PARENTS’ MOTIVATIONAL INTEREST OF IN RHYTHMIC GYMNASTICS

For any parents, it is important to support child in their activities. When parents help and support their children it is much easier and more interesting for them to do things, they feel the support and interest of the parents themselves in what they do and the desire to get better, to perform tasks even more diligently to please close people. In this article, an analysis of important things in gymnastics classes for parents will be presentedКогда родители помогают и поддерживают своих детей, им намного легче и интереснее делать что-то. Они чувствуют поддержку и интерес самих родителей в том, что они делают, и желание делать еще лучше, выполнить задачи еще более старательно, чтобы понравиться близким людям. В этой статье будет представлен анализ важных вещей в классах гимнастики для родителей

Organization of Educational Environment at Pedagogical University: Concepts and Scenario of Changes

The subjects of education satisfaction analysis with the teacher training results at the University was carried out. The training deficiencies and new challenges were identified, which made it possible to determine the guidelines for the development of pedagogical university. A variant to transform the organizational scheme of communications between university departments was presented to increase the efficiency of educational environment organization for professional training of future teachers

ОБРАЗОВАНИЕ КОМПЛЕКСОВ МЕДИ(II) С ВОДОРАСТВОРИМЫМ β-ФУРФУРОЛОКСИМОМ

The paper is devoted to the interaction of copper(II) chloride with water soluble β-furfuraldoxime (N-(furan-2-ylmethyliden)hydroxylamine) in hydrochloric solutions. It was been revealed that the degree of copper(II) precipitation from aqueous solutions and hydrochloric solutions with the pH of 4 M HCl by this reagent is not higher than 52% even when the precipitant is used in abundance. These dependencies has been compared to the similar results of palladium(II) precipitation obtained when all other conditions were equal. It has been discovered that various complexes are formed when Cu(II) reacts with a β-furfuraldoxime and that it depends on the acidity of the solution. In weak acid solutions (0.01-0.1 M HCl) the «green» complex of composition [CuL4Cl2] was obtained and in this complex the oxime is coordinated with the central atom by an oxygen atom of the oxime group. The so-called «red» complex of composition [CuL2Cl2] is formed in the pH range of 1-4 M HCl and in ethanol. In this complex the coordination of ligand molecules is realized by the oxygen atom of the furan ring and by the oxygen of the oxime group. Reagent N-(furan-2-ylmethyliden)hydroxylamine can be used for practical quantitative release of palladium from hydrochloric solutions. However, it does not provide the separation of copper and palladium in their joint presence.В работе изучено взаимодействие хлорида меди(II) с водорастворимым β-фурфуролоксимом (N-(фуран-2-илметилиден)гидроксиламином) в солянокислых растворах. Выявлено, что степень осаждения меди(II) данным реагентом из водных растворов и до достижения кислотности 4 М HCl не превышает 52% даже при использовании избытка осадителя. Полученные зависимости сопоставлены с аналогичными данными для осаждения палладия(II) при прочих равных условиях. Установлено, что при взаимодействии Cu(II) с β-фурфуролоксимом в слабокислых растворах (0.01-0.1 М HCl) получается «зеленый» комплекс состава [CuL4Cl2], в котором оксим координируется к центральному атому через атом кислорода оксимной группы. В диапазоне кислотности 1-4 М HCl, а также в среде этилового спирта происходит образование так называемого «красного» комплекса состава [CuL2Cl2], в котором координация молекул лиганда осуществляется через атом кислорода фуранового кольца и кислорода оксимной группы. Реагент N-(фуран-2-илметилиден)-гидроксиламин может быть использован для практически количественного выделения палладия из солянокислых растворов, однако он не обеспечивает разделения меди и палладия при их совместном присутствии

Favorable Outcome of Severe Acute Methadone Poisoning (Clinical Case)

Purpose: to contribute to relief of metabolic disorders associated with hypoxia by including a succinate correcting tissue metabolism into the scheme of intensive care of acute severe methadone poisoning complicated by the crush syndrome.Materials and methods. We examined the dynamics of clinical manifestation of acute methadone poisoning complicated by crush syndrome: changes in clinical and biochemical parameters, coagulation test findings, blood gas composition and acid-base state, ECG and X-ray findings.Results. By the 7th day after patient's admission in the ICU, stabilization of hemodynamics, recovery of spontaneous breathing and consciousness, reduction of swelling and the beginning of the formation of pressure ulcers were observed; on the 8 the day, restoration of urination and characteristics of urine were registered. On the 12th day, the patient was transferred to the toxicology unit being in a state of moderate severity and demonstrating positive changes in all clinical parameters.Conclusion. The inclusion of a drug with antioxidant/antihypoxant properties in the infusion therapy scheme allows to relieve metabolic disorders associated with hypoxia, and thereby increase the effectiveness of treatment: i.e. to relief clinical and laboratory manifestations and reduce the ICU stay

Predictors of thromboembolic complications in patients with severe SARS-CoV-2 coronavirus infection

Aim. To identify predictors of the development of thromboembolic complications (TECs) in patients with severe SARS-CoV-2 coronavirus infection. Materials and methods. A single-center observational retrospective study included 1634 patients with a confirmed diagnosis of SARS-CoV-2 coronavirus infection. The patients were divided into 2 groups depending on the availability of the feasibility study. The criterion for inclusion of patients in the main group was the presence of venous feasibility studies in 127 patients (group I), the comparison group consisted of 1507 patients in whom the course of COVID-19 was not complicated by the development of feasibility studies (group II). Results. When performing computed tomography of the chest organs, it was revealed that patients with a feasibility study had a higher percentage of lung tissue damage than patients in the comparison group: 55% [37.5; 67.5] and 37.5% [25.0; 47.5], respectively (p0.001). The average values of C-reactive protein in I patients group were 129 [60.1; 211] ng/l, which was significantly higher than in II patients group – 41.0 [12.2; 97.6] ng/l (p0.001), interleukin-6 – 176 [52.9; 471] pg/ml and 39.4 [11.0; 107] pg/ml (p0.001), respectively. A one-factor regression analysis proved a significant contribution of comorbid pathology to the development of feasibility studies in patients with COVID-19. The presence of three nosologies at the same time: arterial hypertension, coronary heart disease (CHD) and chronic kidney disease increased the probability of a feasibility study by 4.81 times (odds ratio 4.8117, 95% confidence interval 3.2064–7.2207), in patients with arterial hypertension, CHD, chronic kidney disease and type 2 diabetes – by 5.63 times (odds ratio 5.6321, 95% confidence interval 3.1870–9.9531). Conclusion. The presence of severe comorbid pathology significantly increased the risk of developing a feasibility study in patients with COVID-19. The most significant predictors of the development of feasibility studies in patients with severe SARS-CoV-2 coronavirus infection. They are: CHD, arterial hypertension and type 2 diabetes

Особенности применения предметно-языкового интегрированного обучения в профессиональном образовании (на примере факультета сервиса и туризма)

The article raises the question of the need of teaching a number of profile disciplines in a foreign language (the subject-language integrated training) for students of the faculty of service and tourism. After graduating from a higher education institution, graduates of the Bachelor's and Master's degree programmes of specialisms «Service», «Tourism», and «Hospitality» have to know a foreign language at the level sufficient for the solution of problems of interpersonal and cross-cultural interaction, and for the implementation of business communications in professional activity: working in a contact zone with the consumer, consulting, coordinating, maintaining documentation, etc. The article describes the experience of teaching the course «Restaurant and Hotel Service» – one of the profile disciplines taught at the faculty of service and tourism of the FGBOU VO «Volga region state academy of physical culture, sport and tourism» – in English. The method of delivering lectures involving dialogue, not only in the direction Teacher ↔ Student but also Student ↔ Student, when students become active participants in delivering lecture material, is presented in the article. While conducting practical training, two methods were used. At the core of the first method there is preliminary preparation of presentations on given subjects by a subgroup of students (in 4–5 people), with further performance and discussion of material in English and Russian. The second method of conducting practicals is based on problem training with the solution of cases directly at practical sessions without preliminary training of students. The comparative analysis of the results of the entering (at the beginning of a semester) and intermediate (at the end of a semester) students' tests regarding their language competence on the basis of the All-European scale (Common European Framework of Reference), and the questionnaire of the students showed the efficiency of teaching profile disciplines in English. The most pronounced dynamics of language competence was shown by the students of the experimental group. The prospects of the subject- language integrated training, including the use of the «Flipped Class» method, are presented in the article.В статье поднимается вопрос о необходимости преподавания ряда профильных дисциплин на иностранном языке (предметно-языковое интегрированное обучение) для студентов факультета сервиса и туризма. Выпускники бакалавриата и магистратуры направлений «Сервис», «Туризм», «Гостиничное дело» по окончании вуза должны владеть иностранным языком на уровне, достаточном как для решения задач межличностного и межкультурного взаимодействия, так и для осуществления деловых коммуникаций в профессиональной деятельности: работа в контактной зоне с потребителем, консультирование, согласование, ведение документации и т. д. В статье приводится опыт ведения курса «Ресторанно-гостиничный сервис» – одной из профильных дисциплин, преподаваемых на факультете сервиса и туризма ФГБОУ ВО «Поволжская государственная академия физической культуры, спорта и туризма» на английском языке. Представлен метод проведения лекционных занятий с применением ведения диалога не только в направлении Преподаватель ↔ Студент, но и Студент ↔ Студент, когда обучающиеся становятся активными участниками изложения лекционного материала. При ведении практических занятий использовалось два метода. В основе одного метода лежит предварительная подготовка презентаций по представленным темам подгруппой студентов (по 4–5 человек) с дальнейшим выступлением и обсуждением материала на английском и русском языках. Второй метод ведения практических занятий основан на проблемном обучении с решением кейсов непосредственно на практическом занятии без предварительной подготовки студентов. Cравнительный анализ результатов входящего (начало семестра) и промежуточного (конец семестра) тестирований студентов на предмет владения ими языковой компетенции на основе общеевропейской шкалы (Common European Framework of Reference) и анкетирования студентов показали эффективность преподавания профильных дисциплин на английском языке. Наиболее ярко выраженную динамику языковой компетенции показали студенты экспериментальной группы. В статье представлены перспективы предметно-языкового интегрированного обучения, в том числе с использованием метода «перевернутого класса»

Hepatitis B virus and site-specific nucleases: effects of genetic modifications in CRISPR/Cas9 on antiviral activity

Chronic hepatitis B is a severe liver disease caused by persistent infection of hepatitis B virus in human hepatocytes. Chronic hepatitis B is one of the most common diseases in the world. According to recent estimations, more than 250 million people are chronically infected and more than 1 million of people die annually due to consequences of chronic hepatitis B: liver cirrhosis and hepatocellular carcinoma. The key factor of hepatitis B virus persistency is a special form of viral genome called circular covalently closed DNA. Current therapeutics suppress viral replication but have no effect on circular covalently closed DNA as it exists in the nuclei of hepatocytes as a minichromosome and is not accessible for therapeutics. Commonly, viral reactivation occurs after cessation of treatment. Therefore, duration of antiviral treatment is supposed to be indefinitely long. One of the most promising approaches to target circular covalently closed DNA is the technology of site-specific nucleases CRISPR/Cas9 from Streptococcus pyogenes. A short guide RNA recruits an SpCas9 protein to the viral genome and induces generation of DNA double strand breaks. However, there are several limitations of CRISPR/Cas9 hampering translation of this technology into the clinic. First, efficacy of CRISPR/Cas9 needs to be improved. Second, CRISPR/Cas9-mediated off-target mutagenesis represents a menacing problem which has to be addressed. To overcome these limitations, several approaches have been devised to improve CRISPR/Cas9 activity (modification of guide RNAs) and reduce off-target mutagenesis (a Cas9 protein with enhanced specificity, eSpCas9). In this study, we compared antiviral activity of a classic SpCas9 with an eSpCas9 system as well as analyzed effects of gRNAs modification on anti-HBV effects. Here, we demonstrated that SpCas9 has the highest antiviral potency, reducing transcription and replication of HBV over 90%. Hepatitis B virus covalently closed circular DNA declined over 90% post CRISPR/Cas9 transfection. Although it was previously shown that modified guide RNAs increase nucleolytic activity of CRISPR/Cas9, our results indicated that this modification impairs antiviral activity of CRISPR/Cas9. To conclude, CRISPR/Cas9 effectively suppress viral replication and transcription per se. Described modifications do not potentiate antiviral activity of CRISPR/Cas9 system and should not be used for development of future therapeutics. The best strategy to improve CRISPR/Cas9 efficacy is to design new highly effective guide RNAs

Evaluation of the occupational risk of dock engineer of the seaport in Saint-Petersburg city

The aim of the study is assessment of harmful factors affecting the dock engineer’s health basing on the models of evaluation of the occupational risk to develop preventive measures in following.Целью исследования является оценка влияния вредных факторов на здоровье докеров — механизаторов на основе использования моделей профессионального риска для последующей разработки профилактических мероприятий

СОРБЦИЯ ПАЛЛАДИЯ (II) ИЗ АЗОТНОКИСЛЫХ РАСТВОРОВ КРЕМНЕЗЕМОМ, МОДИФИЦИРОВАННЫМ АМИНОГРУППАМИ

Sorption of Pd(II), Ag(I) and Cu(II) from nitric acid solutions by silica chemically modified with γ-aminopropyltriethoxysilane was studied. Based on the research of solid phase by IR & X-ray photoelectron spectroscopy, as well as thermogravimetry, it was suggested that palladium sorption from nitric acid solutions includes the following processes: coordination of palladium ions to nitrogen atoms of the functional group, reaction of palladium ions with the silica matrix and formation of polynuclear complexes. It was also proposed to use 5 % thiourea solution in 0,1 M HCl as a palladium desorbent. It was determined that the time of constant sorption rate settling in static conditions was (min): for Ag(I) – 10, for Cu(II) – 20, Pd(II) – 30. The row of ion sorption (imbibition) from 2 M HNO3 is as follows: АAg(I) > АPd(II) >> АCu(II). At the same time, silver ions are not adsorbed from solutions with a concentration of HNO3 < 1 M thus creating prerequisites for separation of ions. Изучена сорбция Pd(II), Ag(I) и Cu(II) из азотнокислых растворов кремнеземами, химически модифицированными γ-аминопропилтриэтоксисиланом. На основании исследования твердых фаз методами ИКи рентгеновской фотоэлектронной спектроскопии, термогравиметрии высказано предположение, что при сорбции палладия из азотнокислых растворов имеют место следующие процессы: координация ионов палладия к атомам азота функциональной группы, взаимодействие ионов палладия с кремнеземной матрицей, а также образование полиядерных комплексов. В качестве десорбента палладия предложено использовать 5 %-ный раствор тиомочевины в 0,1 М HCl. Определено, что время установления постоянных значений сорбции в статических условиях составляет, мин: для Ag(I) – 10, для Cu(II) – 20, Pd(II) – 30. Ряд сорбируемости ионов в одинаковых условиях из 2 М HNO3 следующий: АAg(I) > АPd(II) >> АCu(II). При этом из растворов с концентрацией HNO3 < 1 М ионы серебра не сорбируются, что создает предпосылки для разделения ионов

The structure of the oropharyngeal genus Candida fungi community in HIVinfected patients

At the present time virtually no data are available about the structure of the genus Candida fungus able to target HIV-infected patients and serve as an etiological factor of candidiasis. The aforementioned shaped the aim of the study: to examine structure of the Candida genus community colonizing the oropharynx in HIV-infected patients with clinical manifestations of oropharyngeal candidiasis. There was conducted a microbiological study of the oropharynx in 31 HIV-infected patients (51.6% males and 48.4% females) with clinical manifestations of oropharyngeal candidiasis treated at Moscow Infectious Clinic No. 2 inpatient department in the years 2015–2017. We confirmed the diversity of the oropharyngeal Candida spp. community found in HIV-infected patients. Total 52 isolates of the genus Candida were isolated. C. albicans dominated in 57.7% cases, whereas C. glabrata prevailed (21.1%) among non-albicans species. Minor components were represented by C. tropicalis (11.5%) and C. krusei (9.6%). C. albicans and C. glabrata were sensitive to polyenes, whereas minor community components — to itroconazole and clotrimazole. The vast majority of fungal strains were resistant to fluconazole. The genus Candida community reveals a unique architecture so that any member may exist in the oropharyngeal biotope of HIV-infected patients as a monoculture or in association: homogeneous, consisting of a single species strains, or heterogeneous, formed by several species. Candida fungi in 18 patients (58.1%) were isolated as a monoculture, whereas in 13 (41.9%) subjects — in association consisting of 34 isolates (65.4% of total number), of which 16 (30.8%) and 18 (34.6%) were isolated from homogeneous and heterogeneous associations, respectively. There were identified 9 two-component associations (69.2%), and 4 (30.8%) consisting of three or more components. It turned out that pattern of the examined community was mainly determined by species composition that agrees with previous data. Most common associations were presented by C. krusei (100%) and C. albicans (73.3%). Upon that, most often C. albicans (72.7%) formed a homogeneous type of associations. Sensitivity of Candida fungi to antimycotic drugs also depended on the architecture of related community. C. albicans isolates in heterogeneous associations revealed a wide range of resistance acquired by contact with non-albicans species