11 research outputs found
ΠΠΌΠΌΡΠ½ΠΎΠ³ΠΈΡΡΠΎΡ ΠΈΠΌΠΈΡΠ΅ΡΠΊΠΎΠ΅ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠ΅ ΠΏΡΠΎΠ»ΠΈΡΠ΅ΡΠ°ΡΠΈΠ²Π½ΠΎΠΉ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ ΠΎΠΏΡΡ ΠΎΠ»Π΅Π²ΡΡ ΠΊΠ»Π΅ΡΠΎΠΊ ΠΏΡΠΈ ΡΠ°Π·Π»ΠΈΡΠ½ΡΡ Π³ΡΠ°Π΄Π°ΡΠΈΡΡ Π°Π΄Π΅Π½ΠΎΠΊΠ°ΡΡΠΈΠ½ΠΎΠΌΡ ΠΏΡΠ΅Π΄ΡΡΠ°ΡΠ΅Π»ΡΠ½ΠΎΠΉ ΠΆΠ΅Π»Π΅Π·Ρ
Prostate cancer is one of the leading causes of men death in developed countries. Modern diagnostic methods, including a puncture biopsy of the prostate gland, make it possible to verify oncology in the early stages, however, routine studies do not always allow to predict the course of the disease and outcome. The aim of our study was to analyze the clinical, morphological and immunohistochemical features of the proliferative activity of adenocarcinoma cells using the Ki-67 marker, to compare the degree of proliferative activity in tumors of various degrees of malignancy (according to Gleasonβs classification), as well as to compare this indicator with the clinical stage of the disease, the level of prostate-specific antigen in the blood, the size of the prostate gland. Materials and Methods: On the basis of the City Clinical Hospital No. 31 and the Veteran Hospital No. 2, paraffin blocks with material obtained as a result of prostate biopsy, transurethral resection, and radical prostatectomy were selected. A morphological and immunohistochemical study of the material with the Ki-67 marker and a quantitative assessment of the degree of proliferative activity were carried out. Data were analyzed using the STATISTICA 10.0 program using estimates of the normality of the data distribution according to the Shapiro-Wilk W-test, the significance of differences was estimated using the Mann-Whitney U-test, and correlation relationships using Spearmanβs correlation coefficient. Results: Statistically significant differences in the degree of proliferative activity in groups differing in the degree of differentiation were revealed. A statistically significant direct correlation of moderate severity was revealed when comparing proliferative activity with the degree of differentiation according to the Gleason system (rs = 0.523) and the clinical stage of the disease (rs = 0.646). No statistically significant correlation was found between indicators such as prostate-specific antigen level, age, prostate volume, and proliferative activity index. Conclusion: taking into account the proliferative activity index in addition to clinical and morphological studies helps to diagnose and subsequently predict the course of prostate cancer.ΠΠΊΡΡΠ°Π»ΡΠ½ΠΎΡΡΡ. Π Π°ΠΊ ΠΏΡΠ΅Π΄ΡΡΠ°ΡΠ΅Π»ΡΠ½ΠΎΠΉ ΠΆΠ΅Π»Π΅Π·Ρ ΡΠ²Π»ΡΠ΅ΡΡΡ ΠΎΠ΄Π½ΠΎΠΉ ΠΈΠ· Π²Π΅Π΄ΡΡΠΈΡ
ΠΏΡΠΈΡΠΈΠ½ ΡΠΌΠ΅ΡΡΠΈ ΠΌΡΠΆΡΠΊΠΎΠ³ΠΎ Π½Π°ΡΠ΅Π»Π΅Π½ΠΈΡ ΠΎΡ ΠΎΠ½ΠΊΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΡ
Π·Π°Π±ΠΎΠ»Π΅Π²Π°Π½ΠΈΠΉ Π² ΡΠ°Π·Π²ΠΈΡΡΡ
ΡΡΡΠ°Π½Π°Ρ
. Π‘ΠΎΠ²ΡΠ΅ΠΌΠ΅Π½Π½ΡΠ΅ ΠΌΠ΅ΡΠΎΠ΄Ρ Π΄ΠΈΠ°Π³Π½ΠΎΡΡΠΈΠΊΠΈ, Π² ΡΠΎΠΌ ΡΠΈΡΠ»Π΅ ΠΏΡΠ½ΠΊΡΠΈΠΎΠ½Π½Π°Ρ Π±ΠΈΠΎΠΏΡΠΈΡ ΠΏΡΠ΅Π΄ΡΡΠ°ΡΠ΅Π»ΡΠ½ΠΎΠΉ ΠΆΠ΅Π»Π΅Π·Ρ, ΠΏΠΎΠ·Π²ΠΎΠ»ΡΡΡ Π²Π΅ΡΠΈΡΠΈΡΠΈΡΠΎΠ²Π°ΡΡ ΠΎΠ½ΠΊΠΎΠ»ΠΎΠ³ΠΈΡ Π½Π° ΡΠ°Π½Π½ΠΈΡ
ΡΡΠ°Π΄ΠΈΡΡ
, ΠΎΠ΄Π½Π°ΠΊΠΎ ΡΡΡΠΈΠ½Π½ΡΠ΅ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ Π½Π΅ Π²ΡΠ΅Π³Π΄Π° ΠΏΠΎΠ·Π²ΠΎΠ»ΡΡΡ ΡΠΏΡΠΎΠ³Π½ΠΎΠ·ΠΈΡΠΎΠ²Π°ΡΡ ΡΠ΅ΡΠ΅Π½ΠΈΠ΅ ΠΈ ΠΈΡΡ
ΠΎΠ΄ Π·Π°Π±ΠΎΠ»Π΅Π²Π°Π½ΠΈΡ. Π¦Π΅Π»Ρ: ΠΏΡΠΎΠ²Π΅ΡΡΠΈ Π°Π½Π°Π»ΠΈΠ· ΠΊΠ»ΠΈΠ½ΠΈΠΊΠΎ-ΠΌΠΎΡΡΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΈ ΠΈΠΌΠΌΡΠ½ΠΎΠ³ΠΈΡΡΠΎΡ
ΠΈΠΌΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΎΡΠΎΠ±Π΅Π½Π½ΠΎΡΡΠ΅ΠΉ ΠΊΠ»Π΅ΡΠΎΠΊ Π°Π΄Π΅Π½ΠΎΠΊΠ°ΡΡΠΈΠ½ΠΎΠΌΡ, Ρ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ΠΌ ΠΌΠ°ΡΠΊΠ΅ΡΠ° Ki-67, ΡΠΎΠΏΠΎΡΡΠ°Π²Π»Π΅Π½ΠΈΠ΅ ΡΡΠ΅ΠΏΠ΅Π½ΠΈ ΠΏΡΠΎΠ»ΠΈΡΠ΅ΡΠ°ΡΠΈΠ²Π½ΠΎΠΉ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ Π² ΠΎΠΏΡΡ
ΠΎΠ»ΡΡ
ΡΠ°Π·Π»ΠΈΡΠ½ΠΎΠΉ ΡΡΠ΅ΠΏΠ΅Π½ΠΈ Π·Π»ΠΎΠΊΠ°ΡΠ΅ΡΡΠ²Π΅Π½Π½ΠΎΡΡΠΈ (ΠΏΠΎ ΠΊΠ»Π°ΡΡΠΈΡΠΈΠΊΠ°ΡΠΈΠΈ ΠΠ»ΠΈΡΠΎΠ½Π°), Π° ΡΠ°ΠΊΠΆΠ΅ ΡΡΠ°Π²Π½Π΅Π½ΠΈΠ΅ Π΄Π°Π½Π½ΠΎΠ³ΠΎ ΠΏΠΎΠΊΠ°Π·Π°ΡΠ΅Π»Ρ Ρ ΠΊΠ»ΠΈΠ½ΠΈΡΠ΅ΡΠΊΠΎΠΉ ΡΡΠ°Π΄ΠΈΠ΅ΠΉ Π·Π°Π±ΠΎΠ»Π΅Π²Π°Π½ΠΈΡ, ΡΡΠΎΠ²Π½Π΅ΠΌ ΠΏΡΠΎΡΡΠ°ΡΡΠΏΠ΅ΡΠΈΡΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ Π°Π½ΡΠΈΠ³Π΅Π½Π° Π² ΠΊΡΠΎΠ²ΠΈ, ΠΎΠ±ΡΠ΅ΠΌΠΎΠΌ ΠΏΡΠ΅Π΄ΡΡΠ°ΡΠ΅Π»ΡΠ½ΠΎΠΉ ΠΆΠ΅Π»Π΅Π·Ρ. ΠΠ°ΡΠ΅ΡΠΈΠ°Π»Ρ ΠΈ ΠΌΠ΅ΡΠΎΠ΄Ρ: ΠΠ° Π±Π°Π·Π΅ ΠΠΠ£Π ΠΠΠ Β«ΠΠΎΡΠΎΠ΄ΡΠΊΠ°Ρ ΠΊΠ»ΠΈΠ½ΠΈΡΠ΅ΡΠΊΠ°Ρ Π±ΠΎΠ»ΡΠ½ΠΈΡΠ° β 31Β» ΠΈ Β«ΠΠΎΡΠΏΠΈΡΠ°Π»Ρ Π΄Π»Ρ Π²Π΅ΡΠ΅ΡΠ°Π½ΠΎΠ² Π²ΠΎΠΈΠ½ β 2Β» Π±ΡΠ»ΠΈ ΠΎΡΠΎΠ±ΡΠ°Π½Ρ ΠΏΠ°ΡΠ°ΡΠΈΠ½ΠΎΠ²ΡΠ΅ Π±Π»ΠΎΠΊΠΈ Ρ ΠΌΠ°ΡΠ΅ΡΠΈΠ°Π»ΠΎΠΌ, ΠΏΠΎΠ»ΡΡΠ΅Π½Π½ΡΠΌ Π² ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΠ΅ Π±ΠΈΠΎΠΏΡΠΈΠΈ ΠΏΡΠ΅Π΄ΡΡΠ°ΡΠ΅Π»ΡΠ½ΠΎΠΉ ΠΆΠ΅Π»Π΅Π·Ρ, ΡΡΠ°Π½ΡΡΡΠ΅ΡΡΠ°Π»ΡΠ½ΠΎΠΉ ΡΠ΅Π·Π΅ΠΊΡΠΈΠΈ ΠΈ ΡΠ°Π΄ΠΈΠΊΠ°Π»ΡΠ½ΠΎΠΉ ΠΏΡΠΎΡΡΠ°ΡΡΠΊΡΠΎΠΌΠΈΠΈ. ΠΡΠΎΠ²Π΅Π΄Π΅Π½ΠΎ ΠΌΠΎΡΡΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΎΠ΅ ΠΈ ΠΈΠΌΠΌΡΠ½ΠΎΠ³ΠΈΡΡΠΎΡ
ΠΈΠΌΠΈΡΠ΅ΡΠΊΠΎΠ΅ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠ΅ ΠΌΠ°ΡΠ΅ΡΠΈΠ°Π»Π° Ρ ΠΌΠ°ΡΠΊΠ΅ΡΠΎΠΌ Ki-67. ΠΠΎΠ»ΡΡΠ΅Π½Π½ΡΠ΅ Π΄Π°Π½Π½ΡΠ΅ ΠΏΡΠΎΠ°Π½Π°Π»ΠΈΠ·ΠΈΡΠΎΠ²Π°Π½Ρ ΠΏΡΠΈ ΠΏΠΎΠΌΠΎΡΠΈ ΠΏΡΠΎΠ³ΡΠ°ΠΌΠΌΡ STATISTICA 10.0 Ρ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ΠΌ ΠΎΡΠ΅Π½ΠΊΠΈ Π½ΠΎΡΠΌΠ°Π»ΡΠ½ΠΎΡΡΠΈ ΡΠ°ΡΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΡ Π΄Π°Π½Π½ΡΡ
ΠΏΠΎ W-ΠΊΡΠΈΡΠ΅ΡΠΈΡ Π¨Π°ΠΏΠΈΡΠΎ-Π£ΠΈΠ»ΠΊΠ°, Π΄ΠΎΡΡΠΎΠ²Π΅ΡΠ½ΠΎΡΡΡ ΡΠ°Π·Π»ΠΈΡΠΈΠΉ ΠΎΡΠ΅Π½ΠΈΠ²Π°Π»Π°ΡΡ Ρ ΠΏΠΎΠΌΠΎΡΡΡ U-ΠΊΡΠΈΡΠ΅ΡΠΈΡ ΠΠ°Π½Π½Π°-Π£ΠΈΡΠ½ΠΈ, Π° ΠΊΠΎΡΡΠ΅Π»ΡΡΠΈΠΎΠ½Π½ΡΠ΅ Π²Π·Π°ΠΈΠΌΠΎΠΎΡΠ½ΠΎΡΠ΅Π½ΠΈΡ Ρ ΠΏΠΎΠΌΠΎΡΡΡ ΠΊΠΎΡΡΡΠΈΡΠΈΠ΅Π½ΡΠ° ΠΊΠΎΡΡΠ΅Π»ΡΡΠΈΠΈ Π‘ΠΏΠΈΡΠΌΠ΅Π½Π°. Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ: ΠΡΡΠ²Π»Π΅Π½Ρ ΡΡΠ°ΡΠΈΡΡΠΈΡΠ΅ΡΠΊΠΈ Π·Π½Π°ΡΠΈΠΌΡΠ΅ ΠΎΡΠ»ΠΈΡΠΈΡ ΠΏΡΠΎΠ»ΠΈΡΠ΅ΡΠ°ΡΠΈΠ²Π½ΠΎΠΉ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ Π² Π³ΡΡΠΏΠΏΠ°Ρ
Π·Π»ΠΎΠΊΠ°ΡΠ΅ΡΡΠ²Π΅Π½Π½ΠΎΠΉ ΠΎΠΏΡΡ
ΠΎΠ»ΠΈ Ρ ΡΠ°Π·Π½ΠΎΠΉ ΡΡΠ΅ΠΏΠ΅Π½ΡΡ Π΄ΠΈΡΡΠ΅ΡΠ΅Π½ΡΠΈΡΠΎΠ²ΠΊΠΈ. Π‘ΡΠ°ΡΠΈΡΡΠΈΡΠ΅ΡΠΊΠΈ Π·Π½Π°ΡΠΈΠΌΠ°Ρ ΠΏΡΡΠΌΠ°Ρ ΠΊΠΎΡΡΠ΅Π»ΡΡΠΈΡ ΡΠΌΠ΅ΡΠ΅Π½Π½ΠΎΠΉ Π²ΡΡΠ°ΠΆΠ΅Π½Π½ΠΎΡΡΠΈ Π±ΡΠ»Π° Π²ΡΡΠ²Π»Π΅Π½Π° ΠΏΡΠΈ ΡΠΎΠΏΠΎΡΡΠ°Π²Π»Π΅Π½ΠΈΠΈ ΠΏΡΠΎΠ»ΠΈΡΠ΅ΡΠ°ΡΠΈΠ²Π½ΠΎΠΉ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ ΡΠΎ ΡΡΠ΅ΠΏΠ΅Π½ΡΡ Π΄ΠΈΡΡΠ΅ΡΠ΅Π½ΡΠΈΡΠΎΠ²ΠΊΠΈ ΠΏΠΎ ΡΠΈΡΡΠ΅ΠΌΠ΅ ΠΠ»ΠΈΡΠΎΠ½Π° (rs = 0,523) ΠΈ ΠΊΠ»ΠΈΠ½ΠΈΡΠ΅ΡΠΊΠΎΠΉ ΡΡΠ°Π΄ΠΈΠ΅ΠΉ Π·Π°Π±ΠΎΠ»Π΅Π²Π°Π½ΠΈΡ (rs = 0,646). ΠΠ΅ΠΆΠ΄Ρ ΡΠ°ΠΊΠΈΠΌΠΈ ΠΏΠΎΠΊΠ°Π·Π°ΡΠ΅Π»ΡΠΌΠΈ, ΠΊΠ°ΠΊ ΡΡΠΎΠ²Π΅Π½Ρ ΠΏΡΠΎΡΡΠ°ΡΡΠΏΠ΅ΡΠΈΡΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ Π°Π½ΡΠΈΠ³Π΅Π½Π°, Π²ΠΎΠ·ΡΠ°ΡΡ, ΠΎΠ±ΡΠ΅ΠΌ ΠΏΡΠ΅Π΄ΡΡΠ°ΡΠ΅Π»ΡΠ½ΠΎΠΉ ΠΆΠ΅Π»Π΅Π·Ρ ΠΈ ΠΈΠ½Π΄Π΅ΠΊΡΠΎΠΌ ΠΏΡΠΎΠ»ΠΈΡΠ΅ΡΠ°ΡΠΈΠ²Π½ΠΎΠΉ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ ΡΡΠ°ΡΠΈΡΡΠΈΡΠ΅ΡΠΊΠΈ Π·Π½Π°ΡΠΈΠΌΡΡ
ΠΊΠΎΡΡΠ΅Π»ΡΡΠΈΠΎΠ½Π½ΡΡ
Π·Π°Π²ΠΈΡΠΈΠΌΠΎΡΡΠ΅ΠΉ ΠΎΠ±Π½Π°ΡΡΠΆΠ΅Π½ΠΎ Π½Π΅ Π±ΡΠ»ΠΎ. ΠΠ°ΠΊΠ»ΡΡΠ΅Π½ΠΈΠ΅: ΡΡΠ΅Ρ ΠΈΠ½Π΄Π΅ΠΊΡΠ° ΠΏΡΠΎΠ»ΠΈΡΠ΅ΡΠ°ΡΠΈΠ²Π½ΠΎΠΉ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ Π² Π΄ΠΎΠΏΠΎΠ»Π½Π΅Π½ΠΈΠ΅ ΠΊ ΠΊΠ»ΠΈΠ½ΠΈΠΊΠΎ-ΠΌΠΎΡΡΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΎΠΌΡ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ ΡΠΏΠΎΡΠΎΠ±ΡΡΠ²ΡΠ΅Ρ Π΄ΠΈΠ°Π³Π½ΠΎΡΡΠΈΠΊΠ΅ ΠΈ ΠΏΠΎΡΠ»Π΅Π΄ΡΡΡΠ΅ΠΌΡ ΠΏΡΠΎΠ³Π½ΠΎΠ·ΠΈΡΠΎΠ²Π°Π½ΠΈΡ ΡΠ΅ΡΠ΅Π½ΠΈΡ ΡΠ°ΠΊΠ° ΠΏΡΠ΅Π΄ΡΡΠ°ΡΠ΅Π»ΡΠ½ΠΎΠΉ ΠΆΠ΅Π»Π΅Π·Ρ
Endometrial dysfunction caused by chronic endometritis: Clinical and morphological aspects
Objective: to determine the clinical and morphological aspects of endometrial dysfunction caused by chronic endometritis (CE). Subjects and methods. The study included 239 reproductive-aged patients: 93 were examined for abnormal uterine bleeding; 37 patients of them had a history of miscarriage and secondary infertility, 17 patients had primary infertility (Group 1). The remaining 105 patients with infertility were examined for future in vitro fertilization (Group 2). A comparison group consisted of 41 patients with normal menstrual cycles and reproductive function across the cycle, who had separate diagnostic curettage before forthcoming surgery for uterine myoma. All the women in Groups 1 and 2 underwent standard clinical and morphological investigations, histopathological examination of the material obtained during hysteroscopy with separate diagnostic curettage (Group 1), and aspiration biopsy (Group 2) with the sections being stained with hematoxylin and eosin and by Mallory's method. Immunohistochemical diagnosis was used to assess endometrial recep tivity to steroid hormones and glycodelin. Antibodies against CD4, CD8, and CD20 were employed to study local immunity. Results. In Group 1, 52 (55.9%) of the 93 patients admitted to hospital for abnormal uterine bleeding were found to have CE on the basis of a morphopathological examination of the material after separate diagnostic study. In Group 2, CE was established in 59 (56.9%) of the 105 patients after postmortem examination. The patients with CE had a history of reproductive significant infections, such as Chlamydia, Trichomonas, Ureaplasma Mycoplasma, cytomegalovirus, papillomavirus infections, genital herpes, genital candidiasis, and bacterial vaginosis. The CE groups showed functional disorders of the endometrial glandular and surface epithelium. In the middle stage of proliferation, the expression of glycodelin in the glandular epithelial cells was detected to be moderate to strong in 83.3%. During the periovulatory period, no secretion of glycodelin is of fundamental importance for the regulation of reproductive function because this protein has contraceptive activity, by blocking the binding of sperm to the zona pellucida. Accordingly, endometrial glycodelin production during the proliferative phase, which has been identified by the authors in patients with CE, may be one of the pathogenic mechanisms for the development of infertility. In addition, the patients in Groups 1 and 2 compared with those in the comparison group were noted to have a decrease in CD4 cell counts with a simultaneous increase in CD8 expression and a reduction in CD20 levels, especially in Group 2. Conclusion. The patients with SE were identified to have endometrial dysfunction characterized by lower reception to steroid hormones, impairment in glycodelin secretion, retardation in the development of pinopods and in the phase of the menstrual cycle, and local immunity disorders. The above endometrial changes should be taken into account in the pregravid preparation of patients with CE
Endometrial dysfunction caused by chronic endometritis: Clinical and morphological aspects
Objective: to determine the clinical and morphological aspects of endometrial dysfunction caused by chronic endometritis (CE). Subjects and methods. The study included 239 reproductive-aged patients: 93 were examined for abnormal uterine bleeding; 37 patients of them had a history of miscarriage and secondary infertility, 17 patients had primary infertility (Group 1). The remaining 105 patients with infertility were examined for future in vitro fertilization (Group 2). A comparison group consisted of 41 patients with normal menstrual cycles and reproductive function across the cycle, who had separate diagnostic curettage before forthcoming surgery for uterine myoma. All the women in Groups 1 and 2 underwent standard clinical and morphological investigations, histopathological examination of the material obtained during hysteroscopy with separate diagnostic curettage (Group 1), and aspiration biopsy (Group 2) with the sections being stained with hematoxylin and eosin and by Mallory's method. Immunohistochemical diagnosis was used to assess endometrial recep tivity to steroid hormones and glycodelin. Antibodies against CD4, CD8, and CD20 were employed to study local immunity. Results. In Group 1, 52 (55.9%) of the 93 patients admitted to hospital for abnormal uterine bleeding were found to have CE on the basis of a morphopathological examination of the material after separate diagnostic study. In Group 2, CE was established in 59 (56.9%) of the 105 patients after postmortem examination. The patients with CE had a history of reproductive significant infections, such as Chlamydia, Trichomonas, Ureaplasma Mycoplasma, cytomegalovirus, papillomavirus infections, genital herpes, genital candidiasis, and bacterial vaginosis. The CE groups showed functional disorders of the endometrial glandular and surface epithelium. In the middle stage of proliferation, the expression of glycodelin in the glandular epithelial cells was detected to be moderate to strong in 83.3%. During the periovulatory period, no secretion of glycodelin is of fundamental importance for the regulation of reproductive function because this protein has contraceptive activity, by blocking the binding of sperm to the zona pellucida. Accordingly, endometrial glycodelin production during the proliferative phase, which has been identified by the authors in patients with CE, may be one of the pathogenic mechanisms for the development of infertility. In addition, the patients in Groups 1 and 2 compared with those in the comparison group were noted to have a decrease in CD4 cell counts with a simultaneous increase in CD8 expression and a reduction in CD20 levels, especially in Group 2. Conclusion. The patients with SE were identified to have endometrial dysfunction characterized by lower reception to steroid hormones, impairment in glycodelin secretion, retardation in the development of pinopods and in the phase of the menstrual cycle, and local immunity disorders. The above endometrial changes should be taken into account in the pregravid preparation of patients with CE
Immunohistochemical study of Ki-67, p53 and Notch1 expressions in prostate cancer of different grades [Immunogistokhimicheskoe issledovanie ekspressii Ki-67, p53 i Notch1 v adenokartsinomakh predstatel'noi zhelezy razlichnoi stepeni zlokachestvennosti]
OBJECTIVE: To determine the prognostic value of the expression of Ki-67, p53, and Notch1 in the diagnosis of prostate cancer. MATERIAL AND METHODS: Surgical and diagnostic materials obtained from patients with benign prostatic hyperplasia, prostate intraepithelial neoplasia, and prostate cancer of different grades underwent studies; a total of 98 patients were examined. Cell antigens were determined using rabbit anti-Ki-67 monoclonal antibodies (Clone SP6), mouse anti-p53 monoclonal antibodies (Clone Y5), and recombinant anti-Notch1 antibodies (Clone EP1238Y). RESULTS: Comparing Ki-67, p53, and Notch1 expression indices revealed statistically significant differences. There was a significant direct correlation between Ki-67 and p53 expression indices and prostate cancer grade groups based on the morphological Gleason grading system (rs=0.736; p<0.001; rs=0.682; p=0.035, respectively) and a strong inverse correlation between Notch1 expression and prostate cancer grade groups (rs= -0.425, p<0.001). CONCLUSION: Notch1 expression in conjunction with the low proliferative activity of cells and the absence of p53 (Y5) protein may suggest that the mechanism of apoptosis is preserved in tumor cells, which expands indications for medical treatment of prostate cancer.Π¦ΠΠΠ¬ ΠΠ‘Π‘ΠΠΠΠΠΠΠΠΠ―: ΠΠΏΡΠ΅Π΄Π΅Π»ΠΈΡΡ ΠΏΡΠΎΠ³Π½ΠΎΡΡΠΈΡΠ΅ΡΠΊΡΡ Π·Π½Π°ΡΠΈΠΌΠΎΡΡΡ ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΠΈ Ki-67, p53 ΠΈ Notch1 Π² Π΄ΠΈΠ°Π³Π½ΠΎΡΡΠΈΠΊΠ΅ ΡΠ°ΠΊΠ° ΠΏΡΠ΅Π΄ΡΡΠ°ΡΠ΅Π»ΡΠ½ΠΎΠΉ ΠΆΠ΅Π»Π΅Π·Ρ. ΠΠΠ’ΠΠ ΠΠΠ Π ΠΠΠ’ΠΠΠ«: ΠΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ ΠΎΠΏΠ΅ΡΠ°ΡΠΈΠΎΠ½Π½ΡΠΉ ΠΈ Π΄ΠΈΠ°Π³Π½ΠΎΡΡΠΈΡΠ΅ΡΠΊΠΈΠΉ ΠΌΠ°ΡΠ΅ΡΠΈΠ°Π», ΠΏΠΎΠ»ΡΡΠ΅Π½Π½ΡΠΉ ΠΎΡ ΠΏΠ°ΡΠΈΠ΅Π½ΡΠΎΠ² Ρ Π΄ΠΎΠ±ΡΠΎΠΊΠ°ΡΠ΅ΡΡΠ²Π΅Π½Π½ΠΎΠΉ Π³ΠΈΠΏΠ΅ΡΠΏΠ»Π°Π·ΠΈΠ΅ΠΉ, ΠΏΡΠΎΡΡΠ°ΡΠΈΡΠ΅ΡΠΊΠΎΠΉ ΠΈΠ½ΡΡΠ°ΡΠΏΠΈΡΠ΅Π»ΠΈΠ°Π»ΡΠ½ΠΎΠΉ Π½Π΅ΠΎΠΏΠ»Π°Π·ΠΈΠ΅ΠΉ ΠΈ ΡΠ°ΠΊΠΎΠΌ ΠΏΡΠ΅Π΄ΡΡΠ°ΡΠ΅Π»ΡΠ½ΠΎΠΉ ΠΆΠ΅Π»Π΅Π·Ρ ΡΠ°Π·Π»ΠΈΡΠ½ΠΎΠΉ ΡΡΠ΅ΠΏΠ΅Π½ΠΈ Π·Π»ΠΎΠΊΠ°ΡΠ΅ΡΡΠ²Π΅Π½Π½ΠΎΡΡΠΈ, Π²ΡΠ΅Π³ΠΎ 98 ΠΏΠ°ΡΠΈΠ΅Π½ΡΠΎΠ². ΠΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΠ΅ ΠΊΠ»Π΅ΡΠΎΡΠ½ΡΡ
Π°Π½ΡΠΈΠ³Π΅Π½ΠΎΠ² ΠΎΡΡΡΠ΅ΡΡΠ²Π»ΡΠ»ΠΎΡΡ ΠΏΡΠΈ ΠΏΠΎΠΌΠΎΡΠΈ ΠΌΠΎΠ½ΠΎΠΊΠ»ΠΎΠ½Π°Π»ΡΠ½ΡΡ
ΠΊΡΠΎΠ»ΠΈΡΡΠΈΡ
Π°Π½ΡΠΈΡΠ΅Π» ΠΊ Π±Π΅Π»ΠΊΡ Ki-67 (Clone SP6), ΠΌΡΡΠΈΠ½ΡΡ
Π°Π½ΡΠΈΡΠ΅Π» ΠΊ Π±Π΅Π»ΠΊΡ p53 (Clone Y5), ΡΠ΅ΠΊΠΎΠΌΠ±ΠΈΠ½Π°Π½ΡΠ½ΡΡ
Π°Π½ΡΠΈΡΠ΅Π» ΠΊ Π±Π΅Π»ΠΊΡ Notch1 (Clone EP1238Y). Π ΠΠΠ£ΠΠ¬Π’ΠΠ’Π«: ΠΡΡΠ²Π»Π΅Π½Ρ ΡΡΠ°ΡΠΈΡΡΠΈΡΠ΅ΡΠΊΠΈ Π·Π½Π°ΡΠΈΠΌΡΠ΅ ΡΠ°Π·Π»ΠΈΡΠΈΡ ΠΏΡΠΈ ΡΡΠ°Π²Π½Π΅Π½ΠΈΠΈ ΠΏΠΎΠΊΠ°Π·Π°ΡΠ΅Π»Π΅ΠΉ ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΠΈ Ki-67, p53 ΠΈ Notch1. Π£ΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½Π° Π΄ΠΎΡΡΠΎΠ²Π΅ΡΠ½Π°Ρ ΠΏΡΡΠΌΠ°Ρ ΠΊΠΎΡΡΠ΅Π»ΡΡΠΈΠΎΠ½Π½Π°Ρ ΡΠ²ΡΠ·Ρ ΠΌΠ΅ΠΆΠ΄Ρ ΠΏΠΎΠΊΠ°Π·Π°ΡΠ΅Π»ΡΠΌΠΈ ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΠΈ Ki-67, p53 ΠΈ ΠΏΡΠΎΠ³Π½ΠΎΡΡΠΈΡΠ΅ΡΠΊΠΈΠΌΠΈ Π³ΡΡΠΏΠΏΠ°ΠΌΠΈ ΡΠ°ΠΊΠ° ΠΏΡΠ΅Π΄ΡΡΠ°ΡΠ΅Π»ΡΠ½ΠΎΠΉ ΠΆΠ΅Π»Π΅Π·Ρ, ΠΎΡΠ½ΠΎΠ²Π°Π½Π½ΡΠΌΠΈ Π½Π° ΠΌΠΎΡΡΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΎΠΉ ΠΊΠ»Π°ΡΡΠΈΡΠΈΠΊΠ°ΡΠΈΠΈ ΠΏΠΎ ΠΠ»ΠΈΡΠΎΠ½Ρ (rs=0,736, p<0,001; rs=0,682, p=0,035 ΡΠΎΠΎΡΠ²Π΅ΡΡΡΠ²Π΅Π½Π½ΠΎ), Π° ΡΠ°ΠΊΠΆΠ΅ Π²ΡΡΠ²Π»Π΅Π½Π° ΡΠΈΠ»ΡΠ½Π°Ρ ΠΎΠ±ΡΠ°ΡΠ½Π°Ρ ΡΠ²ΡΠ·Ρ ΠΌΠ΅ΠΆΠ΄Ρ ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΠ΅ΠΉ Notch1 ΠΈ ΠΏΡΠΎΠ³Π½ΠΎΡΡΠΈΡΠ΅ΡΠΊΠΈΠΌΠΈ Π³ΡΡΠΏΠΏΠ°ΠΌΠΈ ΡΠ°ΠΊΠ° ΠΏΡΠ΅Π΄ΡΡΠ°ΡΠ΅Π»ΡΠ½ΠΎΠΉ ΠΆΠ΅Π»Π΅Π·Ρ (rs=β0,425, p<0,001). ΠΠ°ΠΊΠ»ΡΡΠ΅Π½ΠΈΠ΅. ΠΠΊΡΠΏΡΠ΅ΡΡΠΈΡ Notch1 Π² ΡΠΎΡΠ΅ΡΠ°Π½ΠΈΠΈ Ρ Π½ΠΈΠ·ΠΊΠΎΠΉ ΠΏΡΠΎΠ»ΠΈΡΠ΅ΡΠ°ΡΠΈΠ²Π½ΠΎΠΉ Π°ΠΊΡΠΈΠ²Π½ΠΎΡΡΡΡ ΠΊΠ»Π΅ΡΠΎΠΊ ΠΈ ΠΎΡΡΡΡΡΡΠ²ΠΈΠ΅ΠΌ Π±Π΅Π»ΠΊΠ° p53 (Y5) ΠΌΠΎΠΆΠ΅Ρ ΡΠ²ΠΈΠ΄Π΅ΡΠ΅Π»ΡΡΡΠ²ΠΎΠ²Π°ΡΡ ΠΎ ΡΠΎΡ
ΡΠ°Π½Π½ΠΎΡΡΠΈ ΠΌΠ΅Ρ
Π°Π½ΠΈΠ·ΠΌΠ° Π°ΠΏΠΎΠΏΡΠΎΠ·Π° Π² ΠΎΠΏΡΡ
ΠΎΠ»Π΅Π²ΡΡ
ΠΊΠ»Π΅ΡΠΊΠ°Ρ
, ΡΡΠΎ ΡΠ°ΡΡΠΈΡΡΠ΅Ρ ΠΏΠΎΠΊΠ°Π·Π°Π½ΠΈΡ ΠΊ ΠΊΠΎΠ½ΡΠ΅ΡΠ²Π°ΡΠΈΠ²Π½ΠΎΠΌΡ Π»Π΅ΡΠ΅Π½ΠΈΡ ΡΠ°ΠΊΠ° ΠΏΡΠ΅Π΄ΡΡΠ°ΡΠ΅Π»ΡΠ½ΠΎΠΉ ΠΆΠ΅Π»Π΅Π·Ρ
SOCIO-POLITICAL AND HISTORICAL TRANSFORM CONSTITUTIONALISM IN THE MODERN WORLD
The article explores different points of view regarding the dialectics of the socio-political and historical development of global constitutionalism and its relationship with changes in the socio-political and state-legal system on a planetary scale (as a historically integral part in the development of society at any stage of the historical era, opposite to autarkic tendencies; as a phenomenon inherent in modern society). In this regard, the paper concludes that the socio-political and historical transformations of global constitutionalism as a social concept in the near future will largely determine the main directions of the socio-political and state-legal development of nation-states. The purpose of the research: from the standpoint of the socio-philosophical methods of cognition of social reality and the ideas reflecting it, to analyze the socio-political and historical transformations of global constitutionalism in the modern world. Object of research: the phenomenon of globalization of socio-political, state-legal and financial-economic development of national societies and states as a phenomenon of social reality, highlighted in the social concept of global constitutionalism
Immunohistochemical expression of nanog protein in prostate cancer cells of distinct grade groups [ΠΠΠΠ£ΠΠΠΠΠ‘Π’ΠΠ₯ΠΠΠΠ§ΠΠ‘ΠΠΠ― ΠΠΠ‘ΠΠ ΠΠ‘Π‘ΠΠ― ΠΠΠΠΠ NANOG Π ΠΠΠΠ’ΠΠΠ₯ Π ΠΠΠ ΠΠ ΠΠΠ‘Π’ΠΠ’ΠΠΠ¬ΠΠΠ ΠΠΠΠΠΠ« Π ΠΠΠΠΠ§ΠΠ«Π₯ ΠΠ ΠΠΠΠΠ‘Π’ΠΠ§ΠΠ‘ΠΠΠ₯ ΠΠ Π£ΠΠ]
Prostate cancer is the most common type of cancer among men, which is mainly due to extensive use of screening tests and high total number of prostate biopsies. Verification of tumors with poorer prognosis is the primary goal of prostate cancer management. The study was aimed to determine the clinical and morphological associations and the prognostic value of the Nanog protein expression in prostate cancer of distinct Grade Groups. We used the prostate tissue specimens obtained during surgery, and the biopsy specimens, the total of 89 cases. Histological and immunohistochemical assessment was performed using antibodies to Ki-67 and Nanog. Correlations between the expression of markers and the Grade Groups were revealed using the Spearman's rank correlation coefficient, and the correlation with clinical and morphological characteristics was determined using the chi-squared test (Ο2). There was a positive correlation between the expression of Ki-67 and Nanog, and the Grade Group numerical order (rs = 0.619, p < 0.001 and rs = 0.786, p < 0.001 respectively). We managed to find the relationship between the high Nanog expression and the extraprostatic extension (p = 0.041). High expression of Nanog protein in the prostate cancer cells was associated with a higher-grade adenocarcinoma and indicated a poor prognosis. Β© 2020 Pirogov Russian National Research Medical University. All rights reserved