Π»Π΅Π½ΠΈΡ ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΠΈ ΠΠ67. ΠΠΎΡΠ»Π΅ Π°ΠΊΡΠΈΠ²Π°ΡΠΈΠΈ M1 ΠΈ Π2 ΠΈΠ½Π΄ΡΠΊΡΠΎΡΠ°ΠΌΠΈ Ρ ΡΠ΅Π·ΠΈΠ΄Π΅Π½ΡΠ½ΡΡ
ΠΌΠ°ΠΊΡΠΎΡΠ°Π³ΠΎΠ² ΠΏΠ΅ΡΠ΅Π½ΠΈ ΠΏΠΎΠ²ΡΡΠ°Π»Π°ΡΡ ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΡ Π² ΠΎΡΠ½ΠΎΠ²Π½ΠΎΠΌ ΠΏΡΠΎΡΠΈΠ²ΠΎΠ²ΠΎΡΠΏΠ°Π»ΠΈΡΠ΅Π»ΡΠ½ΡΡ
ΡΠΈΡΠΎΠΊΠΈΠ½ΠΎΠ². Π Π΅Π·ΠΈΠ΄Π΅Π½ΡΠ½ΡΠ΅ ΠΌΠ°ΠΊΡΠΎΡΠ°Π³ΠΈ ΠΏΠ΅ΡΠ΅Π½ΠΈ ΠΎΠ±Π»Π°Π΄Π°Π»ΠΈ Π²ΡΡΠ°ΠΆΠ΅Π½Π½ΠΎΠΉ ΡΠ°Π³ΠΎΡΠΈΡΠ°ΡΠ½ΠΎΠΉ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΡΡ, ΠΊΠΎΡΠΎΡΠ°Ρ ΡΠ½ΠΈΠΆΠ°Π»Π°ΡΡ ΠΊ 5 ΡΠ°ΡΠ°ΠΌ, ΠΎΠ΄Π½Π°ΠΊΠΎ Π΄ΠΎΠ±Π°Π²Π»Π΅Π½ΠΈΠ΅ ΠΈΠ½Π΄ΡΠΊΡΠΎΡΠΎΠ² ΠΊΠ°ΠΊ M1, ΡΠ°ΠΊ ΠΈ Π2 ΡΠ΅Π½ΠΎΡΠΈΠΏΠ° ΡΡΠΈΠΌΡΠ»ΠΈΡΠΎΠ²Π°Π»ΠΎ ΡΠ°Π³ΠΎΡΠΈΡΠΎΠ· Π΄ΠΎ 24 ΡΠ°ΡΠΎΠ² ΠΏΠΎΡΠ»Π΅ Π²Π½Π΅ΡΠ΅Π½ΠΈΡ Π»Π°ΡΠ΅ΠΊΡΠ½ΡΡ
ΡΠ°ΡΡΠΈΡ
Characteristics of the phagocytic activity of macrophages of bone marrow and fetal origin
ΠΠΠ Π€ΠΠ€Π£ΠΠΠ¦ΠΠΠΠΠΠ¬ΠΠΠ― Π₯ΠΠ ΠΠΠ’ΠΠ ΠΠ‘Π’ΠΠΠ ΠΠΠΠ ΠΠ€ΠΠΠΠ ΠΠΠΠ ΠΠΠΠΠΠ¬ΠΠΠΠ Π ΠΠΠΠΠ¦ΠΠ’ΠΠ ΠΠΠΠ ΠΠ ΠΠΠ‘Π₯ΠΠΠΠΠΠΠ―
Macrophages of mammals are a heterogeneous population of cells. This applies both to the functional parameters of macrophages and to the sources of their development. The comparative characteristics of macrophages of embryonic origin on the example of Kupffer cells and macrophages of bone marrow origin on the example of macrophages of monocyte derivatives were carried out. Cultures of Kupffer cells and macrophages of monocytic origin were obtained. The phenotype, profile of gene expression of native macrophages activated in direction M1 and M2 was studied. The phenotype of isolated cultures is characterized by methods of immunocytochemistry, flow cytometry. Gene expression was studied by real-time polymerase chain reaction. Under the influence of inducing factors, the phenotype of two populations of macrophages changes in a similar way: under the influence of M1-factors, the synthesis of CD86 and iNOs is activated in cells, under the influence of M2 - CD163 and Arg1. In Kupffer cells, expression of anti-inflammatory cytokines - il4, il13, is more pronounced, and in macrophages of monocytic origin of pro-inflammatory cytokines - il1b, tnfa, il12a. The induction of the genes of proinflammatory cytokines in Kupffer cells is slower compared to macrophages of monocytic origin. Β© 2018 Human Stem Cell Institute. All rights reserved.ΠΠ°ΠΊΡΠΎΡΠ°Π³ΠΈ ΠΌΠ»Π΅ΠΊΠΎΠΏΠΈΡΠ°ΡΡΠΈΡ
ΠΏΡΠ΅Π΄ΡΡΠ°Π²Π»ΡΡΡ ΡΠΎΠ±ΠΎΠΉ Π³Π΅ΡΠ΅ΡΠΎΠ³Π΅Π½Π½ΡΡ ΠΏΠΎΠΏΡΠ»ΡΡΠΈΡ ΠΊΠ»Π΅ΡΠΎΠΊ, ΡΠ°Π·Π»ΠΈΡΠΈΡ ΠΊΠ°ΡΠ°ΡΡΡΡ ΠΊΠ°ΠΊ ΠΈΡΡΠΎΡΠ½ΠΈΠΊΠΎΠ² ΠΈΡ
ΠΏΡΠΎΠΈΡΡ
ΠΎΠΆΠ΄Π΅Π½ΠΈΡ, ΡΠ°ΠΊ ΠΈ ΡΡΠ½ΠΊΡΠΈΠΎΠ½Π°Π»ΡΠ½ΡΡ
ΠΏΠΎΠΊΠ°Π·Π°ΡΠ΅Π»Π΅ΠΉ. Π ΡΠ°Π±ΠΎΡΠ΅ ΠΏΡΠΎΠ²Π΅Π΄Π΅Π½Π° ΡΡΠ°Π²Π½ΠΈΡΠ΅Π»ΡΠ½Π°Ρ Ρ
Π°ΡΠ°ΠΊΡΠ΅ΡΠΈΡΡΠΈΠΊΠ° ΠΌΠ°ΠΊΡΠΎΡΠ°Π³ΠΎΠ² ΡΠΌΠ±ΡΠΈΠΎΠ½Π°Π»ΡΠ½ΠΎΠ³ΠΎ ΠΏΡΠΎΠΈΡΡ
ΠΎΠΆΠ΄Π΅Π½ΠΈΡ Π½Π° ΠΏΡΠΈΠΌΠ΅ΡΠ΅ ΠΊΠ»Π΅ΡΠΎΠΊ ΠΡΠΏΡΠ΅ΡΠ° ΠΈ ΠΌΠ°ΠΊΡΠΎΡΠ°Π³ΠΎΠ² ΠΊΠΎΡΡΠ½ΠΎΠΌΠΎΠ·Π³ΠΎΠ²ΠΎΠ³ΠΎ ΠΏΡΠΎΠΈΡΡ
ΠΎΠΆΠ΄Π΅Π½ΠΈΡ Π½Π° ΠΏΡΠΈΠΌΠ΅ΡΠ΅ ΠΌΠ°ΠΊΡΠΎΡΠ°Π³ΠΎΠ² - ΠΏΡΠΎΠΈΠ·Π²ΠΎΠ΄Π½ΡΡ
ΠΌΠΎΠ½ΠΎΡΠΈΡΠΎΠ². ΠΠ· ΠΏΠ΅ΡΠ΅Π½ΠΈ ΠΊΡΡΡ Π±ΡΠ»Π° ΠΏΠΎΠ»ΡΡΠ΅Π½Π° ΠΊΡΠ»ΡΡΡΡΠ° ΠΊΠ»Π΅ΡΠΎΠΊ ΠΡΠΏΡΠ΅ΡΠ°, ΠΈΠ· ΠΊΡΠΎΠ²ΠΈ ΠΊΡΡΡ - ΠΌΠ°ΠΊΡΠΎΡΠ°Π³ΠΈ ΠΌΠΎΠ½ΠΎΡΠΈΡΠ°ΡΠ½ΠΎΠ³ΠΎ ΠΏΡΠΎΠΈΡΡ
ΠΎΠΆΠ΄Π΅Π½ΠΈΡ. ΠΡΠ΅Π½ΠΈΠ²Π°Π»ΠΈ ΡΠ΅Π½ΠΎΡΠΈΠΏ ΠΈ ΠΏΡΠΎΡΠΈΠ»Ρ ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΠΈ Π³Π΅Π½ΠΎΠ² Π½Π°ΡΠΈΠ²Π½ΡΡ
ΠΈ Π°ΠΊΡΠΈΠ²ΠΈΡΠΎΠ²Π°Π½Π½ΡΡ
Π² Π½Π°ΠΏΡΠ°Π²Π»Π΅Π½ΠΈΠΈ Π1- ΠΈ Π2-ΠΌΠ°ΠΊΡΠΎΡΠ°Π³ΠΎΠ². Π€Π΅Π½ΠΎΡΠΈΠΏ Π²ΡΠ΄Π΅Π»Π΅Π½Π½ΡΡ
ΠΊΡΠ»ΡΡΡΡ Π±ΡΠ» ΠΎΡ
Π°ΡΠ°ΠΊΡΠ΅ΡΠΈΠ·ΠΎΠ²Π°Π½ Ρ ΠΏΠΎΠΌΠΎΡΡΡ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠ² ΠΈΠΌΠΌΡΠ½ΠΎΡΠΈΡΠΎΡ
ΠΈΠΌΠΈΠΈ ΠΈ ΠΏΡΠΎΡΠΎΡΠ½ΠΎΠΉ ΡΠΈΡΠΎΡΠ»ΡΠΎΡΠΈΠΌΠ΅ΡΡΠΈΠΈ. ΠΠΊΡΠΏΡΠ΅ΡΡΠΈΡ Π³Π΅Π½ΠΎΠ² ΠΈΠ·ΡΡΠ°Π»ΠΈ Ρ ΠΏΠΎΠΌΠΎΡΡΡ ΠΏΠΎΠ»ΠΈΠΌΠ΅ΡΠ°Π·Π½ΠΎΠΉ ΡΠ΅ΠΏΠ½ΠΎΠΉ ΡΠ΅Π°ΠΊΡΠΈΠΈ Π² ΡΠ΅Π°Π»ΡΠ½ΠΎΠΌ Π²ΡΠ΅ΠΌΠ΅Π½ΠΈ. ΠΠΎΠ΄ Π²Π»ΠΈΡΠ½ΠΈΠ΅ΠΌ ΠΈΠ½Π΄ΡΡΠΈΡΡΡΡΠΈΡ
ΡΠ°ΠΊΡΠΎΡΠΎΠ² ΡΠ΅Π½ΠΎΡΠΈΠΏ Π΄Π²ΡΡ
ΠΏΠΎΠΏΡΠ»ΡΡΠΈΠΉ ΠΌΠ°ΠΊΡΠΎΡΠ°Π³ΠΎΠ² ΠΈΠ·ΠΌΠ΅Π½ΡΠ΅ΡΡΡ ΡΡ
ΠΎΠ΄Π½ΡΠΌ ΠΎΠ±ΡΠ°Π·ΠΎΠΌ: ΠΏΠΎΠ΄ Π²Π»ΠΈΡΠ½ΠΈΠ΅ΠΌ Π1 ΡΠ°ΠΊΡΠΎΡΠΎΠ² Π² ΠΊΠ»Π΅ΡΠΊΠ°Ρ
Π°ΠΊΡΠΈΠ²ΠΈΠ·ΠΈΡΡΠ΅ΡΡΡ ΡΠΈΠ½ΡΠ΅Π· CD86 ΠΈ iNOs, ΠΏΠΎΠ΄ Π²Π»ΠΈΡΠ½ΠΈΠ΅ΠΌ Π2 - CD163 ΠΈ Arg1. Π ΠΊΠ»Π΅ΡΠΊΠ°Ρ
ΠΡΠΏΡΠ΅ΡΠ° Π±ΠΎΠ»Π΅Π΅ Π²ΡΡΠ°ΠΆΠ΅Π½Π° ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΡ Π³Π΅Π½ΠΎΠ² ΠΏΡΠΎΡΠΈΠ²ΠΎΠ²ΠΎΡΠΏΠ°Π»ΠΈΡΠ΅Π»ΡΠ½ΡΡ
ΡΠΈΡΠΎΠΊΠΈΠ½ΠΎΠ² - il4, il13, Π° Π² ΠΌΠ°ΠΊΡΠΎΡΠ°Π³Π°Ρ
ΠΌΠΎΠ½ΠΎΡΠΈΡΠ°ΡΠ½ΠΎΠ³ΠΎ ΠΏΡΠΎΠΈΡΡ
ΠΎΠΆΠ΄Π΅Π½ΠΈΡ ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΡ Π³Π΅Π½ΠΎΠ² ΠΏΡΠΎΠ²ΠΎΡΠΏΠ°Π»ΠΈΡΠ΅Π»ΡΠ½ΡΡ
ΡΠΈΡΠΎΠΊΠΈΠ½ΠΎΠ² - il1b, tnfa, il12a. ΠΠ½Π΄ΡΠΊΡΠΈΡ Π³Π΅Π½ΠΎΠ² ΠΏΡΠΎΠ²ΠΎΡΠΏΠ°Π»ΠΈΡΠ΅Π»ΡΠ½ΡΡ
ΡΠΈΡΠΎΠΊΠΈΠ½ΠΎΠ² Π² ΠΊΠ»Π΅ΡΠΊΠ°Ρ
ΠΡΠΏΡΠ΅ΡΠ° ΠΏΡΠΎΠΈΡΡ
ΠΎΠ΄ΠΈΡ ΠΌΠ΅Π΄Π»Π΅Π½Π½Π΅Π΅ ΠΏΠΎ ΡΡΠ°Π²Π½Π΅Π½ΠΈΡ Ρ ΠΌΠ°ΠΊΡΠΎΡΠ°Π³Π°ΠΌΠΈ ΠΌΠΎΠ½ΠΎΡΠΈΡΠ°ΡΠ½ΠΎΠ³ΠΎ ΠΏΡΠΎΠΈΡΡ
ΠΎΠΆΠ΄Π΅Π½ΠΈΡ
ΠΠ»ΠΈΡΠ½ΠΈΠ΅ ΡΠΊΡΠΏΠ΅ΡΠΈΠΌΠ΅Π½ΡΠ°Π»ΡΠ½ΠΎΠ³ΠΎ ΠΌΠΎΠ΄Π΅Π»ΠΈΡΠΎΠ²Π°Π½ΠΈΡ ΡΠ΅ΠΏΡΠΎΠΏΠ»Π°ΡΡΠΈΠΊΠΈ Π½Π° ΡΠΈΡΠΎΠ°ΡΡ ΠΈΡΠ΅ΠΊΡΠΎΠ½ΠΈΠΊΡ Π³ΠΈΠΏΠΏΠΎΠΊΠ°ΠΌΠΏΠ° Ρ ΠΊΡΡΡ
Objective: to evaluate the effect of septoplasty modeling on changes in the hippocampal cytoarchitectonics in rats. Material and methods. The study was conducted on 80 sexually mature male rats. In the experimental 1st and 2nd groups, premedication with phenazepamum solution was performed. The first group: 30 rats, local infiltration anesthesia with 2% lidocaine solution; the second group: 30 rats, local infiltration anesthesia with 2% ultracaine solution, postoperative analgesia with sodium diclofenac solution (6 days); the 3rd and 4th groups were control (10 animals each). In groups 1-3, pre-trepanation fixation of the brain was performed, in group 4 this was not done, and artifact dark neurons (DN) were counted. We studied the number of DN in the hippocampus on brain sections stained with hematoxylin-eosin on the 2nd, 6th and 14th days after surgery. Results. In the 2nd group, in the zones CA1, CA2, CA3 and DG, a smaller number of DN was observed compared with the 1st group on the 6th day (p <0.05), and on the 14th day in the 2nd group the number of DN was comparable with the 3rd group in zones CA1 and CA2 (p<0.05). In the 4th group, compared with the 3rd group, the number of DN was significantly higher in all hippocampal zones (p<0.05). Conclusions. Quantitative changes in DN may indicate the effect of surgical stress in the modeling of septoplasty and various anesthesia on changes in cytoarchitectonics in various parts of the hippocampus.Π¦Π΅Π»Ρ: ΠΎΡΠ΅Π½ΠΈΡΡ Π²Π»ΠΈΡΠ½ΠΈΠ΅ ΠΌΠΎΠ΄Π΅Π»ΠΈΡΠΎΠ²Π°Π½ΠΈΡ ΡΠ΅ΠΏΡΠΎΠΏΠ»Π°ΡΡΠΈΠΊΠΈ Π½Π° ΠΈΠ·ΠΌΠ΅Π½Π΅Π½ΠΈΠ΅ ΡΠΈΡΠΎΠ°ΡΡ
ΠΈΡΠ΅ΠΊΡΠΎΠ½ΠΈΠΊΠΈ Π³ΠΈΠΏΠΏΠΎΠΊΠ°ΠΌΠΏΠ° Ρ ΠΊΡΡΡ. ΠΠ°ΡΠ΅ΡΠΈΠ°Π» ΠΈ ΠΌΠ΅ΡΠΎΠ΄Ρ. ΠΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠ΅ ΠΏΡΠΎΠ²ΠΎΠ΄ΠΈΠ»ΠΎΡΡ Π½Π° 80 ΠΏΠΎΠ»ΠΎΠ²ΠΎΠ·ΡΠ΅Π»ΡΡ
ΠΊΡΡΡΠ°Ρ
ΡΠ°ΠΌΡΠ°Ρ
. Π ΡΠΊΡΠΏΠ΅ΡΠΈΠΌΠ΅Π½ΡΠ°Π»ΡΠ½ΡΡ
1-ΠΉ ΠΈ 2-ΠΉ Π³ΡΡΠΏΠΏΠ°Ρ
ΠΏΡΠΎΠ²ΠΎΠ΄ΠΈΠ»Π°ΡΡ ΠΏΡΠ΅ΠΌΠ΅Π΄ΠΈΠΊΠ°ΡΠΈΡ ΡΠ°ΡΡΠ²ΠΎΡΠΎΠΌ ΡΠ΅Π½ΠΎΠ·Π΅ΠΏΠ°ΠΌΠ°. ΠΠ΅ΡΠ²Π°Ρ Π³ΡΡΠΏΠΏΠ°: 30 ΠΊΡΡΡ, ΠΌΠ΅ΡΡΠ½Π°Ρ ΠΈΠ½ΡΠΈΠ»ΡΡΡΠ°ΡΠΈΠΎΠ½Π½Π°Ρ Π°Π½Π΅ΡΡΠ΅Π·ΠΈΡ 2% ΡΠ°ΡΡΠ²ΠΎΡΠΎΠΌ Π»ΠΈΠ΄ΠΎΠΊΠ°ΠΈΠ½Π°; 2-Ρ Π³ΡΡΠΏΠΏΠ°: 30 ΠΊΡΡΡ, ΠΌΠ΅ΡΡΠ½Π°Ρ ΠΈΠ½ΡΠΈΠ»ΡΡΡΠ°ΡΠΈΠΎΠ½Π½Π°Ρ Π°Π½Π΅ΡΡΠ΅Π·ΠΈΡ 2% ΡΠ°ΡΡΠ²ΠΎΡΠΎΠΌ ΡΠ»ΡΡΡΠ°ΠΊΠ°ΠΈΠ½Π°, ΠΏΠΎΡΠ»Π΅ΠΎΠΏΠ΅ΡΠ°ΡΠΈΠΎΠ½Π½Π°Ρ Π°Π½Π°Π»ΡΠ³Π΅Π·ΠΈΡ ΡΠ°ΡΡΠ²ΠΎΡΠΎΠΌ Π΄ΠΈΠΊΠ»ΠΎΡΠ΅Π½Π°ΠΊΠ° Π½Π°ΡΡΠΈΡ (6 Π΄Π½Π΅ΠΉ); 3-Ρ ΠΈ 4-Ρ Π³ΡΡΠΏΠΏΡ Π±ΡΠ»ΠΈ ΠΊΠΎΠ½ΡΡΠΎΠ»ΡΠ½ΡΠΌΠΈ (ΠΏΠΎ 10 ΠΆΠΈΠ²ΠΎΡΠ½ΡΡ
). Π 1-3-ΠΉ Π³ΡΡΠΏΠΏΠ°Ρ
ΠΏΡΠΎΠ²ΠΎΠ΄ΠΈΠ»Π°ΡΡ ΠΏΡΠ΅Π΄ΡΡΠ΅ΠΏΠ°Π½Π°ΡΠΈΠΎΠ½Π½Π°Ρ ΡΠΈΠΊΡΠ°ΡΠΈΡ Π³ΠΎΠ»ΠΎΠ²Π½ΠΎΠ³ΠΎ ΠΌΠΎΠ·Π³Π°, Π² 4-ΠΉ Π³ΡΡΠΏΠΏΠ΅ ΡΡΠΎ Π½Π΅ ΠΏΡΠΎΠ²ΠΎΠ΄ΠΈΠ»ΠΈ, Π° ΠΏΠΎΠ΄ΡΡΠΈΡΡΠ²Π°Π»ΠΈ Π°ΡΡΠ΅ΡΠ°ΠΊΡΠ½ΡΠ΅ ΡΠ΅ΠΌΠ½ΡΠ΅ Π½Π΅ΠΉΡΠΎΠ½Ρ (Π’Π). ΠΠ·ΡΡΠ°Π»ΠΈ ΡΠΈΡΠ»ΠΎ Π’Π Π² Π³ΠΈΠΏΠΏΠΎΠΊΠ°ΠΌΠΏΠ΅ Π½Π° ΡΡΠ΅Π·Π°Ρ
Π³ΠΎΠ»ΠΎΠ²Π½ΠΎΠ³ΠΎ ΠΌΠΎΠ·Π³Π°, ΠΎΠΊΡΠ°ΡΠ΅Π½Π½ΡΡ
Π³Π΅ΠΌΠ°ΡΠΎΠΊΡΠΈΠ»ΠΈΠ½-ΡΠΎΠ·ΠΈΠ½ΠΎΠΌ, Π½Π° 2-ΠΉ, 6 ΠΈ 14-ΠΉ Π΄Π½ΠΈ ΠΏΠΎΡΠ»Π΅ ΠΎΠΏΠ΅ΡΠ°ΡΠΈΠΈ. Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ. ΠΠΎ 2-ΠΉ Π³ΡΡΠΏΠΏΠ΅ Π² Π·ΠΎΠ½Π°Ρ
Π‘Π1, Π‘Π2, Π‘Π3 ΠΈ DG Π½Π°Π±Π»ΡΠ΄Π°Π»ΠΎΡΡ ΠΌΠ΅Π½ΡΡΠ΅Π΅ ΡΠΈΡΠ»ΠΎ Π’Π ΠΏΠΎ ΡΡΠ°Π²Π½Π΅Π½ΠΈΡ Ρ 1-ΠΉ Π³ΡΡΠΏΠΏΠΎΠΉ Π½Π° 6-ΠΉ Π΄Π΅Π½Ρ (p<0,05), Π° Π½Π° 14-ΠΉ Π΄Π΅Π½Ρ Π²ΠΎ 2-ΠΉ Π³ΡΡΠΏΠΏΠ΅ ΡΠΈΡΠ»ΠΎ Π’Π Π±ΡΠ»ΠΎ ΡΠΎΠΏΠΎΡΡΠ°Π²ΠΈΠΌΠΎ Ρ 3-ΠΉ Π³ΡΡΠΏΠΏΠΎΠΉ Π² Π·ΠΎΠ½Π°Ρ
Π‘Π1 ΠΈ Π‘Π2 (p<0,05). Π 4-ΠΉ Π³ΡΡΠΏΠΏΠ΅ ΠΏΠΎ ΡΡΠ°Π²Π½Π΅Π½ΠΈΡ Ρ 3-ΠΉ Π³ΡΡΠΏΠΏΠΎΠΉ ΡΠΈΡΠ»ΠΎ Π’Π Π±ΡΠ»ΠΎ Π΄ΠΎΡΡΠΎΠ²Π΅ΡΠ½ΠΎ Π²ΡΡΠ΅ Π²ΠΎ Π²ΡΠ΅Ρ
Π³ΠΈΠΏΠΏΠΎΠΊΠ°ΠΌΠΏΠ°Π»ΡΠ½ΡΡ
Π·ΠΎΠ½Π°Ρ
(p<0,05). ΠΡΠ²ΠΎΠ΄Ρ. ΠΠΎΠ»ΠΈΡΠ΅ΡΡΠ²Π΅Π½Π½ΡΠ΅ ΠΈΠ·ΠΌΠ΅Π½Π΅Π½ΠΈΡ Π’Π ΠΌΠΎΠ³ΡΡ ΡΠ²ΠΈΠ΄Π΅ΡΠ΅Π»ΡΡΡΠ²ΠΎΠ²Π°ΡΡ ΠΎ Π²Π»ΠΈΡΠ½ΠΈΠΈ Ρ
ΠΈΡΡΡΠ³ΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΡΡΡΠ΅ΡΡΠ° ΠΏΡΠΈ ΠΌΠΎΠ΄Π΅Π»ΠΈΡΠΎΠ²Π°Π½ΠΈΠΈ ΡΠ΅ΠΏΡΠΎΠΏΠ»Π°ΡΡΠΈΠΊΠΈ ΠΈ ΡΠ°Π·Π»ΠΈΡΠ½ΠΎΠΌ Π°Π½Π΅ΡΡΠ΅Π·ΠΈΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΎΠΌ ΠΏΠΎΡΠΎΠ±ΠΈΠΈ Π½Π° ΠΈΠ·ΠΌΠ΅Π½Π΅Π½ΠΈΡ ΡΠΈΡΠΎΠ°ΡΡ
ΠΈΡΠ΅ΠΊΡΠΎΠ½ΠΈΠΊΠΈ Π² ΡΠ°Π·Π»ΠΈΡΠ½ΡΡ
ΠΎΡΠ΄Π΅Π»Π°Ρ
Π³ΠΈΠΏΠΏΠΎΠΊΠ°ΠΌΠΏΠ°
ΠΠΠ£Π§ΠΠΠΠ ΠΠΠΠ―ΠΠΠ― Π‘Π’Π ΠΠ‘Π‘Π ΠΠ ΠΠΠ Π€ΠΠΠΠΠΠ§ΠΠ‘ΠΠΠ ΠΠΠΠΠΠΠΠΠ― Π ΠΠΠΠΠΠΠΠΠΠ ΠΠ Π Π₯ΠΠ Π£Π ΠΠΠ§ΠΠ‘ΠΠΠ₯ ΠΠΠΠ¨ΠΠ’ΠΠΠ¬Π‘Π’ΠΠΠ₯ Π ΠΠΠΠΠ‘Π’Π ΠΠΠ‘Π
Objective - to study the morphological changes in the hippocampus of the rat brain following modeling septoplasty. Material and methods. The study was conducted on 80 adult male rats. In the experimental groups1 and 2, premedica tion with Phenazepamum was carried out. Group 1 included 30 rats subjected to local infiltration anesthesia with 2 % lidocaine solution; group 2 included 30 rats subjected to local infiltration anesthesia with 2 % solution of articain & epinephrine (0,5 mg/100 ml) and postoperative analgesia with diclofenac sodium solution for 6 days after surgery. Groups 3 and 4 were controls and included 10 animals each. In groups 1-3, fixation of the brain was performed prior to trepanation; in group 4, similar fixation was not performed and artifact dark neurons were counted. The number of dark neurons in the hippocampus was counted on hematoxylin-eosin stained brain sections on days 2, 6 and 14 after surgery. Results. In group 2, in the CA1, CA2, CA3 and DG zones, there were fewer dark neurons (DN) on day 6 compared with group 1. On 14th day, the number of DN in areas CA1 and CA2 was comparable in groups 2 and 3. In group 4, compared with group 3, the number of DN was greater in all studied hippocampal zones. Conclusion. The quantitative changes of DN in different zones of the hippocampus may indicate the severity of surgical stress following simulation of septoplasty under different types of anesthesia.Π¦Π΅Π»Ρ - ΠΈΠ·ΡΡΠΈΡΡ ΠΌΠΎΡΡΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΠ΅ ΠΈΠ·ΠΌΠ΅Π½Π΅Π½ΠΈΡ Π² Π³ΠΈΠΏΠΏΠΎΠΊΠ°ΠΌΠΏΠ΅ ΠΌΠΎΠ·Π³Π° ΠΊΡΡΡ ΠΏΡΠΈ ΠΌΠΎΠ΄Π΅Π»ΠΈΡΠΎΠ²Π°Π½ΠΈΠΈ ΠΏΠ»Π°ΡΡΠΈΠΊΠΈ Π½ΠΎΡΠΎΠ²ΠΎΠΉ ΠΏΠ΅ΡΠ΅Π³ΠΎΡΠΎΠ΄ΠΊΠΈ. ΠΠ°ΡΠ΅ΡΠΈΠ°Π»Ρ ΠΈ ΠΌΠ΅ΡΠΎΠ΄Ρ. Π Π°Π±ΠΎΡΠ° Π²ΡΠΏΠΎΠ»Π½Π΅Π½Π° Π½Π° 80 ΠΏΠΎΠ»ΠΎΠ²ΠΎΠ·ΡΠ΅Π»ΡΡ
ΠΊΡΡΡΠ°Ρ
-ΡΠ°ΠΌΡΠ°Ρ
. Π ΡΠΊΡΠΏΠ΅ΡΠΈΠΌΠ΅Π½ΡΠ°Π»ΡΠ½ΡΡ
1-ΠΉΠΈ 2-ΠΉ Π³ΡΡΠΏΠΏΠ΅ ΠΏΡΠΎΠ²ΠΎΠ΄ΠΈΠ»ΠΈ ΠΏΡΠ΅ΠΌΠ΅Π΄ΠΈΠΊΠ°ΡΠΈΡ ΡΠ°ΡΡΠ²ΠΎΡΠΎΠΌ ΡΠ΅Π½ΠΎΠ·Π΅ΠΏΠ°ΠΌΠ°. 1-Ρ Π³ΡΡΠΏΠΏΠ° Π²ΠΊΠ»ΡΡΠ°Π»Π° 30 ΠΊΡΡΡ, ΠΏΠΎΠ΄Π²Π΅ΡΠ³Π½ΡΡΡΡ
ΠΌΠ΅ΡΡΠ½ΠΎΠΉ ΠΈΠ½ΡΠΈΠ»ΡΡΡΠ°ΡΠΈΠΎΠ½Π½ΠΎΠΉ Π°Π½Π΅ΡΡΠ΅Π·ΠΈΠΈ 2 % ΡΠ°ΡΡΠ²ΠΎΡΠΎΠΌ Π»ΠΈΠ΄ΠΎΠΊΠ°ΠΈΠ½Π°; 2-Ρ Π³ΡΡΠΏΠΏΠ° - 30 ΠΊΡΡΡ, ΠΏΠΎΠ΄Π²Π΅ΡΠ³Π½ΡΡΡΡ
ΠΌΠ΅ΡΡΠ½ΠΎΠΉ ΠΈΠ½ΡΠΈΠ»ΡΡΡΠ°ΡΠΈΠΎΠ½Π½ΠΎΠΉ Π°Π½Π΅ΡΡΠ΅Π·ΠΈΠΈ 2 % ΡΠ°ΡΡΠ²ΠΎΡΠΎΠΌ Π°ΡΡΠΈΠΊΠ°ΠΈΠ½Π° ΠΈ ΡΠΏΠΈΠ½Π΅ΡΡΠΈΠ½Π° (0,5 ΠΌΠ³/100 ΠΌΠ»), ΠΏΠΎΡΠ»Π΅ΠΎΠΏΠ΅ΡΠ°ΡΠΈΠΎΠ½Π½ΠΎΠΉ Π°Π½Π°Π»Π³Π΅Π·ΠΈΠΈ ΡΠ°ΡΡΠ²ΠΎΡΠΎΠΌ Π΄ΠΈΠΊΠ»ΠΎΡΠ΅Π½Π°ΠΊΠ° Π½Π°ΡΡΠΈΡ Π² ΡΠ΅ΡΠ΅Π½ΠΈΠ΅ 6 ΡΡΡ ΠΏΠΎΡΠ»Π΅ ΠΎΠΏΠ΅ΡΠ°ΡΠΈΠΈ. 3-ΡΠΈ 4-Ρ Π³ΡΡΠΏΠΏΠ° Π±ΡΠ»ΠΈ ΠΊΠΎΠ½ΡΡΠΎΠ»ΡΠ½ΡΠΌΠΈ ΠΈ Π²ΠΊΠ»ΡΡΠ°Π»ΠΈ ΠΏΠΎ 10 ΠΆΠΈΠ²ΠΎΡΠ½ΡΡ
. Π 1-3-ΠΉ Π³ΡΡΠΏΠΏΠ΅ ΠΏΡΠΎΠ²ΠΎΠ΄ΠΈΠ»Π°ΡΡ ΠΏΡΠ΅Π΄ΡΡΠ΅ΠΏΠ°Π½Π°ΡΠΈΠΎΠ½Π½Π°Ρ ΡΠΈΠΊΡΠ°ΡΠΈΡ Π³ΠΎΠ»ΠΎΠ²Π½ΠΎΠ³ΠΎ ΠΌΠΎΠ·Π³Π°, Π² 4-ΠΉ Π³ΡΡΠΏΠΏΠ΅ ΠΏΠΎΠ΄ΠΎΠ±Π½ΠΎΠΉ ΡΠΈΠΊΡΠ°ΡΠΈΠΈ Π½Π΅ ΠΏΡΠΎΠ²ΠΎΠ΄ΠΈΠ»ΠΎΡΡ ΠΈ ΠΏΠΎΠ΄ΡΡΠΈΡΡΠ²Π°Π»ΠΈΡΡ Π°ΡΡΠ΅ΡΠ°ΠΊΡΠ½ΡΠ΅ ΡΠ΅ΠΌΠ½ΡΠ΅ Π½Π΅ΠΉΡΠΎΠ½Ρ (Π’Π). ΠΠ·ΡΡΠ°Π»ΠΈ ΡΠΈΡΠ»ΠΎ Π’Π Π² Π³ΠΈΠΏΠΏΠΎΠΊΠ°ΠΌΠΏΠ΅ Π½Π° ΡΡΠ΅Π·Π°Ρ
Π³ΠΎΠ»ΠΎΠ²Π½ΠΎΠ³ΠΎ ΠΌΠΎΠ·Π³Π°, ΠΎΠΊΡΠ°ΡΠ΅Π½Π½ΡΡ
Π³Π΅ΠΌΠ°ΡΠΎΠΊΡΠΈΠ»ΠΈΠ½ΠΎΠΌ ΠΈ ΡΠΎΠ·ΠΈΠ½ΠΎΠΌ, Π½Π° 2-, 6-Π΅ΠΈ 14-Π΅ ΡΡΡΠΊΠΈ ΠΏΠΎΡΠ»Π΅ ΠΎΠΏΠ΅ΡΠ°ΡΠΈΠΈ. Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ. ΠΠΎ 2-ΠΉ Π³ΡΡΠΏΠΏΠ΅ Π² Π·ΠΎΠ½Π°Ρ
Π‘Π1, Π‘Π2, Π‘Π3 ΠΈ DG Π½Π°Π±Π»ΡΠ΄Π°Π»ΠΈ ΠΌΠ΅Π½ΡΡΠ΅Π΅ ΡΠΈΡΠ»ΠΎ Π’Π ΠΏΠΎ ΡΡΠ°Π²Π½Π΅Π½ΠΈΡ Ρ 1-ΠΉ Π³ΡΡΠΏΠΏΠΎΠΉ Π½Π° 6-Π΅ ΡΡΡΠΊΠΈ. ΠΠ° 14-Π΅ ΡΡΡΠΊΠΈ ΡΠΈΡΠ»ΠΎ Π’Π Π²ΠΎ 2-ΠΉΠΈ 3-ΠΉ Π³ΡΡΠΏΠΏΠ΅ Π±ΡΠ»ΠΎ ΡΠΎΠΏΠΎΡΡΠ°Π²ΠΈΠΌΠΎ Π² Π·ΠΎΠ½Π°Ρ
Π‘Π1 ΠΈ Π‘Π2. Π 4-ΠΉ Π³ΡΡΠΏΠΏΠ΅ ΠΏΠΎ ΡΡΠ°Π²Π½Π΅Π½ΠΈΡ Ρ 3-ΠΉ Π³ΡΡΠΏΠΏΠΎΠΉ ΡΠΈΡΠ»ΠΎ Π’Π Π±ΡΠ»ΠΎ Π±ΠΎΠ»ΡΡΠ΅ Π²ΠΎ Π²ΡΠ΅Ρ
ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½Π½ΡΡ
Π·ΠΎΠ½Π°Ρ
Π³ΠΈΠΏΠΏΠΎΠΊΠ°ΠΌΠΏΠ°. ΠΡΠ²ΠΎΠ΄. ΠΠΎΠ»ΠΈΡΠ΅ΡΡΠ²Π΅Π½Π½ΡΠ΅ ΠΈΠ·ΠΌΠ΅Π½Π΅Π½ΠΈΡ Π’Π Π² ΡΠ°Π·Π»ΠΈΡΠ½ΡΡ
Π·ΠΎΠ½Π°Ρ
Π³ΠΈΠΏΠΏΠΎΠΊΠ°ΠΌΠΏΠ° ΠΌΠΎΠ³ΡΡ ΡΠ²ΠΈΠ΄Π΅ΡΠ΅Π»ΡΡΡΠ²ΠΎΠ²Π°ΡΡ ΠΎ Π²ΡΡΠ°ΠΆΠ΅Π½Π½ΠΎΠΌ Ρ
ΠΈΡΡΡΠ³ΠΈΡΠ΅ΡΠΊΠΎΠΌ ΡΡΡΠ΅ΡΡΠ΅ Π²ΠΎ Π²ΡΠ΅ΠΌΡ ΠΌΠΎΠ΄Π΅Π»ΠΈΡΠΎΠ²Π°Π½ΠΈΡ ΡΠ΅ΠΏΡΠΎΠΏΠ»Π°ΡΡΠΈΠΊΠΈ ΠΏΡΠΈ ΡΠ°Π·Π»ΠΈΡΠ½ΠΎΠΌ Π°Π½Π΅ΡΡΠ΅Π·ΠΈΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΎΠΌ ΠΏΠΎΡΠΎΠ±ΠΈΠΈ