Seeds of phase transition to thermoacoustic instability

Tackling the problem of emissions is at the forefront of scientific research today. While industrial engines designed to operate in stable regimes produce emissions, attempts to operate them at 'greener' conditions often fail due to a dangerous phenomenon known as thermoacoustic instability. Hazardous high amplitude periodic oscillations during thermoacoustic instability lead to the failure of these engines in power plants, aircraft, and rockets. To prevent this catastrophe in the first place, identifying the onset of thermoacoustic instability is required. However, detecting the onset is a major obstacle preventing further progress due to spatiotemporal variability in the reacting field. Here, we show how to overcome this obstacle by discovering a critical condition in certain zones of the combustor, which indicates the onset of thermoacoustic instability. In particular, we reveal the critical value of the local heat release rate that allows us to distinguish stable operating regimes from hazardous operations. We refer to these zones as seeds of the phase transition because they show the earliest manifestation of the impending instability. The increase in correlations in the heat release rate between these zones indicates the transition from a chaotic state to a periodic state. Remarkably, we found that observations at the seeds of the phase transition enable us to predict when the onset occurs, well before the emergence of dangerous large-amplitude periodic acoustic pressure oscillations. Our results contribute to the operation of combustors in more environment-friendly conditions. The presented approach is applicable to other systems exhibiting such phase transitions.Indian Institute of Technology Madrashttps://doi.org/10.13039/501100003845Federal Ministry for the Environment, Nature Conservation and Nuclear Safety and the International Climate Initiative GermanyDepartment of Science and Technology IndiaRussian Foundation for Basic Researchhttps://doi.org/10.13039/501100002261Peer Reviewe

The Flow Cytometry Study of Cellular Immunity in Rhesus Monkeys after Experimental Infection with SARS CoV 2 Virus

Cellular immunity plays an important role in the pathogenesis and formation of protective immune defense against the SARS‑CoV‑2 virus.The aim of the work was to study the cellular immunity of rhesus monkeys applying flow cytometry after experimental infection with the SARS‑CoV‑2 virus.Materials and methods. Male rhesus monkeys were intranasally inoculated with the SARS‑CoV‑2 virus, Isolate B strain and hCoV-19/Russia/SP48-1226/2020 strain (abbreviated name U-2), at a dose of 5.0 lg PFU. Using flow cytometry, the levels of 21 populations/subpopulations of mononuclear cells in the peripheral blood of animals were determined before experimental infection with the pathogen and on day 14 after infection. SARS‑CoV‑2 coronavirus RNA was assessed using real-time polymerase chain reaction. Determination of the titer of virus-neutralizing antibodies to the SARS‑CoV‑2 virus in the blood sera of animals was conducted through neutralization test evaluating the ability to suppress negative colonies.Results and discussion. Infection with Isolate B strain culture has led to an increase in the relative content of total T-lymphocytes (p˂0.2), cytotoxic T-lymphocytes (p˂0.1), as well as monocytes expressing the early activation marker CD25 (p˂0.2). The decrease in levels has been observed for total B-lymphocytes (p˂0.2) and T-helper cells (p˂0.1). Infection with the U-2 strain culture revealed an increase in the relative content of monocytes expressing the early activation marker CD25 (p˂0.2). Thus, for the first time in the Russian Federation, flow cytometry was used to study the cellular immunity of rhesus monkeys before and after experimental infection with the SARS‑CoV‑2 virus. The obtained information can be used for studying the pathogenesis of SARS‑CoV‑2 infection, course, and outcome of the disease, and developing strategies for vaccination and treatment