Induction of division and differentiation of somatic embryos in the leaf epidermis of Gaillardia picta

Somatic embroys and subsequent plant regeneration were obtained from isolated leaf epidermis of Gaillardia picta. Abaxial and adaxial epidermal peels (monolayer) from 45 days old aseptic seedlings were isolated and segments measuring 5 mm × 3 mm were cultured on B5 basal medium supplemented with various growth regulators such as naphthaleneacetic acid or indolebutyric acid and benzylaminopurine or kinetin. Within 12 h of culture the epidermal cells showed receding of cytoplasm from the walls. After 48 h of incubation 3 or 4 localized zones, each consisting of 3-8 cells that accumulated cytoplasm and stained densely, were observed. Mitotic divisions occurred in these zones on day 3 of culture and localized masses of callus were observed in 95% of the cultures after 10 days. In another 5 days, the callus differentiated somatic embryos or roots, depending on the growth regulators and their concentration in the medium

Reversal of abscisic acid induced stomatal closure by benzyl adenine

Benzyl adenine enhanced stomatal opening in isolated epidermal strips of Commelina benghalensis and Tridax procumhens. The stimulation was maximum at a concentration of 5×10−5 M BA. But kinetin had no remarkable effect on stomatal opening. The activity of benzyl adenine was observed over a narrow range of concentrations, from 10−5 M to 10−4 M. The increase in stomatal aperture was more pronounced in Commelina than in Tridax. Presence of benzyl adenine in the medium prevented the stomatal closure expected from abscisic acid and could reverse considerably the stomatal closure induced earlier by abscisic acid. It is felt that the balance between abscisic acid and cytokinins can possibly control the stomatal aperture effectively

Zero Temperature Insulator-Metal Transition in Doped Manganites

We study the transition at T=0 from a ferromagnetic insulating to a ferromagnetic metallic phase in manganites as a function of hole doping using an effective low-energy model Hamiltonian proposed by us recently. The model incorporates the quantum nature of the dynamic Jahn-Teller(JT) phonons strongly coupled to orbitally degenerate electrons as well as strong Coulomb correlation effects and leads naturally to the coexistence of localized (JT polaronic) and band-like electronic states. We study the insulator-metal transition as a function of doping as well as of the correlation strength U and JT gain in energy E_{JT}, and find, for realistic values of parameters, a ground state phase diagram in agreement with experiments. We also discuss how several other features of manganites as well as differences in behaviour among manganites can be understood in terms of our model.Comment: To be published in Europhysics Letter

Succinct Indexable Dictionaries with Applications to Encoding kk-ary Trees, Prefix Sums and Multisets

We consider the {\it indexable dictionary} problem, which consists of storing a set $S \subseteq \{0,...,m-1\}$ for some integer $m$, while supporting the operations of \Rank(x), which returns the number of elements in $S$ that are less than $x$ if $x \in S$, and -1 otherwise; and \Select(i) which returns the $i$-th smallest element in $S$. We give a data structure that supports both operations in O(1) time on the RAM model and requires ${\cal B}(n,m) + o(n) + O(\lg \lg m)$ bits to store a set of size $n$, where {\cal B}(n,m) = \ceil{\lg {m \choose n}} is the minimum number of bits required to store any $n$-element subset from a universe of size $m$. Previous dictionaries taking this space only supported (yes/no) membership queries in O(1) time. In the cell probe model we can remove the $O(\lg \lg m)$ additive term in the space bound, answering a question raised by Fich and Miltersen, and Pagh. We present extensions and applications of our indexable dictionary data structure, including: An information-theoretically optimal representation of a $k$-ary cardinal tree that supports standard operations in constant time, A representation of a multiset of size $n$ from $\{0,...,m-1\}$ in ${\cal B}(n,m+n) + o(n)$ bits that supports (appropriate generalizations of) \Rank and \Select operations in constant time, and A representation of a sequence of $n$ non-negative integers summing up to $m$ in ${\cal B}(n,m+n) + o(n)$ bits that supports prefix sum queries in constant time.Comment: Final version of SODA 2002 paper; supersedes Leicester Tech report 2002/1

Interplay of p-d and d-d charge transfer transitions in rare-earth perovskite manganites

We have performed both theoretical and experimental study of optical response of parent perovskite manganites RMnO_3 with a main goal to elucidate nature of clearly visible optical features. Starting with a simple cluster model approach we addressed the both one-center (p-d) and two-center (d-d) charge transfer (CT) transitions, their polarization properties, the role played by structural parameters, orbital mixing, and spin degree of freedom. Optical complex dielectric function of single crystalline samples of RMnO_3 (R=La, Pr, Nd, Sm, Eu) was measured by ellipsometric technique at room temperature in the spectral range from 1.0 to 5.0 eV for two light polarizations: E \parallel c and E \perp c. The comparative analysis of the spectral behavior of \varepsilon _1 and \varepsilon _2 is believed to provide a more reliable assignment of spectral features. We have found an overall agreement between experimental spectra and theoretical predictions based on the theory of one-center p-d CT transitions and inter-site d-d CT transitions. Our experimental data and theoretical analysis evidence a dual nature of the dielectric gap in nominally stoichiometric matrix of perovskite manganites RMnO_3, it is formed by a superposition of forbidden or weak dipole allowed p-d CT transitions and inter-site d-d CT transitions. In fact, the parent perovskite manganites RMnO_3 should rather be sorted neither into the CT insulator nor the Mott-Hubbard insulator in the Zaanen, Sawatzky, Allen scheme.Comment: 20 pages, 6 figure

On the origin of the various types of radio emission in GRS 1915+105

We investigate the association between the radio ``plateau'' states and the large superluminal flares in GRS 1915+105 and propose a qualitative scenario to explain this association. We identify several candidate superluminal flare events from available monitoring data on this source and analyze the contemporaneous RXTE pointed observations. We detect a strong correlation between the average X-ray flux during the ``plateau'' state and the total energy emitted in radio during the subsequent radio flare. We find that the sequence of events is similar for all large radio flares with a fast rise and exponential decay morphology. Based on these results, we propose a qualitative scenario in which the separating ejecta during the superluminal flares are observed due to the interaction of the matter blob ejected during the X-ray soft dips, with the steady jet already established during the ``plateau'' state. This picture can explain all types of radio emission observed from this source in terms of its X-ray emission characteristics.Comment: Corrected typo in the author names, contents unchanged, accepted in Ap

Two-step antiferromagnetic transition and moderate triangular frustration in Li2Co(WO4)2

We present a detailed investigation of the magnetic properties of the spin-$\frac{3}{2}$ system Li$_2$Co(WO$_4$)$_2$ by means of magnetic susceptibility and specific heat. Our experimental results show that in Li$_2$Co(WO$_4$)$_2$ a short-range antiferromagnetic (AFM) correlations appear near $\chi$$_{max}$ $\sim$ 11 K and two successive long-range AFM phase transitions are observed at T$_{N1}$$\sim$ 9 K and T$_{N2}$$\sim$ 7 K. The frustration factor, $\mid$$\Theta$$\mid$/T$_{N1}$$\sim$3, indicates that the system is moderately frustrated, which is identifiable by the broken triangular symmetry within both $ab$- and $bc$-planes for the triclinic crystal structure. The magnetic isotherm at temperatures below T$_{N2}$ shows a field-induced spin-flop transition, and a complete H-T phase diagram for the two-step AFM system is mapped. $Ab$~$initio$ band structure calculations suggest that the strongest exchange coupling does not correspond to the shortest Co-Co distance along the $a$-axis, but rather along the diagonal direction through a Co-O-W-O-Co super-superexchange path within the $bc$-planeComment: 7 Pages, 10 Figure