Segment-scale volcanic episodicity : evidence from the North Kolbeinsey Ridge, Atlantic

The upper oceanic crust is produced by magmatism at mid-ocean ridges, a process thought to be characterized by cyclic bouts of intense magmatic activity, separated by periods when faulting accommodates most or even all of the plate motion. It is not known whether there is a distinct periodicity to such magmatic–tectonic cycles. Here we present high-resolution sidescan sonar data from the neovolcanic zone of the North Kolbeinsey Ridge, a shallow slow-spreading ridge where high glacial and steady post-glacial sedimentation rates allow relative flow ages to be determined with a resolution of around 2 kyr using backscatter amplitude as a proxy for sediment thickness and hence age. We identify 18 lava flow fields covering 40% of the area surveyed. A group of 7 flow fields showing the highest (and similar) backscatter intensity are scattered along 75 km of axial valley surveyed, suggesting that at least this length of the segment was magmatically active within a 1.2 kyr time window. Based on conservative age estimates for all datable flows and estimated eruption volumes, the post-glacial volcanic activity imaged is insufficient to maintain crustal thickness, implying that episode(s) of enhanced activity must have preceded the volcanism we image

Bound Tris confounds the identification of binding site residues in a paraquat single chain antibody

We produced an anti-paraquat single chain antibody (scFv) to investigate its potential use in immunotherapy for paraquat poisoning. However, this scFv was expressed in an insoluble form and only displayed moderate binding affinity. An earlier examination of the pH dependence of antigen binding by the parent paraquat-specific mAb (7D7-3) suggested that the electrostatic effects of a tyrosine residue were important. The aims of the current study were to obtain expression of a soluble scFv (D10) and to increase its binding affinity. The former was achieved by expression in a phagemid vector. Site-directed mutagenesis of tyrosine residues in CDR H3 did not result in improved affinity for paraquat, suggesting that the original pH dependence required re-examination. Nuclear magnetic resonance studies of 7D7-3 Fab revealed that the original observation of the pH-dependent paraquat binding with a mid-point of ~pH 8.9 was due to tightly bound Tris. It appears that as Tris is titrated to a neutral species the energetically unfavourable juxtaposition of its positive charge with that of paraquat is reduced. These findings have broad implications in the interpretation of the pH or salt dependence of any antibody-antigen interaction which should be made cautiously and with regard to the possible interference of buffer components introduced during the preparation of the antibody

Necrotroph Attacks on Plants: Wanton Destruction or Covert Extortion?

Necrotrophic pathogens cause major pre- and post-harvest diseases in numerous agronomic and horticultural crops inflicting significant economic losses. In contrast to biotrophs, obligate plant parasites that infect and feed on living cells, necrotrophs promote the destruction of host cells to feed on their contents. This difference underpins the divergent pathogenesis strategies and plant immune responses to biotrophic and necrotrophic infections. This chapter focuses on Arabidopsis immunity to necrotrophic pathogens. The strategies of infection, virulence and suppression of host defenses recruited by necrotrophs and the variation in host resistance mechanisms are highlighted. The multiplicity of intraspecific virulence factors and species diversity in necrotrophic organisms corresponds to variations in host resistance strategies. Resistance to host-specific necrotophs is monogenic whereas defense against broad host necrotrophs is complex, requiring the involvement of many genes and pathways for full resistance. Mechanisms and components of immunity such as the role of plant hormones, secondary metabolites, and pathogenesis proteins are presented. We will discuss the current state of knowledge of Arabidopsis immune responses to necrotrophic pathogens, the interactions of these responses with other defense pathways, and contemplate on the directions of future research